Merck

ZRB04338

Sigma-Aldrich

Anti-EGFR Antibody, clone 11E19, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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别名:
EC: 2.7.10.1, Epidermal growth factor receptor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1

生物来源

rabbit (recombinant)

质量水平

重组

expressed in HEK 293 cells

偶联物

unconjugated

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

11E19, recombinant monoclonal
monoclonal

产品线

ZooMAb® learn more

形式

lyophilized

分子量

calculated mol wt 134.28 kDa
observed mol wt ~170 kDa

species reactivity

mouse, human

包装

antibody small pack of 25 μL

环保替代产品特性

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

增强验证

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

gene knockdown: 1:100-1:250
immunocytochemistry: suitable using 1:100
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100
western blot: suitable using 1:1,000

同位素/亚型

IgG

UniProt登记号

环保替代产品分类

运输

ambient

储存温度

2-8°C

target post-translational modification

unmodified

Gene Information

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expressed in HEK 293 cells

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expressed in HEK 293 cells

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conjugate

unconjugated

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antibody form

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clone

11E19, recombinant monoclonal

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一般描述

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone 11E19 is a ZooMAb rabbit recombinant monoclonal antibody that detects human and murine Epidermal Growth Factor Receptor. It targets an epitope with in 30 amino acids from the C-terminal region.

免疫原

KLH-conjugated linear peptide corresponding to 30 amino acids from the C-terminal region of human Epidermal growth factor receptor.

应用

Anti-EGFR, clone 11E19 ZooMAb, Cat. No. ZRB04338, is a recombinant Rabbit Monoclonal Antibody that detects epidermal growth factor receptor (EGFR) and has been tested for use in Immunocytochemistry Immunohistochemistry (Paraffin), RNAi/siRNA/Gene Knockdown Analysis, and Western Blotting.
Western Blotting Analysis: A 1:1,000 dilution from a representative lot detected EGFR in A431 cell lysate.

Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected EGFR in HeLa, A431, HUVEC, and NIH 3T3 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected EGFR in human placenta tissue sections.

RNAi/siRNA/Gene Knockdown Analysis: A 1:250 dilution from a representative lot detected EGFR lysates from wild type and EGFR knockout HeLa cells.

目标描述

Epidermal growth factor receptor (UniProt: P00533; also known as EC: 2.7.10.1, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1) is encoded by the EGFR (also known as ERBB, ERBB1, HER1) gene (Gene ID: 1956) in human. EGFR is a single-pass type 1 membrane protein that is ubiquitously expressed. It is synthesized with a 24 amino acids signal peptide that is subsequently cleaved off. The mature form contains an extracellular domain (aa 25-645) a transmembrane domain (aa 646-668), and a cytoplasmic domain (aa 669-1210). Upon binding of EGF or other ligands, the receptor undergoes homo-and/or heterodimerization that stimulates its intrinsic intracellular protein-tyrosine kinase activity and leads to autophosphorylation of tyrosine residues in the C-terminal region. It then recruits adapter proteins to turn on downstream signaling cascades. EGFR is commonly overexpressed and is mutated in many human malignancies and is often associated with aggressive phenotypes. Four different isoforms of EGFR have been described that are produced by alternative splicing. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

30 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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