An overview of the principles and applications of Western blotting for protein detection. Includes links to detailed protocols, videos, and other resources for protein extraction, gel electrophoresis, transfer to PVDF or nitrocellulose membranes, and chemiluminescent, colorimetric, and fluorescent detection methods.
Immobilon® GO simplifies the immunodetection process by eliminating tedious, manual steps. Simply load the Immobilon® GO device with your blot, blocking buffer, wash buffer, and primary and secondary antibody solutions. Once setup is complete, no manual intervention is required—and you are free to focus on other tasks.