显示 1-17 共 17 条结果 关于 "C270"
Honglin Chen et al.
The Journal of biological chemistry, 285(4), 2368-2374 (2009-12-01)
Androgen ablation therapy is effective in treating androgen-dependent prostate tumors; however, tumors that can proliferate in castrate levels of androgen eventually arise. We previously reported that in CWR22Rv1 (Rv1) cells, the protease calpain 2 can cleave the androgen receptor (AR)...
Manting Du et al.
Food chemistry, 274, 743-749 (2018-10-31)
The objective of this study was to investigate the effect of phosphorylation on the sensitivity of μ-calpain to the inhibition induced by calpastatin. Purified μ-calpain was incubated with alkaline phosphatase (AP) or protein kinase A (PKA) to modulate the phosphorylation...
Taly Vaisid et al.
Biochimica et biophysica acta, 1833(10), 2369-2377 (2013-06-19)
Calpain (intracellular Ca(2+)-dependent protease) and calpastatin (calpain specific endogenous inhibitor) are widely distributed in biological systems, and have been implicated in many cellular physiological and pathological processes. Calpastatin level is of central importance to the control of calpain activity. We...
H Persson et al.
Brain research, 611(2), 272-278 (1993-05-21)
The localization of the two Ca-activated extralysosomal proteases m-calpain and mu-calpain in the eye of the adult rabbit was examined by immunohistochemistry, using poly- and monoclonal antibodies against the corresponding rabbit antigens. Immunoreactivity against the two forms of calpains was...
Interaction between calpain-1 and HSP90: new insights into the regulation of localization and activity of the protease.
Averna, et al.
PLoS ONE, 10, e0116738-e0116738 (2019)
Lan Zhang et al.
PloS one, 8(8), e70935-e70935 (2013-08-21)
Recent studies point to an association between the late-onset sporadic Parkinson's disease (PD) and single nucleotide polymorphisms (SNPs) rs1559085 and rs27852 in Ca(2+)-dependent protease calpain inhibitor calpastatin (CAST) gene. This finding is of interest since loss of CAST activity could...
Xiaohui Li et al.
Cornea, 32(5), 696-701 (2013-03-02)
Keratoconus (KC) is a genetically heterogeneous corneal dystrophy. Previously, we performed 2 genome-wide linkage scans in a 4-generation autosomal dominant pedigree and repeatedly mapped a KC locus to a genomic region located on chromosome 5q overlapping the gene encoding the...
Roberta Benetti et al.
The Journal of biological chemistry, 280(23), 22070-22080 (2005-04-09)
Beta-catenin is a multifunctional protein serving both as a structural element in cell adhesion and as a signaling component in the Wnt pathway, regulating embryogenesis and tumorigenesis. The signaling fraction of beta-catenin is tightly controlled by the adenomatous polyposis coli-axin-glycogen...
R D Lane et al.
Experimental cell research, 203(1), 5-16 (1992-11-01)
Little is known about the relative intracellular localizations of the calcium-dependent proteases, calpains, and their naturally occurring inhibitor, calpastatin. In the present study, the intracellular localization of mu-calpain, m-calpain, and calpastatin was studied at the light microscopic level in proliferating...
M Averna et al.
Cellular and molecular life sciences : CMLS, 60(12), 2669-2678 (2003-12-20)
The amount of calpastatin directly available in cytosol is under the control of [Ca2+] and [cyclic AMP]. Prolonged calpain activation also promotes degradation of calpastatin. The fluctuation of calpastatin concentration in cell soluble fraction is accompanied by an initial decrease...
H Ma et al.
Journal of biochemistry, 113(5), 591-599 (1993-05-01)
Calpain requires Ca2+ for both proteolysis of its substrates and interaction with its endogenous inhibitor, calpastatin. The mechanism of inhibition of calpain by calpastatin has remained unsolved, although Nishimura and Goll [J. Biol. Chem. 266, 11842-11850 (1991)] reported that autolyzed...
Peggy Raynaud et al.
Archives of biochemistry and biophysics, 437(1), 69-77 (2005-04-12)
Calpastatin is a specific endogenous protein inhibitor of the ubiquitous calcium dependent proteinases mu- and m-calpain. The calpain-calpastatin system is involved in various physiological and pathological processes. In the present study, we determined the bovine calpastatin gene structure and demonstrated...
Thangavel Mahalingam Vijayakumar et al.
Journal of food science and technology, 52(7), 4537-4543 (2015-07-04)
Cytochrome P450 (CYP450) inhibition by the bioactive molecules of dietary supplements or herbal products leading to greater potential for toxicity of co-administered drugs. The present study was aimed to compare the inhibitory potential of selected common dietary bioactive molecules (Gallic...
Steven J Ennion et al.
Molecular pharmacology, 61(2), 303-311 (2002-01-26)
P2X receptors contain 10 conserved cysteines in the extracellular loop. To investigate whether these residues form disulfide bonds, we created a series of single and double cysteine-alanine mutants in the human P2X(1) receptor. Mutants were expressed in Xenopus laevis oocytes...
M B Rokic et al.
Physiological research, 59(6), 927-935 (2010-04-22)
Mammalian P2X receptors contain 10 conserved cysteine residues in their ectodomains, which form five disulfide bonds (SS1-5). Here, we analyzed the relevance of these disulfide pairs in rat P2X4 receptor function by replacing one or both cysteines with alanine or...
H N Yeowell et al.
Matrix biology : journal of the International Society for Matrix Biology, 19(1), 37-46 (2000-02-25)
This study describes the relative contribution of the 10 cysteine residues in lysyl hydroxylase 1 (LH1) to enzyme activity. We have identified a novel mutation of a 15-bp deletion in exon 11 in one LH1 allele, that codes for amino...
Antara Banerjee et al.
Biochemistry, 44(24), 8908-8917 (2005-06-15)
The residues involved in substrate interaction of the human apical sodium-dependent bile acid transporter (hASBT, SLC10A2) remain undefined. Biochemical modification of conserved cysteine residues has suggested their direct involvement in hASBT function. In the present study, we developed novel methanethiosulfonyl...

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