Merck
All Photos(3)

WGA4

Sigma-Aldrich

GenomePlex® 单细胞全基因组扩增试剂盒

Amplify genome of a single cell

Synonym(s):
Single cell whole genome amplification, Whole genome amplification
EC 号:
NACRES:
NA.55

质量水平

200

technique(s)

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

一般描述

GenomePlex® Single Cell Whole Genome Amplification Kit utilizes a proprietary technology based on random fragmentation of genomic DNA and conversion of the resulting small fragments to PCR-amplifiable library molecules flanked by universal priming sites. WGA is achieved by PCR amplification of the library molecules using universal oligonucleotide primers. This kit is designed to amplify the genome of a single cell. This rapid and straightforward method provides millionfold amplification yielding microgram quantities of genomic DNA from a single cell. Traditional single-cell whole genome amplification methods yield insufficient quantities with significantly biased representation. The kit includes all the reagents necessary for cell lysis and successive whole genome amplification. Single cells can be isolated by fluorescence-activated cell sorting (FACS), laser capture microdissection (LCM), dilution, or any other applicable method.

应用

GenomePlex® Single Cell Whole Genome Amplification Kit has been used:

  • to amplify the isolated DNA
  • in whole genome amplification (WGA)
  • to amplify the microdissected DNAs
  • for the amplification of circulating tumor cell genomic DNA from metastatic castration-resistant prostate cancer cells
  • for the detection of copy number variations (CNV) by single-cell low-coverage whole-genome sequencing (SLWGS) method
  • Gel electrophoresis
  • qPCR
  • comparative genomic hybridization (CGH) microarray
  • Short tandem repeats (STR) analysis
  • single nucleotide polymorphism (SNP) analysis

特点和优势

  • Highly yield and accuracy of DNA amplification within four hours
  • Amplification of DNA from any source such as cancer cells, epithelial cells, lymphocytes, fibroblast amniotic cells, polycarbonate fixed cells, and plant cells
  • A complete representation of the entire genome with minimal allele bias
  • WGA DNA polymerase is suitable for use with downstream applications including gel electrophoresis, qPCR, comparative genomic hybridization (CGH) microarray, short term repeat (STR) analysis, and single nucleotide polymorphism (SNP) analysis, TaqMan® assays, and microsatellite analysis

其他说明

该试剂盒随附的通用引物的序列组成具有专利权。

法律信息

本产品的使用受到如下一项或多项美国专利及其对应的境外专利声明保护:5,789,224, 5,618,711, 6,127,155以及与届满的美国专利号5,079,352对应的境外专利声明。购买本产品,即相当于依照境外的专利声明获得了一份受限制、不可转让的使用许可,即将此等数量的产品用于购买者内部的研究用途。我们未明确表示、暗示或以禁止反言的形式授予您任何其他专利声明下的权利、进行任何专利方法申请的权利、进行任何形式的商业服务的权利,包括但不限于出于收费或其他商业考虑而报告购买者的研究活动结果的权利。本产品仅适合于研究用途。如需用于Roche专利许可的诊断用途,需另外征得Roche许可。有关购买许可的更多信息,可联系Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA获取。
GenomePlex is a registered trademark of Takara Bio USA, Inc.
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Kit Components Also Available Separately

Product No.
Description
SDS

  • L104310x Single Cell Lysis & Fragmentation Buffer

  • P4850Proteinase K from Tritirachium album, buffered aqueous glycerol solution, for molecular biology, ≥800 units/mL

  • W4502Water, Nuclease-Free Water, for Molecular Biology

象形图

Health hazard

警示用语:

Danger

危险声明

预防措施声明

危险分类

Resp. Sens. 1

储存分类代码

10 - Combustible liquids

WGK

WGK 3

闪点(F)

Not applicable

闪点(C)

Not applicable

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

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