Cloning and Expression

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Polyamino acids adopt ordered conformations with unique biological properties and functions like α-helices and β-sheets.

Competent Cell Selection & E. coli Markers Guide

Select competent cells by application, transformation efficiency, and genotype. We also present a list of E. coli markers for reference.

Simplicon™ in vivo Protein Expression System

Simplicon: A non-genome integrating, tunable and sustained protein expression system in human cells, based on a single, polycistronic, and self-replicating synthetic RNA.

Troubleshooting in pGEX Expression Vectors

This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.

Introduction to Cell Transfection

Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.

Reverse Transfection of Plasmid DNA

Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.

3xFLAG® System Expression Vectors for Ultra-Sens

The 3xFLAG system enhances fusion protein detection, offering improved sensitivity over traditional methods.

Blue-White Screening & Protocols for Colony Selection

Learn about and find protocols for the blue white screen technique used in molecular biology research to identify recombinant bacterial clones for further analysis.

Bacterial Transformation Protocols

General protocols for growth of competent cells and their transformation (uptake of DNA).

Genotypes, Phenotypes and Markers

A genotype is a list of mutant genes in an organism. In addition to mutations of the genome, other genetic elements such as prophage or plasmids can also be included.

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