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Sahul H Rajamohamed et al.
Plant foods for human nutrition (Dordrecht, Netherlands), 68(3), 306-312 (2013-08-06)
Glabrous or hairless canaryseed is a nutritional grain that could be a good addition to the diet if approved as a novel food. To assess the impact of thermal treatment on its digestibility; raw, roasted or boiled flours prepared from...
ALPHA-CHYMOTRYPSIN AND THE NATURE OF ENZYME CATALYSIS.
C NIEMANN
Science (New York, N.Y.), 143(3612), 1287-1296 (1963-03-20)
Jesús J Rochín-Medina et al.
International journal of food sciences and nutrition, 66(5), 498-504 (2015-07-15)
The aim of this work was to obtain a functional flour with enhanced nutritional properties, and antioxidant and antihypertensive potential from black bean seeds by optimizing the solid state bioconversion (SSB) process using a Rhizopus oligoporus strain. Response surface methodology...
Patrícia Anacleto et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 145, 111717-111717 (2020-09-06)
Human exposure to mercury (Hg) and methylmercury (MeHg) through the ingestion of seafood raises human health-related concerns. In contrast, green tea has health benefits and its consumption potentially reduces bioaccessibility of dietary Hg. The present study aimed to assess the...
Maria Galini Faidra Angelerou et al.
Journal of controlled release : official journal of the Controlled Release Society, 317, 118-129 (2019-11-05)
Supramolecular gels have recently emerged as promising biomaterials for the delivery of a wide range of bioactive molecules, from small hydrophobic drugs to large biomolecules such as proteins. Although it has been demonstrated that each encapsulated molecule has a different...
Y Birk
International journal of peptide and protein research, 25(2), 113-131 (1985-02-01)
Four decades of studies on the isolation, characterization, properties, structure, function and possible uses of the Bowman-Birk trypsin- and chymotrypsin-inhibitor from soybeans are reviewed. Starting from Bowman's Acetone Insoluble factor, designated Ai, AA and SBTIAA, the Bowman-Birk inhibitor (BBI) was...
W Russ Algar et al.
ACS nano, 6(12), 11044-11058 (2012-12-12)
Semiconductor nanocrystals, or quantum dots (QDs), are one of the most widely utilized nanomaterials for biological applications. Their cumulative physicochemical and optical properties are both unique among nanomaterials and highly advantageous. In particular, Förster resonance energy transfer (FRET) has been...
Chad Cummings et al.
Biomaterials, 34(30), 7437-7443 (2013-07-16)
Polymer-based protein engineering (PBPE) offers an attractive method to predictably modify and enhance enzyme structure and function. Using polymers that respond to stimuli such as temperature and pH, enzyme activity and stability can be predictably modified without a dependence on...
Vanessa Tubero Euzebio Alves et al.
Infection and immunity, 81(12), 4399-4407 (2013-09-18)
Protease-activated receptor 2 (PAR2) is implicated in the pathogenesis of chronic inflammatory diseases, including periodontitis; it can be activated by gingipain and produced by Porphyromonas gingivalis and by neutrophil protease 3 (P3). PAR2 activation plays a relevant role in inflammatory...
M A Abd El-Ghaffar et al.
Carbohydrate polymers, 92(2), 2095-2102 (2013-02-13)
Chitosan grafted with polymethyl methacrylate (PMMA-g-CS) was prepared via a free-radicals polymerization technique as a carrier for enzyme immobilization. α-Chymotrypsin (CT), as an enzyme model in this study, was immobilized onto the prepared PMMA-g-CS via covalent bonding. Calcium alginate (CA)...
PRSS1 c.623G>C (p.G208A) variant is associated with pancreatitis in Japan.
Atsushi Masamune et al.
Gut, 63(2), 366-366 (2013-05-21)
Jaroslaw Czubinski et al.
Food chemistry, 143, 418-426 (2013-09-24)
This study describes in vitro digestion of lupin seed globulins by pancreatin, trypsin and chymotrypsin. Lupin seed globulins turned out to be almost totally susceptible to chymotrypsin digestion. When panceratin or trypsin were used for digestion of lupin seed globulins...
Jack B Maguire et al.
Proteins, 89(4), 436-449 (2020-11-30)
The FastDesign protocol in the molecular modeling program Rosetta iterates between sequence optimization and structure refinement to stabilize de novo designed protein structures and complexes. FastDesign has been used previously to design novel protein folds and assemblies with important applications...
Randy Adjonu et al.
Food chemistry, 136(3-4), 1435-1443 (2012-12-01)
Heat pre-treated and non heat pre-treated whey protein isolate (WPI) were hydrolysed using α-chymotrypsin (chymotrypsin), pepsin and trypsin. The in vitro antioxidant activity, ACE-inhibition activity and surface hydrophobicities of the hydrolysates were measured in order to determine if peptides with...
Rossana D'Arienzo et al.
Cytokine, 48(3), 254-259 (2009-09-09)
Probiotic strains play an important role in modulating activities in the gut-associated lymphoid tissue. Elucidation of the mechanisms that mediate probiotic-driven immunomodulation may facilitate their therapeutic application for specific immune-mediated diseases or for prophylaxis. In this study, we explored the...
Susan Matthew et al.
Planta medica, 75(5), 528-533 (2009-02-14)
Three unusual tiglic acid-containing cyclodepsipeptides, possessing the revised regioisomeric structures for largamides A-C (1-3), have been isolated from the marine cyanobacterium Lyngbya confervoides collected from southeastern Florida. The two-dimensional structures were determined by NMR spectroscopy and the absolute configurations by...
Sara P Cuellar-Bermúdez et al.
Journal of the science of food and agriculture, 97(15), 5123-5130 (2017-04-22)
Given the importance of A. platensis as a potential food protein source, we describe an affordable deodorization process that does not significantly affect the nutritional value of algae biomass. Ethanol, acetone or hexane were used to deodorize algae biomass and then...
[Stages of hydrolysis catalyzed by trypsin and chymotrypsin].
V M Krutiakov
Izvestiia Akademii nauk SSSR. Seriia biologicheskaia, 5, 671-680 (1973-09-01)
Gabriela Arévalo-Pinzón et al.
Scientific reports, 7(1), 9616-9616 (2017-09-01)
The malarial parasite's invasion is complex, active and coordinated, involving many low and high affinity interactions with receptors on target cell membrane. Proteomics analysis has described around 40 proteins in P. vivax which could be involved in reticulocyte invasion; few...
Gang Huang et al.
Nature communications, 8(1), 935-935 (2017-10-19)
Biological nanopores are nanoscale sensors employed for high-throughput, low-cost, and long read-length DNA sequencing applications. The analysis and sequencing of proteins, however, is complicated by their folded structure and non-uniform charge. Here we show that an electro-osmotic flow through Fragaceatoxin...
Llinos G Harris et al.
Applied and environmental microbiology, 79(4), 1393-1395 (2012-12-12)
Staphylococcus aureus and Staphylococcus epidermidis biofilms cause chronic infections due to their ability to form biofilms. The excretions/secretions of Lucilia sericata larvae (maggots) have effective activity for debridement and disruption of bacterial biofilms. In this paper, we demonstrate how chymotrypsin...
Antonio Jesús Vizcaíno et al.
Marine drugs, 18(6) (2020-06-24)
This piece of research evaluates the presence of protease inhibitors in the macroalga Ulva ohnoi and provides an initial overview of their mode of action. The ability of Ulva protease inhibitors to inhibit digestive proteases of three marine fish species...
Evolutionary divergence of substrate specificity within the chymotrypsin-like serine protease fold.
J J Perona et al.
The Journal of biological chemistry, 272(48), 29987-29990 (1997-12-31)
Jair Tonon et al.
BMC musculoskeletal disorders, 14, 39-39 (2013-01-25)
Peripheral skeletal muscle is altered in patients suffering from emphysema and chronic obstructive pulmonary disease (COPD). Oxidative stress have been demonstrated to participate on skeletal muscle loss of several states, including disuse atrophy, mechanical ventilation, and chronic diseases. No evidences...
Daixi Liu et al.
Gene, 514(2), 99-104 (2012-12-04)
A 255-bp cDNA encoding an 84-amino acid residue (aa) precursor protein containing 8 half-cysteines was cloned from the skin of the frog, Ceratophrys calcarata. By sequence comparison and signal peptide prediction, the precursor was predicted to release a 63-aa mature...
THE CHEMICAL MODIFICATION OF CHYMOTRYPSIN.
G H DIXON et al.
Canadian journal of biochemistry, 42, 695-714 (1964-05-01)
[The use of proteolytic enzymes (trypsin, chymotrypsin, streptokinase, streptodornase, papain) in stomatology].
A I Protasevich
Stomatologiia, 46(5), 107-109 (1967-09-01)
K Y Jiang et al.
Biochimica et biophysica acta, 1433(1-2), 198-209 (1999-08-14)
Chemical glycosylation of bovine alpha-chymotrypsin, by a glucosamine adduct on the carboxyl group, results in the modification of its catalytic activity. The structural alterations of alpha-chymotrypsin resulting from its glycosylation are studied by immobilized metal-ion affinity chromatography (IMAC) and immobilized...
A Ghaffar et al.
Journal of chromatography. A, 1286, 29-40 (2013-03-21)
The suitability of biomaterials for specific biomedical applications can be investigated through their in vitro biodegradability with selected enzymes and through their degradation kinetics. A system was developed for studying the enzymatic degradation of poly(ester amide)s (PEAs) coatings under sink...
Ciarán J Brennan et al.
Frontiers in plant science, 11, 433-433 (2020-06-02)
Understanding the nuances of host/pathogen interactions are paramount if we wish to effectively control cereal diseases. In the case of the wheat/Zymoseptoria tritici interaction that leads to Septoria tritici blotch (STB) disease, a 10,000-year-old conflict has led to considerable armaments...
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