73-80
(1996).
7. Aigner, T. et al., Histochem. Cell Biol., 107, 435-40
(1997).
8. Girkontaite, I. et al., Matrix Biol., 15, 231-8 (1996).
9. Yamaguchi, N. et al., J. Biol. Chem., 264, 16022-
monomers, polymers, and additives from Aldrich, please
contact our Technical Services Department at (800) 231-8327 (USA) or e-mail us at aldrich@sial.com.
Plasticizers
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J. Neuroscience Methods 107: 131-140.
Draberova, E. et al. (1998). Expression of class III beta-tubulin in normal and neoplastic human
tissues. Histochem. Cell Biol. 109: 231-239.
Important
J. Neuroscience Methods 107: 131-140.
Draberova, E. et al. (1998). Expression of class III beta-tubulin in normal and neoplastic human
tissues. Histochem. Cell Biol. 109: 231-239.
Important
, pH 7.4.
9. 2% paraformaldehyde in PBS.
10. Whole blood lysing solution.
11. Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
PBS, pH 7.4.
9. 2% paraformaldehyde in PBS.
10. Whole blood lysing solution.
11. Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
diluent
saline, pH 7.4.
9. 2% paraformaldehyde in PBS.
10. Whole blood lysing solution.
11. Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in dilu
PBS, pH 7.4.
9. 2% paraformaldehyde in PBS.
10. Whole blood lysing solution.
11. Flow cytometer.
Procedure
1. a. Use 100 L of whole blood OR
b. Adjust cell suspension to 1 x 107 cells/mL in
diluent
, pH 7.4.
10. 2% paraformaldehyde in PBS.
11. Whole blood lysing solution.
12. Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
diluent
1. Pellet 2 × 107 cells by centrifugation at
600 g for 10 minutes.
2. Carefully remove and discard the
supernatant.
Tissues
1. Isolate the tissue of interest (~100 mg).
2. Immerse the
PBS, pH 7.4.
9. 2% paraformaldehyde in PBS.
10. Whole blood lysing solution.
11. Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
diluent
Store all reagents at 2 – 8°C
Reagents Supplied
Catalog
Number
Volume Quantity
HepG2 Cell Lysate Control 47-231 lyophilized 1 vial
Set of one 96-Well Plate with 2
sealers
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