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Product Information Sheet-tosrtro
4 Control RNA HAV RNA, in vitro transcript. 04 655 877 001 50 μl, 200 copies/μl
5 Control Primer Mix,
HAV RNA
HAV RNA upstream
and downstream primer to
amplify a 246 bp fragment.
04 655 877
Data Sheet - T8665
utilize horseradish peroxidase conjugates.1-4 This
substrate produces a soluble end product that is blue in
color and can be read spectrophotometrically at 370 or
655 nm. The reaction may be stopped with
Product Information sheet - T8665
peroxidase (HRP)
conjugates.1-4 This TMB-HRP reaction produces a
soluble end-product that is blue in color and can be
read spectrophotometrically at 370 nm or 655 nm.
The reaction may be stopped
Product Information Sheet - MAK152
enzyme assay,
which results in a colorimetric (655 nm) product
proportional to the SMase activity present.
Components
The kit is sufficient for 200 assays in 96 well plates.
Enzyme Mix 2 ea
Catalog
BioFiles-Issue
mg
05316 Atto 647N-maleimide 1 mg
93711 Atto 655 1 mg
06966 Atto 655-Biotin 1 mg
80661 Atto 655-maleimide 1 mg
76245 Atto 655-NHS ester 1 mg
02744 Atto 655-Streptavidin 1 mg
94875 Atto 680 1 mg
04971 Atto
Data Sheet - A3986
Biol., 150,
643-655 (2000).
2. Mykkanen, O.M. et al., Mol. Biol. Cell, 12, 3060-
3073 (2001).
3. Rachlin, A.S., and Otey, C.A., J. Cell Sci., 119,
995-1004 (2006).
4. Dixon, R.D.S. et al.
MOUSE ANTI-CYTOKERATIN 7
1990) 136:641-655.
Important Note: During shipment, small volumes of product will occasionally become entrapped in the seal of the product vial. For
products with volumes of 200 μL or less
Data Sheet - A3861
Biol., 150,
643-655 (2000).
2. Mykkanen, O.M. et al., Mol. Biol. Cell, 12, 3060-
3073 (2001).
3. Rachlin, A.S., and Otey, C.A., J. Cell Sci., 119,
995-1004 (2006).
4. Dixon, R.D.S. et al.
STED Sample Preparation Guide
A-21244 (rabbit)
ATTO 647N+ 647 775 Sigma-Aldrich 50185 (mouse)
40839 (rabbit)
ATTO 655 655 775 Sigma-Aldrich 50283 (mouse)
78519 (rabbit)w
Alexa Fluor 660 660 775 life technologies A
Data Sheet - T3405
a soluble end
product that is blue in color and can be read
spectrophotometrically at 370 or 655 nm. The reaction
may be stopped with 2 M H2SO4, resulting in a yellow
solution that is read at
Data Sheet - T5525
produces a soluble end product that is blue in
color and can be read spectrophotometrically at 370 or
655 nm. The reaction maybe stopped with 2 M H2SO4,
resulting in a yellow solution that is read at 450
MOUSE ANTI-CYTOKERATIN 7
Schaafsma, A.
Huijsmans, H. Robben, G. Schaart and P. Vooijs. American Journal of Pathology
136:641-655, 1990
2. Immunohistochemical demonstration of Keratin 7 in routinely fixed paraffin-embedded
Product_Information_Sheet-T3405
produces a soluble end product that is blue in color and can be read
spectrophotometrically at 370 or 655 nm. The reaction may be stopped with 2 M H2SO4, resulting in a
yellow solution that is read at 450
MOUSE ANTI-PROLIFERATING CELL NUCLEAR ANTIGEN (PCNA)
centrifuging at 200 x g for 10 minutes in each wash.
3 . Incubate the cells in 100 μlof a I:20 dilution of fluorescein-conjugated goat anti-mouse IgG.
4. Wash the cells twice with PBS, centrifuging at 200 x g for
Data Sheet - F7805 - Lot 061M0758
34
43
72
95
130
170
56
26
Figure 2.
Specific Activity of Lot Number 061M0758:
655 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5
Data Sheet - F7805 - Lot 041M0995
34
43
72
95
130
170
56
26
Figure 2.
Specific Activity of Lot Number 041M0995:
655 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5
Data Sheet - F7805 - Lot 039K0621
34
43
72
95
130
170
56
26
Figure 2.
Specific Activity of Lot Number 039K0621:
655 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5
Data Sheet - F7805 - Lot 019K1601
34
43
72
95
130
170
56
26
Figure 2.
Specific Activity of Lot Number 019K1601:
655 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5
Data Sheet - F7805 - Lot 029K0694
34
43
72
95
130
170
56
26
Figure 2.
Specific Activity of Lot Number 029K0694:
655 nmole/min/mg
Procedure
Preparation Instructions
Kinase Assay Buffer – 25 mM MOPS, pH 7.2, 12.5
Product Information Sheet-T5525
soluble end
product,2 which is blue in color and can be read
spectrophotometrically at 370 nm or 655 nm. The
reaction can be stopped with 2 M sulfuric acid (H2SO4)
and read at 450 nm.
Each
Product Information Sheet - M5557
10 minutes.
4. Wash the wells three times, 300 µl per well, with
PBS containing 0.05% TWEEN 20 to remove the
nonbiotinylated, complimentary strand of the PCR
product.
5. Add 200 µl per well of
Product Information Sheet - M3433
10 minutes.
4. Wash the wells three times, 300 µl per well, with
PBS containing 0.05% TWEEN 20 to remove the
nonbiotinylated, complimentary strand of the PCR
product.
5. Add 200 µl per well of
Product Information Sheet - M4058
10 minutes.
4. Wash the wells three times, 300 µl per well, with
PBS containing 0.05% TWEEN 20 to remove the
nonbiotinylated, complimentary strand of the PCR
product.
5. Add 200 µl per well of
Product Information Sheet - Z382469
for 5–10 minutes.
4. Wash the wells three times, 300 µl per well,
with PBS + 0.05% Tween-20 to remove the
nonbiotinylated, complimentary strand of the
PCR product.
5. Add 200 µl per well of a hapten
Product Information Sheet - MAK112
Cancer. Surg. Today,
33, 655–661 (2003).
Triton is a trademark of The Dow Chemical Company or
an affiliated company of Dow.
SS,KVG,LS,MAM 02/20-1
XP725 Antibody Number-Lot Number 071M4826
603 604 625 626 627 628 649 650 651 652
7.2 581 582 583 584 605 606 607 608 629 630 631 632 653 654 655 656
7.3 585 586 587 588 609 610 611 612 633 634 635 636 657 658
Bioassay Section of Plant Biotech Guide
viability
1 kit sufficient for 200 standard tests
Components:
Detailed technical bulletin, 1 each
5· extraction buffer, 25 mL
b-Glucuronidase, 1,000 units
4-MU, 25 mg
4-MU-GlcA, 25 mg
Stop solution, 100
Corning Deckworks pipette tips
10 960 4
4115 0.1-10 Natural No 10 960 4
4121 1-200 Natural Yes 10 960 4
4116 1-200 Natural No 10 960 4
4122 1-200 Yellow Yes 10 960 4
4117 1-200 Yellow No 10 960 4
4123 1-300 Natural Yes 10 960 4
4118 1
Product Information Sheet - 11383213001
001
11 759 051 001
CDP-Star, ready-to-use 2 x 50 ml 12 041 677 001
CSPD 1 ml
2 x 1 ml
4 x 1 ml
11 655 884 001
11 759 035 001
11 759 043 001
CSPD, ready-to-use 2
Immobilon Transfer Membranes: For superior protein and nucleic acid blots
x 5 min
4. Incubate with secondary 1 hr 30 min
antibody
5. Wash the membrane 3 x 10 min 3 x 5 min
6. Add substrate 10 min 10 min
Total time 4 hr 10 min
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