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Internal amino acid sequence analysis of proteins separated by one- or two-dimensional gel electrophoresis after in situ protease digestion on nitrocellulose.
Aebersold RH
Proceedings of the National Academy of Sciences of the USA, 84(20), 6970-6974 (1987)
Tomáš Dráb et al.
Journal of separation science, 34(14), 1692-1695 (2011-06-18)
Native polyacrylamide electrophoresis in the presence of two reversible protein anionic stains (Ponceau S and Ponceau 2R) was used to study the oligomeric states of soluble proteins. A mild binding of the used protein stains to nondissociated protein oligomers imposed
Trifluoroacetylated proteins in liver and plasma of guinea pigs treated with HCFC-123 and halothane.
Silvia Bortolato et al.
Toxicology letters, 144(1), 35-47 (2003-08-16)
Trifluoroacetylated (TFA)-protein adducts were investigated by immunoblotting in liver and plasma of guinea pigs treated with the hepatotoxic anaesthetic halothane or the chlorofluorocarbon replacement 1,1-dichloro-2,2,2-trifluoroethane (HCFC-123). Male outbred Hartley guinea pigs (320-400 g) were administered HCFC-123 (1, 5, 10 and
Sarah Nicklas et al.
PloS one, 7(1), e30445-e30445 (2012-02-03)
Limb girdle muscular dystrophy type 2H (LGMD2H) is an inherited autosomal recessive disease of skeletal muscle caused by a mutation in the TRIM32 gene. Currently its pathogenesis is entirely unclear. Typically the regeneration process of adult skeletal muscle during growth
Sue Ellen Touma et al.
Biochemical pharmacology, 78(9), 1127-1138 (2009-06-25)
Retinoids, which include vitamin A (retinol) and metabolites such as retinoic acid, can inhibit tumor growth and reverse carcinogenesis in animal models of prostate cancer. We analyzed retinoid signaling and metabolism in the TRAMP (transgenic adenocarcinoma mouse prostate) model. We
Yumiko Yasui et al.
Biological & pharmaceutical bulletin, 30(3), 443-446 (2007-03-03)
Heme oxygenase (HO)-1, which is a rate-limiting enzyme involved in the catabolism of heme, is upregulated by a variety of stresses including oxidative stresses and inflammatory cytokines, in many cell types. Recent studies have suggested that upregulation of HO-1 might
T G van Kooten et al.
Journal of biomedical materials research, 54(3), 385-389 (2001-02-24)
Research of cell-biomaterial interactions is building on knowledge and methods available in cell and molecular biology. Western blotting is one of the options to characterize protein expression in cell populations. Method transfer to biomaterial model systems is not trivial because
Laura Wilding Crawford et al.
PloS one, 3(2), e1611-e1611 (2008-02-26)
In the past decade, several transcription factors critical for pancreas organogenesis have been identified. Despite this success, many of the factors necessary for proper islet morphogenesis and function remain uncharacterized. Previous studies have shown that transgenic over-expression of the transcription
Hong-Wen Gao et al.
Biophysical journal, 94(3), 906-917 (2007-10-02)
Using various methods, e.g., spectrophotometry, circular dichroism, and isothermal titration calorimetry, the interaction of poncean S (PS) with human serum albumin (HSA) was characterized at pH 1.81, 3.56, and 7.40 using the spectral correction technique, and Langmuir and Temkin isothermal
Maoyong Fu et al.
PloS one, 6(5), e19945-e19945 (2011-06-04)
Endometrial cancer is the most common gynecologic malignancy diagnosed among women in developed countries. One recent biomarker strongly associated with disease progression and survival is epithelial membrane protein-2 (EMP2), a tetraspan protein known to associate with and modify surface expression
Herb Francisco et al.
Developmental neurobiology, 69(2-3), 162-173 (2008-12-17)
Many neuronal cytosolic and nuclear proteins are post-translationally modified by the reversible addition of O-linked N-acetylglucosamine (O-GlcNAc) on serines and threonines. The cellular functions of O-GlcNAc modifications in neuronal development are not known. We report that O-GlcNAc-modified proteins are distributed
Sean Gallagher et al.
Current protocols in molecular biology, Chapter 10, Unit 10-Unit 10 (2008-02-12)
Immunoblotting (western blotting) is used to identify specific antigens recognized by polyclonal or monoclonal antibodies. This unit provides protocols for all steps starting with solubilization of the protein samples, usually with SDS and reducing agents. Following solubilization, the material is
Christine Blancher et al.
Methods in molecular biology (Clifton, N.J.), 878, 89-110 (2012-06-08)
The study of proteins, their expression and post-translational modification, is a key process in molecular biology. Immunoblotting is a well-established and powerful tool for the study of proteins, which continues to evolve as new reagents and apparatus are developed. This
Pedro Rodriguez-Collazo et al.
Nucleic acids research, 37(11), e81-e81 (2009-05-16)
Post-translational modifications (PTMs) of histones play a role in modifying chromatin structure for DNA-templated processes in the eukaryotic nucleus, such as transcription, replication, recombination and repair; thus, histone PTMs are considered major players in the epigenetic control of these processes.
High-efficiency staining of proteins on different blot membranes.
C R Yonan et al.
Analytical biochemistry, 338(1), 159-161 (2005-02-15)
S Harper et al.
Current protocols in protein science, Chapter 10, Unit 10-Unit 10 (2008-04-23)
Staining of blot transfer membranes permits visualization of proteins and allows the extent of transfer to be monitored. In the protocols described in this unit, proteins are stained after electroblotting from one-dimensional or two-dimensional polyacrylamide gels to blot membranes such
Mari-Cruz Castillo et al.
Science signaling, 8(392), ra89-ra89 (2015-09-04)
Abscisic acid (ABA) is a phytohormone that inhibits growth and enhances adaptation to stress in plants. ABA perception and signaling rely on its binding to receptors of the pyrabactin resistance1/PYR1-like/regulatory components of ABA receptors (PYR/PYL/RCAR) family, the subsequent inhibition of
Els Pattyn et al.
PloS one, 3(6), e2427-e2427 (2008-06-19)
ISG15 is an Ubiquitin-like protein, highly induced by Type I Interferons. Upon the cooperative activity of specific Ubiquitinating enzymes, ISG15 can be conjugated to its substrates. Increasing evidence points to a role for protein ISGylation in anti-viral and anti-tumoral defense.
Joris Wauman et al.
Journal of cell science, 124(Pt 6), 921-932 (2011-03-08)
Cytokines, such as interferons, erythropoietin, leptin and most interleukins, signal through type 1 cytokine receptors and activate the canonical JAK-STAT pathway. Aberrant cytokine signalling underlies numerous pathologies and adequate, temporary receptor activation is therefore under tight control. Negative-feedback mechanisms are
Samantha G Zeitlin et al.
PloS one, 6(3), e17151-e17151 (2011-03-15)
Uracil is removed from DNA by the conserved enzyme uracil DNA N-glycosylase (UNG). Previously, we observed that inhibiting UNG in Xenopus egg extracts blocked assembly of CENP-A, a histone H3 variant. CENP-A is an essential protein in all species, since
Elisabete Silva et al.
Biology of reproduction, 93(3), 56-56 (2015-07-17)
The number of women who delay their first childbirth is increasing. This demographic shift is an important health issue because advanced maternal age is a risk factor for reproductive capacity loss and the occurrence of placental bed disorders that may
Guihong Zhang et al.
BMC cancer, 11, 32-32 (2011-01-27)
Retinoid receptor-induced gene-1 (RRIG1) is a novel gene that has been lost in several types of human cancers. The aim of this study was to determine whether RRIG1 plays a role in breast cancer, such as in the suppression of
Expression of cyclooxygenase-2 (COX-2) in human invasive transitional cell carcinoma (TCC) of the urinary bladder.
Mohammed SI
Cancer Research, 59(22), 5647-5650 (1999)
Todd R Heallen et al.
Developmental cell, 15(4), 603-616 (2008-10-16)
The Aurora B kinase is the enzymatic core of the chromosomal passenger complex, which is a critical regulator of mitosis. To identify novel regulators of Aurora B, we performed a genome-wide screen for suppressors of a temperature-sensitive lethal allele of
Orsolya Polgar et al.
Molecular and cellular biochemistry, 322(1-2), 63-71 (2008-11-13)
ABCG2 is a half-transporter initially described in multidrug-resistant cancer cells and lately identified as an important factor in the pharmacokinetics of its substrates. Q141K is by far the most intensively studied single nucleotide polymorphism of ABCG2 with potential clinical relevance.
Hanan Hafez Omar
Pakistan journal of biological sciences : PJBS, 11(10), 1310-1316 (2008-09-27)
This study is to inspect how the variation of molecular structures and functional groups present in our models, monoazo dye (Tartrazine) and diazo dye (Ponceau), affects decolorization capabilities of green algae, cyanobacteria and diatoms. The results revealed that the removal
Tomáš Dráb et al.
Electrophoresis, 32(24), 3597-3599 (2011-12-20)
A new type of native electrophoresis was developed to separate and characterize proteins. In this modification of the native blue electrophoresis, the dye Ponceau Red S is used instead of Coomassie Brilliant Blue to impose uniform negative charge on proteins
Youji Shimazaki et al.
Protein and peptide letters, 17(8), 1048-1052 (2010-04-17)
Mouse liver cytosolic malate dehydrogenase was separated by non-denaturing two-dimensional electrophoresis and identified. Furthermore, the activity of the enzyme was preserved even after separation, electroblotting onto a membrane and staining with Ponceau S in acidic buffer solution (pH 5.1). Using
Benjamin N Philip et al.
The Journal of experimental biology, 211(Pt 7), 1114-1119 (2008-03-18)
Survival of freezing not only requires organisms to tolerate ice formation within their body, but also depends on the rapid redistribution of water and cryoprotective compounds between intra- and extracellular compartments. Aquaporins are transmembrane proteins that serve as the major
P Grundtner et al.
Genes and immunity, 10(1), 84-92 (2008-09-19)
Genetic predisposition is a risk factor for the development of inflammatory bowel diseases (IBDs). Disruption of the interleukin (IL)-10 pathway in mice causes intestinal inflammation similar to human IBD. Two common non-synonymous IL-10R1 variants, S138G and G330R, were cloned and
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