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磷酸钙转染试剂盒实验方案

使用磷酸钙将DNA转入真核细胞是一种常见的转染方法。该转染实验方案可经优化用于各种细胞类型。

坚固耐用且可重现的固相微萃取（SPME）采样

现在，我们推出了同样可采用Carboxen®/聚二甲基硅氧烷（CAR/PDMS）或聚二甲基硅氧烷/二乙烯基苯（PDMS/DVB）涂层的SS组件形式的镍钛合金（NIT）内核SPME萃取头。

DNA/蛋白质电泳及故障排除表

DNA和蛋白质凝胶电泳(SDS-PAGE)应用中的疑难问题解答。提供琼脂糖凝胶中DNA的有效分离范围和加样染料的DNA迁移大小。

顶空级溶剂在有机挥发性杂质分析之中的应用

使用Jumpstart™Taq DNA聚合酶(D9307)扩增DNA

使用抗体介导热启动聚合酶的实验方案。一种具有短活化阶段的方法（<1min), and results in higher yields and more specificity over standard PCR methods.

FITC标记多糖

包括FITC-葡聚糖、FITC-DEAE-葡聚糖(FDD)、FITC-羧甲基 - 葡聚糖（FCM-葡聚糖）在内的FITC标记多糖的补充信息。本页面主要介绍了重均分子量对于对应分子尺寸和其他物理性质的影响

胰蛋白酶抑制剂

天然胰蛋白酶抑制剂也称为丝氨酸蛋白酶抑制剂（serpins），是最大和最多样化的蛋白酶抑制剂家族。Serpins通过抑制体内丝氨酸蛋白酶来控制蛋白质的活化和分解代谢。

适用于高效稳定的染料敏化太阳能电池的有机染料

在过去的十年中，染料敏化太阳能电池 (DSSC) 备受关注，因为此类非常规太阳能电池不仅性能出众，而且还具有低成本生产的潜力。

氮化硼纳米管：性能、合成和应用

氮化硼纳米管（BNNT）是碳纳米管（CNT）的结构类似物，而碳纳米管是高纵横比纳米管材料，其中碳原子被氮原子和硼原子交替取代。

Lipopolysaccharides

Lipopolysaccharides (LPS) are characteristic components of the cell wall of Gram negative bacteria; they are not found in Gram positive bacteria. They are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell

人前脂肪细胞(HPAd)培养方案

将细胞放入冻存管后须立即储存在液氮储罐中。

3D类器官培养：用于开发及疾病研究的新体外模型

可生成组织和干细胞来源3D脑、胃肠道、肺和癌症肿瘤类器官模型的类器官培养产品。

杂化钙钛矿太阳能电池最新进展

近几十年来，人们对于环境可持续、商业可行的能源的迫切需求，催生并推动了大量致力实现低生产成本、高能效发电系统的研究工作。

Separation Options with Superose®

Separation options for Size Exclusion Chromatography with Superose from Cytiva.

Separation Options with Superdex®

Superdex® from Cytiva are Size Exclusion Chromatography media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a Superdex® column.

Sepharose® High Performance: Purification with High Resolution

Here we discuss purification options when working with Sepharose® High Performance and we present some examples of media screening, capture and purification.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Detection of GST-tagged Proteins with Western Blot

This page describes how to monitor expression and purification of GST-tagged proteins by Western blot analysis using Cytiva products.

Cleavage and Purification of GST-Tagged Protein Bound to Glutathione Sepharose in Batch Mode

This page shows how to cleave and purify GST-tagged proteins bound to Glutathione Sepharose in batch mode from Cytiva. Glutathione Sepharose High Performance, Glutathione Sepharose 4 Fast Flow, and Glutathione Sepharose 4B can all be used for cleavage and purification

Performing a Purity and Homogeneity Check

This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.

Puriﬁcation of Histidine-Tagged Proteins Secreted into Eukaryotic Cell Culture Supernatants Using Ni Sepharose® Excel

This page shows how to purify histidine-tagged proteins secreted into eukaryotic cell culture supernatants using Ni Sepharose Excel from Cytiva.

Troubleshooting of Cleavage Methods

This page shows troubleshooting strategies for cleavage methods using Cytiva products.

DNA / Protein Electrophoresis and Troubleshooting Tables

radiello® Diffusive Air Sampling Application - Anaesthetic Gases and Vapors

Protocol for radiello® Diffusive Air Sampling Application - Anaesthetic Gases and Vapors

Sepharose High Performance: Purification with High Resolution

This page covers the use of Sepharose High Performance media for purification of proteins, peptides or oligonucleotides, when to use them, and with which systems.

Enzymatic Assay of Aprotinin

Objective: To standardize a procedure for the enzymatic assay of Aprotinin.

Imprint® Methylated DNA Quantification Kit (MDQ1) Protocol

The Imprint® Methylated DNA Quantification Kit provides a high-throughput, non-radioactive means of measuring global DNA methylation shifts.

Metal Borohydrides as Hydrogen Storage Materials

An article about metal borohydrides as hydrogen storage materials

Enzymatic Assay of Esterase

Objective: To standardize a procedure for the enzymatic determination of Esterase activity using Ethyl Butyrate as a substrate.

Analysis of Fluorescent Dyes Used for Labeling DNA in Microarray Experiments

An article concerning Analyzing Properties of Fluorescent Dyes for Labeling DNA in Microarray Experiments presented by Sigma-Aldrich.com

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