Merck

ZRB1845

Sigma-Aldrich

Anti-Progesterone Receptor Antibody, clone 1D2 ZooMAb® Rabbit

enhanced validationgreener alternative

recombinant, expressed in HEK 293 cells

登录查看公司和协议定价

别名:
PR, Nuclear receptor subfamily 3 group C member 3

生物来源

rabbit

质量水平

重组

expressed in HEK 293 cells

偶联物

unconjugated

抗体形式

purified antibody

抗体产品类型

primary antibodies

克隆

1D2, recombinant monoclonal

描述

1D2 Clone

产品线

ZooMAb® learn more

形式

lyophilized

分子量

calculated mol wt 82.29 kDa
observed mol wt ~110 and 80 kDa

纯化方式

using Protein A

种属反应性

human

种属反应性(根据同源性预测)

rat, monkey, mouse

包装

antibody small pack of 25 μL

环保替代产品特性

Waste Prevention
Designing Safer Chemicals
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

增强验证

recombinant expression
Learn more about Antibody Enhanced Validation

技术

affinity binding assay: suitable
immunocytochemistry: suitable
immunohistochemistry: suitable
western blot: suitable

同位素/亚型

IgG

表位序列

Internal

Protein ID登记号

UniProt登记号

环保替代产品分类

运输

ambient

储存温度

2-8°C

靶向翻译后修饰

unmodified

基因信息

human ... PGR(5241)

一般描述

We are committed to bringing you greener alternative products, which adhere to one or more of The 12 Principles of Green Chemistry.This antibody is Preservative-free, produced without the harm or sacrifice of animals and exceptionally stable to allow for ambient shipping and storage if needed and thus aligns with "Waste Prevention", "Designing Safer Chemicals" and "Design for Energy Efficiency". Click here for more information.
ZooMAb® antibodies represent an entirely new generation of recombinant monoclonal antibodies.Each ZooMAb® antibody is manufactured using our proprietary recombinant expression system, purified to homogeneity, and precisely dispensed to produce robust and highly reproducible lot-to-lot consistency. Only top-performing clones are released for use by researchers. Each antibody is validated for high specificity and affinity across multiple applications, including its most commonly used application. ZooMAb® antibodies are reliably available and ready to ship when you need them.

特异性

Clone 1D2 is a ZooMAb® Rabbit recombinant monoclonal antibody that specifically detects Progesterone receptor. It targets an epitope within 21 amino acids from the internal region. Detects isoforms 1 and 2.

免疫原

KLH-conjugated linear peptide corresponding to 21 amino acids from the internal region of human Progesterone receptor.

应用

Quality Control Testing

Evaluated by Western Blotting in T47D cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected Progesterone Receptor in T47D cell lysate.

Tested Applications

Immunocytochemistry Analysis: A 1:1,000 dilution from a representative lot detected Progesterone Receptor in MCF-7 cells.

Immunohistochemistry (Paraffin) Analysis: A 1:100 dilution from a representative lot detected Progesterone Receptor in Human fallopian tube tissue sections.

Affinity Binding Assay: A representative lot of this antibody bound Progesterone Receptor peptide A-B with a KD of 1.0 x 10-12 in an affinity binding assay.

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

目标描述

Progesterone receptor (UniProt: P06401; also known as PR, Nuclear receptor subfamily 3 group C member 3) is encoded by the PGR (also known as PR, NR3C3) gene (Gene ID: 5241) in human. Progesterone receptor, a member of the superfamily of steroid receptors, mediates the action of progesterone on its target tissues. Two major isoforms of progesterone receptor, known as progesterone receptor A (PR-A) and PR-B, have been described. They are structurally related, but functionally distinct proteins, produced by transcription from alternative estrogen-inducible promoters and alternate translational sites within a single PR gene. Depending on the isoform, progesterone receptor may function as transcriptional activator or repressor. In reproductive tissues the expression of isoform A and B varies as a consequence of developmental and hormonal status. Both isoforms are expressed in comparable amounts in the uterine glandular epithelium during the proliferative phase of the menstrual cycle. However, the expression of isoform B persists in the glands during mid-secretory phase. In the stroma, isoform A is the predominant form throughout the cycle. Progesterone receptor is phosphorylated on multiple serine residues in a hormone-dependent manner. Phosphorylation on serine 294 that occurs preferentially on isoform B is highly hormone-dependent and modulates ubiquitination and sumoylation on lysine 388. Phosphorylation on serine 102 and 345 is also hormone dependent. Basal phosphorylation on serine residues 81, 162, 190, and 400 is also increased in response to progesterone and can be phosphorylated in vitro by the Cdk2-A1 complex. Phosphorylation at serine 162 and 294, but not at 190, is impaired during the G2/M phase of the cell cycle. This ZooMAb® recombinant monoclonal antibody, generated by our propriety technology, offers significantly enhanced specificity, affinity, reproducibility, and stability over conventional monoclonals. (Ref.: Pierson-Mullany, LK., and Lange, CA. (2004). Mol. Cell Biol. 24(24); 10542-10557; Clemm, DL., et al. (2000). Mol. Endocrinol. 14(1); 52-65; Giangrande, PH., et al. (2000). Mol. Cell Biol. 20(9); 3102-3115).

外形

Purified recombinant rabbit monoclonal antibody IgG, lyophilized in PBS with 5% Trehalose, normal appearance a coarse or translucent resin. The PBS/trehalose components in the ZooMAb formulation can have the appearance of a semi-solid (bead like gel) after lyophilization. This is a normal phenomenon. Please follow the recommended reconstitution procedure in the data sheet to dissolve the semi-solid, bead-like, gel-appearing material. The resulting antibody solution is completely stable and functional as proven by full functional testing. Contains no biocide or preservatives, such as azide, or any animal by-products. Larger pack sizes provided as multiples of 25 μL.

重悬

300 μg/mL after reconstitution at 25 μL per vial. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

储存及稳定性

Recommend storage of lyophilized product at 2-8°C; Before reconstitution, micro-centrifuge vials briefly to spin down material to bottom of the vial; Reconstitute each vial by adding 25 μL of filtered lab grade water or PBS; Reconstituted antibodies can be stored at 2-8°C, or -20°C for long term storage. Avoid repeated freeze-thaws.

法律信息

ZooMAb is a registered trademark of Merck KGaA, Darmstadt, Germany

免责声明

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存分类代码

11 - Combustible Solids

WGK

WGK 1

闪点(°F)

Not applicable

闪点(°C)

Not applicable


分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ (Lot或Batch)字后找到。

已有该产品?

为方便起见,与您过往购买产品相关的文件已保存在文档库中。

访问文档库

难以找到您所需的产品或批次号码?

在网站页面上,产品编号会附带包装尺寸/数量一起显示(例如:T1503-25G)。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题?欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ (Lot或Batch)字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等,请输入去除前两位字母的批号:09019TO。

  • 如果您查询到的批号含有填充代码(例如05427ES-021),请输入去除填充代码-021的批号:05427ES。

  • 如果您查询到的批号含有填充代码(例如 STBB0728K9),请输入去除填充代码K9的批号:STBB0728。

未找到您寻找的产品?

部分情况下,可能未在线提供COA。如果搜索不到COA,可在线索取。

索取COA

我们的科学家团队拥有各种研究领域经验,包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门