Product Name
Discovery ® HS F5 HPLC 色谱柱, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
材料
stainless steel column
agency
suitable for USP L43
產品線
Discovery®
特點
endcapped
製造商／商標名
Discovery®
包裝
1 ea of
標籤範圍
12% Carbon loading
參數
≤70 °C temp. range
400 bar pressure (5801 psi)
技術
HPLC: suitable
LC/MS: suitable
長度 × 內徑
5 cm × 4.6 mm
表面積
300 m2/g
表面覆盖率
4 μmol/m2
雜質
<10 ppm metals
基質
silica gel, high purity, spherical particle platform
fully porous particle
基質活性組
PFP (pentafluorophenyl) phase
粒徑
5 μm
孔徑
120 Å
工作pH值範圍
2-8
應用
food and beverages
分離技術
reversed phase
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一般說明
将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则：
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来，使得大部分的 C18 方法可轻松转换到 F5 上。
應用
- Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring.: This study utilizes the Discovery® HS F5 HPLC Column to develop an HPLC-UV method for the simultaneous determination of phenylalanine and tyrosine in blood, significantly advancing the diagnostic and monitoring capabilities for phenylketonuria (Rabti et al., 2024).
- A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities.: Demonstrates the application of the Discovery® HS F5 HPLC Column in optimizing sample preparation techniques for the detection of pharmaceuticals and personal care products in environmental samples, enhancing the analysis of diverse hydrophilic compounds (Portillo-Castillo et al., 2021).
- "Heart-cut" bidimensional achiral-chiral liquid chromatography applied to the evaluation of stereoselective metabolism, in vivo biological activity and brain response to chiral drug candidates targeting the central nervous system.: Utilizes the Discovery® HS F5 HPLC Column in a novel bidimensional chromatographic setup to study the stereoselective metabolism of CNS-targeting chiral drugs, contributing to a deeper understanding of their pharmacological profiles (Battisti et al., 2016).
- HPLC method development, validation, and impurity characterization of a potent antitumor indenoisoquinoline, LMP776 (NSC 725776).: This research employs the Discovery® HS F5 HPLC Column for developing an HPLC method to identify and characterize impurities in the antitumor drug LMP776, ensuring drug purity and compliance with regulatory standards (Wang et al., 2016).
- Development studies on determination of preservatives decomposition products.: Showcases the application of the Discovery® HS F5 HPLC Column in the analysis of decomposition products of preservatives, enhancing the safety assessments of food and pharmaceutical products (Zabrzewska et al., 2014).
特點和優勢
- 独特的选择性
- 保留能力与 C18 柱类似（有时需要洗脱能力更强的流动相）
- 峰形极佳
- 稳定性好、低流失，适用于 LC-MS 分析
- 粒径范围从 3μm 到 10μm 可供选择
法律資訊
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
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