Merck

ZRB1330

Sigma-Aldrich

Anti-N1-Phosphohistidine (1-pHis) Antibody, clone SC1-1, ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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别名:
Phosphohistidine

生物来源

rabbit (recombinant)

质量水平

重组

expressed in HEK 293 cells

偶联物

unconjugated

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

SC1-1, monoclonal
recombinant monoclonal

产品线

ZooMAb® learn more

形式

lyophilized

分子量

calculated mol wt ( May vary depending on the histidine-phosphorylated proteins.)
observed mol wt ~45 kDa

种属反应性

human

包装

antibody small pack of 25 μL

环保替代产品特性

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

增强验证

recombinant expression
Learn more about Antibody Enhanced Validation

技术

western blot: suitable using 1:1,000

同位素/亚型

IgG

环保替代产品分类

运输

ambient

储存温度

2-8°C

靶向翻译后修饰

phosphorylation (N1-pHis)

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此商品
ZRB1352ZRB1002ZRB5320
biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

biological source

rabbit (recombinant)

clone

SC1-1, monoclonal, recombinant monoclonal

clone

SC56-2, monoclonal, recombinant monoclonal

clone

3K6, recombinant monoclonal

clone

1L2, monoclonal, recombinant monoclonal

species reactivity

human

species reactivity

human

species reactivity

mouse, human

species reactivity

mouse, rat

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

technique(s)

western blot: suitable using 1:1,000

technique(s)

western blot: suitable using 1:1,000

technique(s)

immunocytochemistry: suitable using 1:100, immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable using 1:100, western blot: suitable using 1:1,000

technique(s)

immunocytochemistry: suitable using 1:100, immunofluorescence: suitable using 1:100, western blot: suitable using 1:1,000

一般描述

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb antibodies represent an entirely new generation of recombinant monoclonal antibodies.

Each ZooMAb antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb antibodies are reliably available and ready to ship when you need them.

Learn more about ZooMAb here.

特异性

Clone SC1-1 is a ZooMAb rabbit recombinant monoclonal antibody that selectively detects proteins with histidine(s) phosphorylated at N1 of the imidazole ring (1-pHis), but not 3-pHis.

免疫原

KLH-conjugated library of random peptides containing non-hydrolyzable phosphohistidine analogue 1-pTza.

应用

Anti-N1-Phosphohistidine (1-pHis), clone SC1-1 ZooMAb, Cat. No. ZRB1330, is a recombinant Rabbit Monoclonal Antibody that targets N1-Phosphohistidine and has been tested for use in Western Blotting.

目标描述

Phosphorylation plays an important role in regulating protein activities and various cellular signaling events in cells. Limited by the tools available for phosphohistidine (pHis) detection, the majority of studies focus on serine, threonine, and tyrosine phosphorylations. Histidine phosphorylation can occur at either N1 (1-pHis) or N3 (3-pHis) of the imidazole ring. The development of peptides containing stable phosphoryltriazolylalanine analogues of 1-pHis and 3-pHis (1-pTza and 3-pTza) allows the generation of antibodies for studying both histidine N1 and N3 phosphorylations in signaling events. There is growing evidence implicating His kinases in cancer and tumor metastasis and the first metastasis suppressor gene identified is one of the two known mammalian His kinases, Nm23-H1 (also known as NME1, nucleoside diphosphate kinase, or NDPK-A). Nm23-H1/NME1 and the closely related Nm23-H2 (NME2/NDPK-B) catalyze the transfer of phosphate from ATP onto Nucleoside-diphosphates (NDPs) through a 1-pHis enzyme intermediate. Nm23-H1/-H2 also possess His kinase activity, transferring the phosphate from the active site pHis onto a His in a target protein. Metabolic enzymes such as phosphoglycerate mutase (PGAM), succinyl CoA synthase (SCS), and ATP citrate lyase (ACL) also use pHis as an enzyme intermediate. Unlike NME1/2, PGAM uses 3-pHis as an enzyme intermediate. In addition to eukaryotes, histidine phosphorylation is well documented in bacterial two-component signaling pathways involved in chemotaxis, although the phosphate is transferred from the pHis formed in the receptor/sensor protein to Asp residues of an acceptor response regulator protein, and the receptor/sensor protein essentially functions as an aspartate kinase. This ZooMAb recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals.

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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Jane E Schulte et al.
The Journal of biological chemistry, 296, 100090-100090 (2020-11-18)
Histidine phosphorylation is a posttranslational modification that alters protein function and also serves as an intermediate of phosphoryl transfer. Although phosphohistidine is relatively unstable, enzymatic dephosphorylation of this residue is apparently needed in some contexts, since both prokaryotic and eukaryotic

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