Merck
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T7409

Sigma-Aldrich

胰蛋白酶 来源于猪胰腺

lyophilized powder, Type II-S, 1,000-2,000 units/mg dry solid

CAS Number:
Enzyme Commission number:
EC 号:
MDL编号:
NACRES:
NA.54

类型

Type II-S

形式

lyophilized powder

specific activity

1,000-2,000 units/mg dry solid

分子量

23.8 kDa

储存温度

−20°C

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This Item
T1005T0303T5266
form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

specific activity

1,000-2,000 units/mg dry solid

specific activity

≥6,000 BAEE units/mg protein

specific activity

13,000-20,000 BAEE units/mg protein

specific activity

1,000-2,000 BAEE units/mg solid

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.8 kDa

mol wt

23.8 kDa

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

type

Type II-S

type

Type XI

type

Type IX-S

type

-

应用

对于胰蛋白酶消化肽,胰蛋白酶:肽的使用比例约为1:100至1:20。本品的典型用途是使贴壁细胞从培养表面脱落。 使细胞解离所需的胰蛋白酶浓度主要取决于细胞类型和培养物代数。 Trypsins也被用于细胞培养过程中的细胞再悬浮,在蛋白质组学研究中用于蛋白质的消化,以及在各种凝胶消化中。 其他的应用包括通过基于膜的技术评估结晶,以及在一项研究中确定蛋白质折叠速率和产量可以被动力学陷阱的存在所限制。

生化/生理作用

胰蛋白酶可切割赖氨酸和精氨酸残基的C末端肽段。 如果酸性残基位于切割位点的任意一侧,则该反应的水解速度减慢,并且如果脯氨酸残基位于切割位点的羧基侧,则水解停止。 胰蛋白酶活性的最佳pH条件为7-9。 胰蛋白酶还可以切割氨基酸合成衍生物的酯和酰胺键。 将EDTA添加到胰蛋白酶溶液中作为螯合剂,用于中和钙离子和镁离子以防止其抑制胰蛋白酶活性。 去除这些离子会增加酶活性。

丝氨酸蛋白酶抑制剂(包括DFP、TLCK、APMSF、AEBSEF和抑肽酶等)会抑制胰蛋白酶。

组分

胰蛋白酶由 223 个氨基酸残基组成的单链多肽组成,通过去除胰蛋白酶原的 N-末端六肽而产生,胰蛋白酶原在 Lys-lle 肽键处裂解。氨基酸序列由 6 个二硫键交联。这是胰蛋白酶的天然形式,β-胰蛋白酶。β-胰蛋白酶可自溶,在赖氨酸残基处裂解,生成 α-胰蛋白酶。胰蛋白酶是丝氨酸蛋白酶家族的一员。

注意

1 mM HCl 溶液等分试样可在-20°C 条件下稳定储存 1 年。Ca2+的存在也会减弱胰蛋白酶的自溶,维持其在溶液中的稳定性。胰蛋白酶在 2.0 M 尿素、2.0 M 盐酸胍或 0.1% (w/v) SDS 中也保留大部分活性。

单位定义

一个BAEE单位以BAEE作为底物,在25°C、pH 7.6条件下每分钟每毫升产生0.001的ΔA253 。一个BTEE单位=320个ATEE单位。反应体积=3.2mL(1cm的光径)。

制备说明

本品是可溶于 Hank 平衡盐溶液(25 mg/mL)的冻干粉末。

象形图

Exclamation markHealth hazard

警示用语:

Danger

危险分类

Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

靶器官

Respiratory system

储存分类代码

11 - Combustible Solids

WGK

WGK 1

个人防护装备

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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