W0138

Sigma-Aldrich

Western Blocker溶液

for HRP detection systems

NACRES:
NA.25

质量水平

200

保质期

≥2 yr

储存温度

2-8°C

一般描述

在蛋白质通过斑点印迹或蛋白质印迹转移到 PVDF 和硝酸纤维素膜上后,蛋白质印迹封闭液可用于封闭 PVDF 和硝酸纤维素膜。进行封闭是为了减少或消除一抗或二抗的非特异性结合。

应用

蛋白质印迹封闭液已针对使用辣根过氧化物酶(HRP)偶联物的检测系统进行了优化;它也与基于碱性磷酸酶的系统兼容。
蛋白质印迹封闭液已用于封闭蛋白质印迹膜。它也已用于蛋白质印迹中抗体的稀释。

特点和优势

  • 像许多基于牛奶的封闭液一样,它不会抑制弱 HRP 信号。
  • 既可以用作封闭成分,也可以用作探测成分。
  • 提供即用型,无需制备步骤。

法律信息

Western Blocker is a trademark of Sigma-Aldrich Co. LLC

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 2

闪点(F)

Not applicable

闪点(C)

Not applicable

分析证书

原产地证书 (CofO)

Lalit P Chandravanshi et al.
International journal of developmental neuroscience : the official journal of the International Society for Developmental Neuroscience, 34, 60-75 (2014-02-13)
In view of the increasing incidences of arsenic induced health effects and the vulnerability of the developing brain to its toxic effects, studies have been carried out to investigate the mechanism of arsenic induced cholinergic alterations and understand if such...
Massimo Conese et al.
International journal of molecular sciences, 21(12) (2020-06-26)
Recently, insulin-like growth factor binding protein 6 (IGFBP-6) has been shown to play a putative role in the immune system, as monocyte-derived dendritic cells (Mo-DCs) are stimulated by hyperthermia to express IGFBP-6 at both the mRNA and protein levels. However...
A N Meleshko et al.
Experimental oncology, 35(1), 8-14 (2013-03-27)
Idiotype, the unique part of immunoglobulin molecule expressed on the surface of B-cells, represents a specific antigen for vaccination against lymphoma. We have developed a rapid method for immunoglobulin variable fragments cloning, assembling and expression of recombinant idiotype protein in...
In vitro differentiation of human bone marrow mesenchymal stem cells into hepatocyte-like cells.
Pournasr B, et al.
Archives of Iranian Medicine, 14, 244-249 (2011)
Ali Fathi et al.
Journal of proteomics, 101, 1-16 (2014-02-18)
Neural differentiation of human embryonic stem cells (hESCs) is a unique opportunity for in vitro analyses of neurogenesis in humans. Extrinsic cues through neural plate formation are well described in the hESCs although intracellular mechanisms underlying neural development are largely...

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