文件类型
显示 1-30 共 101 条结果 关于 "200-370-5"
3D Biotek Cell Seeding Flow-Chart
3h (μl) PS1520 PS3040 PCL3030 PCL3050 96-well 185 180 180 175 48-well 225 200 200 180 24-well 440 380 370 340 12-well 1380 1260 1230 1160 6-well 1700 1400 1310 1120
Data Sheet - T8665
substrate produces a soluble end product that is blue in color and can be read spectrophotometrically at 370 or 655 nm. The reaction may be stopped with acid, resulting in a yellow solution that is read at
3D Insert Cell Seeding Protocol
PS3040  PCL3030  PCL3050 96‐well   185  180  180  175  200  200  200  200  48‐well   225  200  200  180  250  250  250  250  24‐well   440  380  370  340  500  500  500  500  12‐well   1380  1260  1230
Product Information Sheet - MAK169
for each reaction (well). Table 1. Master Reaction Mix Reagent Volume 200× PPi Sensor stock solution 25 µL Assay Buffer 5 mL Note: The Master Reaction Mix is enough for one plate and can be scaled
Product Information Sheet - E2642
with 200 µL PBS. Repeat the washing 3-5 times. 4. Apply the sample containing the biotinylated protein/antibody at ~750 µg/test. Incubate for 30 min at RT using a rotating platform. 5. Wash
Data Sheet - T3405
suitable for use in ELISA procedures. 1-5 This substrate produces a soluble end product that is blue in color and can be read spectrophotometrically at 370 or 655 nm. The reaction may be stopped
Data Sheet - T5525
horseradish peroxidase conjugates. 1-5 This substrate produces a soluble end product that is blue in color and can be read spectrophotometrically at 370 or 655 nm. The reaction maybe stopped
Product Information Sheet - 11828789001
solution 4 5× 300 �l 5× 1 min 3 Add 200 �l of solution 5 to MP wells 200 �l approx. 10 –20 min at RT 300 rpm on a shaker 4a Alternative without stop reagent:...
Data Sheet - S5187
. et al., Nature, 362, 318 (1993). 4. Ferro-Novick, S. and Jahn, R., Nature, 370, 191 (1994). 5. Sudhof, T., Nature, 375, 645 (1995). 6. Linial, M., J. Neurochem., 69...
Product Information Sheet - MAK109
each dilution to a fluorescent compatible microplate. Measure the fluorescence intensity (ex = 370/em = 450 nm). 0.2 mL of LPL Assay Buffer is used as the 0 (Blank) Standard (see Table 1.).
Data Sheet - P7243
3. Bond, C.T. et al., Ann. N.Y. Acad. Sci., 868, 370 (1999). 4. Grosse, G. et al., J. Neurosci., 20, 1869 (2000). 5. Teschemacher, A.G. et al., Br. J. Pharmacol., 128, 479 (1999
Data Sheet - X3378
PAGE, visualized by silver stain. References 1. Wagenknecht, B., et al., Cell Death Differ., 6, 370- 376 (1999). 2. Sun, C., et al., Nature, 401, 818-822 (1999). 3. Roy, N., et al., EMBO J., 16
EX-CELL 420 Serum-Free Medium for Insect Cells
DMSO). 3. Centrifuge the cells at 200 g for 5 minutes. Remove the supernatant. 4. Resuspend the cells in the freezing medium at 5 x 106 to 1 x 107 cells/mL. 5. Rapidly transfer 1 - 2 mL of...
EX-CELL 420 Serum-Free Medium for Insect Cells
DMSO). 3. Centrifuge the cells at 200 g for 5 minutes. Remove the supernatant. 4. Resuspend the cells in the freezing medium at 5 x 106 to 1 x 107 cells/mL. 5. Rapidly transfer 1 - 2 mL of...
EX-CELL 420 Serum-Free Medium for Insect Cells
DMSO). 3. Centrifuge the cells at 200 g for 5 minutes. Remove the supernatant. 4. Resuspend the cells in the freezing medium at 5 x 106 to 1 x 107 cells/mL. 5. Rapidly transfer 1 - 2 mL of...
Data Sheet - P8107
rabbit using as immunogen peptide (C)KKYK YVEDY EQGLS GEMNQ corresponding to amino acid residues 370-388 of rat P2X4.1-4 The antibody is affinity isolated using peptide-agarose. Anti-P2X4 Purinergic
Anti-Caspase 1 (ICE) (C4851)
(1989). 3. Cerreti, D.P., et al., Science, 256, 97 (1992). 4. Wilson, K.P., et al., Nature, 370, 270 (1994). 5. Walker, N.P., et al., Cell, 78, 343 (1994). 6. Howard, A.D., et al., J. Immunol., 147, 2964
Data Sheet - C9602
J. C. et al., Anesth. Analg., 90, 727 (2000). 10. Bond, C. T. et al., Ann. N.Y. Acad. Sci., 868, 370 (1999). 11. Grosse, G. et al., J. Neurosci., 20, 1869 (2000). 12. McFarlane, S. and Pollock, N
Data Sheet - P9482
discarded if not used within 12 hours. Product Profile The recommended working dilution is 1:70 – 1:200 (5-15 µg/ml) for immunoblotting using an anti-rabbit IgG-peroxidase conjugate and detection by ECL.
Data Sheet - P0856
if not used within 12 hours. Product Profile The recommended working dilution is 1:70 - 1:200 (15 – 5 µg/ml) for immunoblotting using peroxidase conjugated-goat anti-rabbit IgG and detection by ECL
Data Sheet - C7597
, J.C. et al., Anesth. Analg., 90, 727 (2000). 12. Bond, C.T. et al., Ann. N.Y. Acad. Sci., 868, 370 (1999). 13. Grosse, G. et al., J. Neurosci., 20, 1869 (2000). 14. McFarlane, S. and Pollock
Product Information Sheet - 99314
intracellular ion background of 200 mM K+, 2.0 mM Mg2+, 0.001 mM Ca2+): log aNa ~-2.5 Electrical resistance, tip diameter ~2 µm: ~1010 Ω Response time: 90% response time ≤5 s Electrode Characteristics
Data Sheet - P9978
, J.C. et al., Anesth. Analg., 90, 727 (2000). 11. Bond, C.T. et al., Ann. N.Y. Acad. Sci., 868, 370 (1999). 12. Grosse, G. et al., J. Neurosci., 20, 1869 (2000). 13. McFarlane, S. and Pollock
Data Sheet - P6102
Dreixler, J.C. et al., Anesth. Analg., 90, 727 (2000). 12. Bond, C.T. et al., Ann. N.Y. Acad. Sci., 868, 370 (1999). 13. Grosse, G. et al., J. Neurosci., 20, 1869 (2000). 14. McFarlane, S., and Pollock, N.S
Data Sheet - P9857
Dreixler, J.C. et al., Anesth. Analg., 90, 727 (2000). 12. Bond, C.T. et al., Ann. N.Y. Acad. Sci., 868, 370 (1999). 13. Grosse, G. et al., J. Neurosci., 20, 1869 (2000). 14. McFarlane, S. and Pollock, N.S
Data Sheet - P4996
before use (10000 x g 5 min). Working dilution samples should be discarded if not used within 12 hours. Product Profile The recommended working dilution is 4 µg/ml (1:200) for immunoblotting.
Data Sheet - P9232
, J.C. et al., Anesth. Analg., 90, 727 (2000). 11. Bond, C.T. et al., Ann. N.Y. Acad. Sci., 868, 370 (1999). 12. Grosse, G. et al., J. Neurosci., 20, 1869 (2000). 13. McFarlane, S. and Pollock
Data Sheet - P9982
J.C. et al., Anesth. Analg., 90, 727 (2000). 11. Bond, C.T. et al., Ann. N.Y. Acad. Sci., 868, 370 (1999). 12. Grosse, G. et al., J. Neurosci., 20, 1869 (2000). 13. McFarlane, S....
Data Sheet - P4621
before use (10000 x g 5 min). Working dilution samples should be discarded if not used within 12 hours. Product Profile The recommended working dilution is 2.5 µg/ml (1:200) for immunoblotting
Data Sheet - P9357
Dreixler, J.C. et al., Anesth. Analg., 90, 727 (2000). 11. Bond, C.T. et al., Ann. N.Y. Acad. Sci., 868, 370 (1999). 12. Grosse, G. et al., J. Neurosci., 20, 1869 (2000). 13. McFarlane, S. and Pollock, N.S

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