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WTA2

Sigma-Aldrich

TransPlex® Complete Whole Transcriptom Amplification Kit

DNA polymerase included, Complete Kit with optimized enzyme to amplify total RNA in <4 hours, no 3′ bias

Synonym(s):
完整全转录组扩增试剂盒
NACRES:
NA.55

质量水平

200

technique(s)

whole genome amplification: suitable

运输

wet ice

储存温度

−20°C

一般描述

经过优化的WTA2,可对福尔马林固定的石蜡包埋样品(FFPE)和其他受损或降解样本中的RNA进行扩增。全转录组扩增(WTA)技术可在4小时内对ng级的总RNA进行无3′偏差的代表性扩增。扩增产物适用于qPCR、微阵列分析和克隆等应用。WTA2试剂盒含有cDNA文库扩增所需的聚合酶。

应用

Complete Whole Transcriptome Amplification Kit is used for the following applications:
  • To establish a protocol for the simultaneous analysis of DNA and RNA viruses present in pig feces using process controlled deep sequencing.
  • Reverse transcription and cDNA amplification
  • For the synthesis and amplification of cDNA library using Genomic RNA released from immunocaptured PPV particles
  • Nucleic Acid Preparation and Deep Sequencing (The extracted nucleic acids were randomly primed for cDNA synthesis)
适于各种下游应用,包括:
  • qPCR
  • 微阵列分析
  • 克隆

特点和优势

  • 在4小时内实现高达10,000x的扩增,并且手动操作时间低于30分钟
  • 仅需20 pg的总RNA模板,即可扩增出适用于微阵列分析的cDNA
  • 含有cDNA扩增所需的所有元件
  • 实现对表达基因和外显子的线性扩增,无3′或5′偏差
  • 有效扩增单细胞或小量RNA,包括来自动物、植物或微生物的mRNA和总RNA。

原理

WTA2过程包括2个步骤。第一步,使用由拟随机3′端和通用5′端组成的非自身互补引物对样品RNA进行逆转录。在该过程中,被取代的单链将作为引物退火和延伸的新模板。所得OmniPlex cDNA文库由以通用末端序列为侧翼序列、含100-1000个碱基的随机重叠片段组成。第二步,使用WTA2聚合酶和通用末端引物对cDNA文库进行PCR扩增,生成WTA2产物。

Kit Components Also Available Separately

Product No.
Description
SDS

  • Library Synthesis Enzyme

  • Library Synthesis Solution

  • Amplification Mix

  • Library Synthesis Buffer

  • W4502Water, Nuclease-Free Water, for Molecular Biology

  • Amplification Enzyme

  • D7295Deoxynucleotide Mix, 10 mM, Molecular Biology Reagent

储存分类代码

10 - Combustible liquids

WGK

WGK 3

Certificate of Analysis

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Certificate of Origin

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