T6763

乙酰化胰蛋白酶 来源于牛胰腺

Type V-S, ≥8,500 BAEE units/mg protein (biuret)

• Synonym(s): Acetyltrypsin
• Enzyme Commission number: 3.4.21.4 (BRENDA, IUBMB)
• MDL编号: MFCD00082094
• NACRES: NA.54

Properties

• 生物来源: bovine pancreas
• 质量水平: 200
• 类型: Type V-S
• 形式: powder
• specific activity: ≥8,500 BAEE units/mg protein (biuret)
• UniProt登记号: Q29463
• 储存温度: −20°C
• Gene Information: cow ... TRYP8(282603)

Description

一般描述

The trypsin molecule has two domains: one is related to the enzyme active site and the tryptophan residues; the other is related to the 8-anilinonaphthalene-1-sulfonate binding.

应用

Trypsin has been used in a study to assess the ontogeny of the endocrine pancreas in the fetal/newborn baboon. Trypsin digestion and hydrogen/deuterium exchange support the transition between inward- and outward-facing conformations during the catalytic cycle of the bacterial multidrug ATP-binding cassette transporter.

生化/生理作用

Lys-acetylated trypsin is less susceptible to autolysis than the native form.

单位定义

One BAEE unit will produce a ΔA₂₅₃ of 0.001 per min at pH 7.6 at 25° C using BAEE as substrate. Reaction volume = 3.2 mL (1 cm light path).

制备说明

Prepared by the method of Labouesse, J. and Gervais, M., Eur. J. Biochem., 2, 215 (1967).

其他说明

View more information on trypsin at www.sigma-aldrich.com/enzymeexplorer.

Safety Information

• 象形图: GHS07,GHS08
• 警示用语：: Danger
• 危险声明: H315 - H319 - H334 - H335
• 预防措施声明: P302 + P352 - P305 + P351 + P338
• 危险分类: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 靶器官: Respiratory system
• 储存分类代码: 11 - Combustible Solids
• WGK: WGK 3
• 个人防护装备: dust mask type N95 (US), Eyeshields, Faceshields, Gloves