Merck

U5382

Sigma-Aldrich

泛素 人

≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), essentially salt-free, lyophilized powder

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CAS号:
MDL编号:
NACRES:
NA.32

生物来源

human

质量水平

重组

expressed in E. coli (N-terminal FLAG® tagged)

检测方案

≥95% (SDS-PAGE)

形式

essentially salt-free, lyophilized powder

分子量

10 kDa

technique(s)

mass spectrometry (MS): suitable

溶解性

0.05 M Tris pH 7.5: ≥10 mg/mL

UniProt登记号

储存温度

−20°C

Gene Information

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此商品
U5507U6253T8690
Ubiquitin human ≥95% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal FLAG® tagged), essentially salt-free, lyophilized powder

Sigma-Aldrich

U5382

泛素 人

Ubiquitin human recombinant, expressed in E. coli (N-terminal histidine tagged)

Sigma-Aldrich

U5507

泛素 人

Thioredoxin human ≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg

Sigma-Aldrich

T8690

硫氧还蛋白 人

recombinant

expressed in E. coli (N-terminal FLAG® tagged)

recombinant

expressed in E. coli (N-terminal histidine tagged)

recombinant

-

recombinant

expressed in E. coli (N-terminal histidine tagged)

assay

≥95% (SDS-PAGE)

assay

≥95% (GE)

assay

≥98% (SDS-PAGE)

assay

≥90% (SDS-PAGE)

form

essentially salt-free, lyophilized powder

form

lyophilized powder

form

essentially salt-free, lyophilized powder

form

essentially salt-free, lyophilized powder

mol wt

10 kDa

mol wt

10.7 kDa

mol wt

-

mol wt

-

technique(s)

mass spectrometry (MS): suitable

technique(s)

ligand binding assay: suitable

technique(s)

western blot: suitable

technique(s)

activity assay: suitable

一般描述

泛素是一种高度保守的球蛋白,表面有赖氨酸。C末端包含Leu-Arg-Gly-Gly结构基序。

应用

N端FLAG标签泛素可取代泛素,用于形成多聚泛素-蛋白结合物。FLAG标签可用于在抗-FLAG亲和柱上分离和富集蛋白结合物,以及在蛋白免疫印迹法中通过抗-FLAG抗体检测结合物。
人泛素可用作质谱的标准品。
人类泛素已用于酵母增殖细胞核抗原(PCNA)的单泛素化 和有丝分裂阻滞1(Dma1)缺陷的体外泛素化测定

生化/生理作用

泛素是存在于真核细胞组织中的小调节蛋白。外源性泛素可刺激许多细胞系中的细胞凋亡。 人乳头瘤病毒-16的E7蛋白可通过泛素-蛋白酶体途径刺激视网膜母细胞瘤蛋白的降解。
泛素通过其C末端甘氨酸残基的ε-氨基与蛋白质的赖氨酸残基相互作用。与泛素相互作用的蛋白质通过一个三步过程进行单泛素化(mono-ubiquitination)或多单泛素化(multi-mono-ubiquitination)。泛素化调节细胞内运输和蛋白质降解,并且该途径的失衡与疾病有关。

包装

包装尺寸基于蛋白质含量

制备说明

人泛素可以以10.00-11.00mg/ml的浓度溶解在0.05M Tris-HCl中,得到澄清至轻微浑浊的无色溶液。

法律信息

FLAG is a registered trademark of Merck KGaA, Darmstadt, Germany

储存分类代码

11 - Combustible Solids

WGK

WGK 3

闪点(°F)

Not applicable

闪点(°C)

Not applicable

个人防护装备

Eyeshields, Gloves, type N95 (US)


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Acta crystallographica. Section F, Structural biology communications, 72(Pt 1), 29-35 (2016-01-12)
Ubiquitin is a small globular protein that has a considerable number of lysine residues on its surface. This results in a high surface entropy that precludes the formation of crystal-packing interactions. To date, only a few structures of the native
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Top-down proteomics, the analysis of intact proteins (instead of first digesting them to peptides), has the potential to become a powerful tool for mass spectrometric protein characterization. Requirements for extremely high mass resolution, accuracy, and ability to efficiently fragment large
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The FLAG epitope is an eight amino acid peptide (AspTyrLysAspAspAspAspLys) that is useful for immunoaffinity purification of fusion proteins. A monoclonal antibody (anti-FLAG M1) that binds the FLAG epitope in a calcium-dependent manner and requires an N-terminal FLAG sequence has

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