225-146-4

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4 Categories of Greener Alternatives

See how we classify the impact of our Greener Alternatives Platform.

4 类更环保的替代品

了解我们如何对绿色替代品平台的影响进行分类。

Papo com Ciência 4: o futuro sustentável dos negócios

Puriﬁcation or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens

This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.

Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow

This page shows how to separate IgG antibodies by afﬁnity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.

Colorimetric Detection of Western blot using 4-Chloro-1-Naphthol Solution Protocol

This solution is used as a substrate for detecting horseradish-peroxidase conjugates in Western blotting.

Thermal Transitions of Homopolymers: Glass Transition & Melting Point

Literature values for the glass transition temperature, (Tg), and melting temperature, (Tm), are given for the more common homopolymers.

Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels

Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels

Nickel Carbonyl

Analysis of nickel carbonyl, nickel carbonyl in air, organometallics in air, exposure limits for nickel carbonyl, ni(co)4

Silicon Nitride Atomic Layer Deposition: A Brief Review of Precursor Chemistry

atomic layer deposition (ALD), microelectronics, Mo:Al2O3 films, nanocomposite coating, photovoltaics, semiconductor devices, W:Al2O3 films, composite films, layer-by-layer

采用单克隆抗体亲和凝胶进行的FLAG融合蛋白免疫沉淀

采用M2单克隆抗体4％琼脂糖亲和凝胶进行的FLAG融合蛋白免疫沉淀（IP）实验方案

Derivatization Agents for LC/MS – An Improved Detection of Estradiol with ESI-MS

Improved LC/MS Detection of Estradiol using 4-(Dimethylamino) benzoyl chloride (DMABC) derivatization agent relative to dansyl chloride.

Software Simplifies Compliance with 21 CFR Part 11 and EudraLex Good Manufacturing Practice Volume 4 Annex 11

How software can simplify 21 CFR Part 11 and EudraLex Volume 4 Annex 11 compliance and provide additional benefits such as access control, audit trails and easier search and retrieval.

Chromolith® WidePore 300 HPLC Columns

Analytical Chromolith® HPLC columns for Bio applications, 300A mesopores: RP-18, RP-8, RP-4, Prot-A, Epoxy

Heavy Metal Analysis of Cannabis sativa by ICP-MS and the Need for Proper Sample Homogenization

A 4-step comprehensive ICP-MS workflow including sample homogenization, with standard addition calibration method, to determine heavy metals in Cannabis sativa.

完整全转录组扩增试剂盒实验方案(WTA2)

WTA2（一种全转录组扩增（WTA）方法）能够在区区4小时内，代表性地扩增出纳克级的总RNA而没有3'偏差。

MSI检测和IHC

结直肠癌是大多数西方国家居民中普遍发生的疾病，仅次于肺癌。

Sample Preparation for Chromatographic Purification

This page discusses various aspects of sample preparation for chromatographic purification.

ELISA Protocols

This article offers 4 popular ELISA protocols: Sandwich ELISA protocol, Phosphorylation Assay Procedure, EIA Assay Procedure, & Cell-based Assay Procedure.

Complete Whole Transcriptome Amplification Kit Protocol (WTA2)

WTA2, a Whole Transcriptome Amplification (WTA) method, allows for representative amplification of nanogram quantities of total RNA in less than 4 hours without 3-bias

Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B

This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.

In Vitro Differentiation of Human iPS Cells into Airway Epithelial Lung Organoids for Respiratory Disease Research Applications

Lung organoids are valuable 3D models for human lung development and respiratory diseases. The 3dGRO™ differentiation protocol generates organoids from human iPSCs in 4 steps.

ELISA实验方法

该文章提供了4种常用的ELISA实验方法：夹心法ELISA实验方法、磷酸化检测过程、EIA检测过程以及基于细胞的检测过程

The Formulation Box - Biomolecules Stabilization and Aggregation Prevention

You are cordially invited to discover in 4 steps how you can improve your strategies for biomolecules formulation development and extend your knowledge about viscosity reduction capabilities

The Formulation Box - Biomolecules Stabilization and Aggregation Prevention

You are cordially invited to discover in 4 steps how you can improve your strategies for biomolecules formulation development and extend your knowledge about viscosity reduction capabilities

Duolink® PLA多色检测方案

使用荧光显微镜和多色试剂同时检测、可视化和定量一个组织或细胞样本中多达4种的蛋白、蛋白修饰或蛋白互作。

Coupling Protocol for Primary Amine of a Ligand

This page shows coupling through the primary amine of a ligand with a NHS-activated Sepharose High Performance, NHS-activated Sepharose 4 Fast Flow and CNBr-activated Sepharose from Cytiva.

Enzymatic Assay of Cathepsin B

Cathepsin B is a lysosomal cysteine proteinase with broad specificity. This protocol uses Nα–CBZ–Arg–Arg–7–amido–4–methylcoumarin as the substrate for fluorometric detection of Cathepsin B activity.

Removal of Detergents from Biological Product Matrices

The Amberlite™ XAD-4 resin used in Porozorb™ cartridges is a proven technology that is highly effective in removing various detergents from cell culture media for biopharmaceutical applications such as vaccine production.

Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens

This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.

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