Merck
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V8012

Sigma-Aldrich

单克隆抗- V5 小鼠抗

clone V5-10, purified from hybridoma cell culture

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别名:
抗-V5抗体，小鼠单克隆
NACRES:
NA.56

生物来源

mouse

质量水平

200

偶联物

unconjugated

抗体形式

purified from hybridoma cell culture

antibody product type

primary antibodies

克隆

V5-10, monoclonal

浓度

~1 mg/mL

technique(s)

immunocytochemistry: 1-2 μg/mL
western blot: 0.5-1 μg/mL

运输

dry ice

储存温度

−20°C

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此商品
A7345C0992B7405
Anti-V5 antibody, Mouse monoclonal clone V5-10, purified from hybridoma cell culture

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单克隆抗- V5 小鼠抗

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抗-V5 琼脂糖亲和凝胶 小鼠抗

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Monoclonal Anti-Cy3/Cy5 小鼠抗

Anti-Digoxin−Biotin antibody, Mouse monoclonal clone DI-22, purified from hybridoma cell culture

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抗-−地高辛生物素抗体，小鼠单克隆

conjugate

unconjugated

conjugate

agarose conjugate

conjugate

unconjugated

conjugate

biotin conjugate

antibody form

purified from hybridoma cell culture

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

clone

V5-10, monoclonal

clone

V5-10, monoclonal

clone

CY-96, monoclonal

clone

DI-22, monoclonal

concentration

~1 mg/mL

concentration

-

concentration

~1.5 mg/mL

concentration

-

technique(s)

immunocytochemistry: 1-2 μg/mL, western blot: 0.5-1 μg/mL

technique(s)

affinity chromatography: suitable, immunoprecipitation (IP): suitable

technique(s)

direct ELISA: suitable, dot blot: 1-2 μg/mL using cell protein extracts labeded with Cy3 or Cy5, immunocytochemistry: suitable, immunoprecipitation (IP): suitable, microarray: suitable

technique(s)

indirect ELISA: 1:10,000 using digoxin-labeled biomolecule, western blot: 1:500 using using digoxin-labeled biomolecule

一般描述

单克隆抗-V5（小鼠IgG1同种型）分离自V5-10杂交瘤，其通过融合小鼠骨髓瘤细胞和来自与KLH结合的合成肽免疫的BALB /c小鼠的脾细胞产生。
小鼠单克隆抗V5抗体与在转染的哺乳动物细胞中表达的，或通过体外翻译产生的V5-重组融合蛋白特异性反应。
小鼠单克隆抗V5抗体与在转染的哺乳动物细胞中表达的，或通过体外翻译产生的V5-重组融合蛋白特异性反应。

特异性

识别 V5 标签（GKPIPNPLLGLDST）融合蛋白

外形

含有1％ 牛血清白蛋白和15mM 叠氮化钠的0.01M 磷酸盐缓冲盐水（pH7.4）中的溶液。

储存及稳定性

若需连续使用，可在2-8°C下保存最长一个月。若需延长储存时间，可将溶液分装并冷冻。 不建议反复冻融或存放在“无霜”冰柜中。如果长期储存时出现轻微浑浊，请在使用前通过离心澄清溶液。若工作稀释样品在12小时内未使用完，则应丢弃。

免责声明

除非我们的产品目录另有说明，否则我们的产品仅供研究使用，不得用于任何其他目的，包括但不限于未经授权的商业用途、体外诊断用途、离体或体内治疗用途或任何类型的消费或应用于人类或动物。

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

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货号
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