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M L Gavala et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 43(2), 187-197 (2013-01-22)
Allergic airway inflammation contributes to the airway remodelling that has been linked to increased obstruction and morbidity in asthma. However, the mechanisms by which allergens contribute to airway remodelling in humans are not fully established. CCL18, chitotriosidase (CHIT1) and YKL-40
Qinghua Wang et al.
Archives of virology, 158(12), 2523-2530 (2013-07-06)
Dendrolimus kikuchii Matsumura nucleopolyhedrovirus (DkNPV) is a novel nucleopolyhedrovirus strain that has exhibited high potential as biological control agent against D. kikuchii. In this work, a 1755-bp DkChi gene with sequence homology to a chitinase gene was cloned from the
Frank W Smith et al.
EvoDevo, 9, 19-19 (2018-08-03)
Both euarthropods and vertebrates have tripartite brains. Several orthologous genes are expressed in similar regionalized patterns during brain development in both vertebrates and euarthropods. These similarities have been used to support direct homology of the tripartite brains of vertebrates and
Jolanda M van Munster et al.
Microbiology (Reading, England), 159(Pt 9), 1853-1867 (2013-07-09)
Sporulation is an essential part of the life cycle of the industrially important filamentous fungus Aspergillus niger. The formation of conidiophores, spore-bearing structures, requires remodelling of the fungal cell wall, as demonstrated by the differences in carbohydrate composition of cell
Sara Strobel et al.
PloS one, 8(9), e72770-e72770 (2013-09-11)
The gastrointestinal tract of animals is adapted to their primary source of food to optimize resource use and energy intake. Temperate bat species mainly feed on arthropods. These contain the energy-rich carbohydrate chitin, which is indigestible for the endogenous enzymes
Tomoyasu Hirose et al.
Proceedings of the National Academy of Sciences of the United States of America, 110(40), 15892-15897 (2013-09-18)
The Huisgen cycloaddition of azides and alkynes, accelerated by target biomolecules, termed "in situ click chemistry," has been successfully exploited to discover highly potent enzyme inhibitors. We have previously reported a specific Serratia marcescens chitinase B (SmChiB)-templated syn-triazole inhibitor generated
Sujata S Chaudhari et al.
PLoS genetics, 9(1), e1003268-e1003268 (2013-02-06)
Molting, or the replacement of the old exoskeleton with a new cuticle, is a complex developmental process that all insects must undergo to allow unhindered growth and development. Prior to each molt, the developing new cuticle must resist the actions
Paula Gutiérrez-Pérez et al.
Science advances, 7(42), eabh1434-eabh1434 (2021-10-16)
[Figure: see text].
Xiaoqing Liu et al.
Journal of agricultural and food chemistry, 60(51), 12477-12481 (2012-12-12)
Edible bird's nest (EBN) is made of the swiftlets' saliva, which has attracted rather more attention owing to its nutritious and medical properties. Although protein constitutes the main composition and plays an important role in EBN, few studies have focused
Shigeo Suzuki et al.
International journal of biological macromolecules, 43(1), 13-19 (2007-11-06)
Allosamidin is a family 18 chitinase inhibitor produced by Streptomyces. In its producing strain, Streptomyces sp. AJ9463, allosamidin promotes production of the family 18 chitinase originated from chi65 in a chitin medium through the two-component regulatory system encoded by chi65R
Yi Zhang et al.
Frontiers in plant science, 8, 1880-1880 (2017-11-23)
During pathogenic infection, hundreds of proteins that play vital roles in the
Chitinase in some Basidiomycetes.
The Biochemical journal, 61(4), 579-586 (1955-12-01)
J Philip Nance et al.
PLoS pathogens, 8(11), e1002990-e1002990 (2012-12-05)
Chronic infections represent a continuous battle between the host's immune system and pathogen replication. Many protozoan parasites have evolved a cyst lifecycle stage that provides it with increased protection from environmental degradation as well as endogenous host mechanisms of attack.
A A Monoszon et al.
Bulletin of experimental biology and medicine, 154(1), 40-43 (2013-01-19)
Mouse chitotriosidase cleaving chitin belongs to the family of mammalian chitinases, whose biological functions are poorly understood. Chitotriosidase activity in mouse serum was shown to be much higher than in humans. The following interstrain differences were revealed in mouse chitotriosidase
Anna M Kielak et al.
Applied and environmental microbiology, 79(1), 263-272 (2012-10-30)
Chitin amendment is a promising soil management strategy that may enhance the suppressiveness of soil toward plant pathogens. However, we understand very little of the effects of added chitin, including the putative successions that take place in the degradative process.
Xinyu Liu et al.
Bio-protocol, 7(5), e2164-e2164 (2017-03-05)
Chitin is a linear polysaccharide composed of β (1→4)-linked N-acetylglucosamine (GlcNAc) residues. In fungi, chitin is an important component of the cell wall. Here, we provide a protocol to measure the chitin content of fungal cells using Magnaporthe oryzae as
Christine Klinger et al.
Marine drugs, 17(2) (2019-03-01)
Sponges are a valuable source of natural compounds and biomaterials for many biotechnological applications. Marine sponges belonging to the order Verongiida are known to contain both chitin and biologically active bromotyrosines. Aplysina archeri (Aplysineidae: Verongiida) is well known to contain
Clemens J Heilmann et al.
Eukaryotic cell, 12(2), 254-264 (2012-12-18)
The human fungal pathogen Candida albicans can grow at temperatures of up to 45°C. Here, we show that at 42°C substantially less biomass was formed than at 37°C. The cells also became more sensitive to wall-perturbing compounds, and the wall
Camilla Kappe et al.
Journal of neuroinflammation, 9, 276-276 (2012-12-25)
Type 2 diabetes (T2D) is a strong risk factor for developing neurodegenerative pathologies. T2D patients have a deficiency in the intestinal incretin hormone GLP-1, which has been shown to exert neuroprotective and anti-inflammatory properties in the brain. Here we investigate
Jarrod R Fortwendel et al.
Antimicrobial agents and chemotherapy, 54(4), 1555-1563 (2010-02-04)
Attenuated activity of echinocandin antifungals at high concentrations, known as the "paradoxical effect," is a well-established phenomenon in Candida albicans and Aspergillus fumigatus. In the yeast C. albicans, upregulation of chitin biosynthesis via the protein kinase C (PKC), high-osmolarity glycerol
Wen-Jia Yang et al.
International journal of molecular sciences, 14(10), 20048-20063 (2013-10-12)
Insect chitinases are hydrolytic enzymes that are required for the degradation of glycosidic bonds of chitin. In this study, we identified and characterized a full-length cDNA of the chitinase gene (BdCht2) in the oriental fruit fly, Bactrocera dorsalis. The cDNA
Krisztian Twaruschek et al.
Frontiers in microbiology, 9, 1954-1954 (2018-09-28)
In filamentous fungi such as Fusarium graminearum, disruption of multiple genes of interest in the same strain (e.g., to test for redundant gene function) is a difficult task due to the limited availability of reliable selection markers. We have created
Marcio L Rodrigues et al.
Eukaryotic cell, 7(4), 602-609 (2007-11-28)
The capsule of Cryptococcus neoformans is a complex structure whose assembly requires intermolecular interactions to connect its components into an organized structure. In this study, we demonstrated that the wheat germ agglutinin (WGA), which binds to sialic acids and beta-1,4-N-acetylglucosamine
Laura Bertini et al.
Biological chemistry, 393(12), 1533-1546 (2013-03-06)
Plants possess an innate immune system enabling them to defend themselves against pathogen attack.The accumulation of newly synthesized pathogenesis related proteins (PRs) is one of the most studied inducible plant defence response. In this paper, we report on the characterization
Melissa S Hillwig et al.
Journal of plant physiology, 168(7), 734-738 (2010-11-26)
Previous SDS PAGE gel analysis of the floral nectars from petunia and tobacco plants revealed significant differences in the protein patterns. Petunia floral nectar was shown to contain a number of RNase activities by in gel RNase activity assay. To
Yongjun Tang et al.
Journal of nanoscience and nanotechnology, 12(10), 8081-8086 (2013-02-21)
The chitinase producing bacterium H3 strain was screened from nature with the selective medium and was identified as Bacillus thuringiensis. The purpose of this investigation is to study the antifungi activity of Bacillus thuringiensis H3 chitinase and its immobilized particles
Vasiliki Skiada et al.
The New phytologist, 226(5), 1429-1444 (2020-01-31)
Legumes interact with a wide range of microbes in their root systems, ranging from beneficial symbionts to pathogens. Symbiotic rhizobia and arbuscular mycorrhizal glomeromycetes trigger a so-called common symbiotic signalling pathway (CSSP), including the induction of nuclear calcium spiking in
Frank Hoster et al.
Applied microbiology and biotechnology, 66(4), 434-442 (2004-08-04)
Thirteen different chitin-degrading bacteria were isolated from soil and sediment samples. Five of these strains (SGE2, SGE4, SSL3, MG1, and MG3) exhibited antifungal activity against phytopathogenic fungi. Analyses of the 16S rRNA genes and the substrate spectra revealed that the
Krzysztof Nowacki et al.
Marine drugs, 18(6) (2020-06-06)
The development of novel and effective methods for the isolation of chitin, which remains one of the fundamental aminopolysaccharides within skeletal structures of diverse marine invertebrates, is still relevant. In contrast to numerous studies on chitin extraction from crustaceans, mollusks
Fei Liu et al.
Fungal genetics and biology : FG & B, 136, 103320-103320 (2019-12-22)
Leptosphaeria maculans is the causal agent of blackleg disease on Brassica napus. Determining the underlying functions of genes required for pathogenesis is essential for understanding the infection process. A chitin-binding protein (LmCBP1) was discovered as a pathogenicity factor for the
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