remove the salt solution by aspiration and
discard.
2. Add NO-ZYME™ (approximately 2 mL/25 cm2 or
5 mL/75 cm2 flask) and rock the vessel to coat the cells
completely. After 1 - 2 minutes at room temperature
prostate.
4,5
In 10% of breast
cancer patients this gene is amplified, and in 44% the
protein is overexpressed.
5
Monoclonal antibodies to KNCK9 are an important tool
for studying the potassium
with 8 mM L-glutamine at a density of
5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Incubate the cells at 37 C in a humidified incubator with
8 - 10% CO2.
5.
with 8 mM L-glutamine at a density of
5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Incubate the cells at 37 C in a humidified incubator with
8 - 10% CO2.
5. Continue
Development. 2003 Feb;130(4):775-84.
3. Development. 2000 Mar;127(6):1231-41.
4. Neuron. 1994 May;12(5):957-75.
5. Adv Exp Med Biol. 2002;515:91-102.
Important Note: During shipment, small volumes of
binding to the resin.
1 3 5 7
Reproducibility
Lane 1: Molecular
Weight Markers
Lanes 2–11: Replicates
Figure 2. Ten replicates of a human plasma sample (75 µL) were diluted with 125 µL
is
as follows:
Amino Acid Amount (mg/g)
Ala 30
Arg 31
Asp 67
Cys 3
Glu 186
Gly 19
His 22
Ile 44
Leu 75
Lys 68
Met 27
Phe 40
Pro 88
Ser 51
Thr 42
Trp 10
Tyr 28
Val 59
The total amino acid content is
with 8 mM L-glutamine at a density of
5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Incubate the cells at 37 C in a humidified incubator with
8 - 10% CO2.
5. Continue
with 8 mM L-glutamine at a density of
5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks.
4. Incubate the cells at 37 C in a humidified incubator with
8 - 10% CO2.
5. Continue
59321C) and trypsinize with 5 mL of trypsin.
Add 15 mL of EX-CELLTM 302 + 2% FBS. Obtain a cell
count and pellet the cells by centrifugation.
3. Passage cells into a 75 cm2 flask containing 30 mL
EX-CELLTM
59321C) and trypsinize with 5 mL of trypsin.
Add 15 mL of EX-CELLTM 302 + 2% FBS. Obtain a cell
count and pellet the cells by centrifugation.
3. Passage cells into a 75 cm2 flask containing 30 mL
EX-CELLTM