Merck

ZRB1388

Sigma-Aldrich

Anti-Elastin Antibody, clone 1G6 ZooMAb® Rabbit Monoclonal

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

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别名:
Tropoelastin

生物来源

rabbit

质量水平

重组

expressed in HEK 293 cells

偶联物

unconjugated

抗体形式

purified antibody

antibody product type

primary antibodies

克隆

1G6, recombinant monoclonal

描述

recombinant, expressed in HEK 293 cells

产品线

ZooMAb® learn more

形式

lyophilized

分子量

calculated mol wt 68.4 kDa
observed mol wt ~100 kDa

纯化方式

using Protein A

species reactivity

human

包装

antibody small pack of 25 μL

环保替代产品特性

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

增强验证

recombinant expression
Learn more about Antibody Enhanced Validation

technique(s)

immunofluorescence: suitable
immunohistochemistry: suitable
western blot: suitable

同位素/亚型

IgG

表位序列

C-terminal half

Protein ID登记号

UniProt登记号

环保替代产品分类

运输

ambient

储存温度

2-8°C

target post-translational modification

unmodified

Gene Information

human ... ELN(2006)

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clone

1G6, recombinant monoclonal

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1G2, recombinant monoclonal

clone

1F6, recombinant monoclonal

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1G5, monoclonal, recombinant monoclonal

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ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

product line

ZooMAb® learn more

一般描述

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1G6 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Elastin. It targets an epitope within 17 amino acids from the C-terminal half.

免疫原

KLH-conjugated linear peptide corresponding to 17 amino acids from the C-terminal half of human Elastin.

应用

Quality Control Testing

Evaluated by Western Blotting in Human lung tissue lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Elastin in Human lung tissue lysate.

Tested Applications

Immunofluorescence Analysis: A 1:100 dilution from a representative lot detected Elastin in human lung tissue sections.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Elastin in human lung tissue sections.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

目标描述

Elastin (UniProt: P15502; also known as Tropoelastin) is encoded by the ELN gene (Gene ID: 2006) in human. Elastin is a connective tissue protein found in the extracellular matrix of most vertebrate tissues and is an important part in the interstitium of tissues. it is also a component of ECM proteins involved in the tumor microenvironment where it is reported to modulate the cellular physiology of tumor cells, stromal fibroblasts, smooth muscle cell, endothelial cells, and inflammatory cells. It has an extensive crosslinked structures that imparts a long half-life to this protein. Due to its polymeric nature, it resists acid and alkali treatment. It is about 1000-fold more flexible than collagens; thus, its main function is to provide elasticity to tissues. Elastin is synthesized with a signal peptide (aa 1-26), which is subsequently cleaved off to produce mature protein (aa 27-786). Its expression is observed in large arteries, heart valves, pulmonary tissue, and certain ligaments and cartilages. It is an essential component of tissues that undergo rapid expansion and recovery. Elastin is formed through cross-linking of tropoelastin, the precursor form of elastin. The cross-linking is initiated through the action of lysyl oxidase on exposed lysines to form allysine. The most abundant cross-links in mature elastin fibers are lysinonorleucine, allysine aldol, desmosine, and isodesmosine. Tropoelastin contains two major domains: alternating hydrophobic regions and hydrophilic cross-link domains. The hydrophobic domains are reported to be rich in non-polar amino acids (glycine, valine, proline, and alanine) that appear in repeats of 3 to 6 peptides. The hydrophilic cross-link domains are rich in alanine and lysine and are required for the lysyl oxidase-mediated formation of desmosine cross-links. These are required for the formation of insoluble elastin. Thirteen different isoforms of elastin have been described that are produced by alternative splicing. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Wang, K., et a; (2021). Front. Cell Dev. Biol. 9; 596702; Li; J., et al. (2020). BMC Cancer. 20(1); Article 217).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


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