Ascentis® Express OH5, 5 μm HPLC Column

5 μm particle size, L × I.D. 3 cm × 3 mm


Quality Level


suitable for USP L86


endcapped: no




60 °C temp. range
600 bar max. pressure (9000 psi)


HPLC: suitable

L × I.D.

3 cm × 3 mm


<5 ppm metals


Fused-Core particle platform

matrix active group

hydroxy, penta- phase

particle size

5 μm

pore size

90 Å pore size

operating pH range

2 - 9

Featured Industry

Food and Beverages

separation technique

hydrophilic interaction (HILIC)

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General description

Ascentis Express 5 μm OH5 is a high-speed, high-performance liquid chromatography column based on a 5-micron Fused-Core® particle design. The Fused-Core particle provides a thin porous shell of high-purity silica surrounding a solid silica core. This particle design exhibits very high column efficiency due to the shorter diffusion paths in the 0.6-micron thick porous shell and an overall particle size of ~5-microns. The Ascentis Express 5 μm OH5 stationary phase is a highly polar ligand that possesses 5 hydroxyl groups tethered to the silica via novel proprietary linkage chemistry. This high performance material provides a column that can be used for traditional normal phase separations using non-polar, totally organic mobile phases or for aqueous normal phase chromatography with the typical mobile phases for hydrophilic interactive liquid chromatography (HILIC) of basic, acidic, zwitterionic, or neutral compounds.

Column Characteristics
The Fused-Core particle has a surface area of ~ 100 m2/g and an average pore size of 90Å. The Fused-Core particles are 30% to 50% heavier than commercially available totally porous particles due to the density of the solid cores. Therefore, the effective surface area per column is similar to columns packed with totally porous particles having surface areas in the 160-200 m2/g range.

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Legal Information

Ascentis is a registered trademark of Sigma-Aldrich Co. LLC
Fused-Core is a registered trademark of Advanced Materials Technology, Inc.


NONH for all modes of transport

WGK Germany


  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  5. My question is not addressed here, how can I contact Technical Service for assistance?

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Paweł Kubica et al.
Analytical and bioanalytical chemistry, 406(13), 3013-3018 (2014-03-26)
The influence of sucrose combustion products on smoking and nicotine addiction is still controversial because the presence of the sucrose may be treated as a source of aldehydes and organic acids. In e-liquids used as refills for electronic cigarettes, which...




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