Merck
All Photos(4)

U5378

Sigma-Aldrich

尿素

powder, BioReagent, for molecular biology, suitable for cell culture

Synonym(s):
碳酰二胺, 碳酰胺
Linear Formula:
NH2CONH2
CAS Number:
Molecular Weight:
60.06
Beilstein:
635724
EC 号:
MDL编号:
PubChem化学物质编号:
NACRES:
NA.31

质量水平

200

等级

for molecular biology

产品线

BioReagent

测定

≥98%

形式

powder

technique(s)

cell culture | mammalian: suitable

mp

132-135 °C (lit.)

溶解性

H2O: soluble 480 mg/mL, clear, colorless (8M)

密度

1.335 g/mL at 25 °C (lit.)

痕量阴离子

chloride (Cl-): ≤5 ppm

痕量阳离子

Cu: ≤1 ppm
Fe: ≤5 ppm
Pb: ≤2 ppm

吸光度

≤0.06 at 260 at 6 M
≤0.06 at 280 at 6 M

异质活性

DNase, NICKase, RNase and protease, none detected

SMILES string

NC(N)=O

InChI

1S/CH4N2O/c2-1(3)4/h(H4,2,3,4)

InChI key

XSQUKJJJFZCRTK-UHFFFAOYSA-N

Gene Information

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应用

  • 尿素已可用于蛋白质重悬缓冲液和蛋白质变性。
  • 它已可用于通过含有尿素的聚丙烯酰胺凝胶分离蛋白质。
  • 它被用作研究不同浓度尿素和不同pH值下未折叠蛋白的构象。
用于蛋白质变性以及作为不溶性或变性蛋白的温和增溶剂。可用于从已由 6M 盐酸胍变性的样品(如包涵体)复性蛋白质。可与盐酸胍和二硫苏糖醇 (DTT) 一起用于复性变性蛋白以恢复其天然或活性形式。

包装

1 kg in poly bottle
100, 500 g in poly bottle
5 kg in poly drum

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(F)

Not applicable

闪点(C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)

Certificate of Analysis

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Certificate of Origin

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