Cloning and Expression
应用筛选条件
应用:Cloning and Expression
内容物类型:Technical Article
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分子克隆的问题解决
分子克隆是将目的基因 (GOI) 插入到质粒载体中的过程，然后将该载体插入到表达 GOI 编码的蛋白质的细胞中。一旦蛋白质在细胞中得到表达，这种表达即可用于各种不同的研究（如细胞信号传导、形态学或其他方面）。
将目标基因克隆到质粒载体中
本克隆方案包括克隆系统和质粒载体选择、质粒限制性消化、片段限制性消化、凝胶切除、DNA去磷酸化等内容。
细菌转化实验方案
感受态细胞生长及其转化（吸收DNA）的通用实验方案。
蓝白斑筛选和菌落筛选实验方案
了解并寻找分子生物学研究所用的蓝白斑筛选技术实验方案，以鉴定重组细菌克隆并进行进一步分析。
感受态细胞选择及大肠杆菌标志物指南
按应用、转化效率和基因型选择感受态细胞。我们还提供了一系列的大肠杆菌标志物供参考。
T7启动子系统
细菌表达载体：T7启动子系统。可最大限度提高细菌表达水平的T7载体。
用于超灵敏检测重组蛋白的3xFLAG®系统表达载体
3xFLAG系统是原始系统的改进版本，融合了总共22个氨基酸的3个串联FLAG®表位。含有3xFLAG的融合蛋白的检测比任何其他系统提高多达200倍。
FLAG®标记细菌和哺乳动物表达载体
FLAG®和3xFLAG®表达载体和产品用于细菌和哺乳动物表达载体以及蛋白质检测和纯化。
用工程质粒转化大肠杆菌
制备感受态细胞；制备琼脂板；细菌转化；挑取菌落；在液体培养基种生长细菌；冻存细菌。
限制性内切核酸酶 — 分子剪
术语“限制酶”源自Werner Arber和Matthew Meselson实验室对肠杆菌噬菌体λ（λ噬菌体）的研究。
酵母转化简介
转化是将外源DNA引入细胞，引起遗传变异或基因修饰的过程。1928年，Griffith首次报道了利用肺炎链球菌进行的转化。Avery等人于1944年证明了DNA转化的原理。
细胞转染简介
转染是将DNA、RNA或蛋白质引入真核细胞的过程，用于研究和调节基因表达。因此，转染技术和实验方案作为分析工具，有助于表征遗传功能、蛋白质合成、细胞生长和发育。
Bacterial Transformation Protocols
General protocols for growth of competent cells and their transformation (uptake of DNA).
Nucleic Acid Modifying Enzyme Selection Chart (Ultra Pure)
A helpful chart for selection of correct nucleic acid modifying enzyme.
Transforming E.coli with Engineered Plasmid
Making Competent Cells; Making Agar Plates; Bacterial Transformation; Picking Colonies; Growing Bacteria in Liquid Culture; Freezing Bacteria.
T7 Promoter System
Bacterial Expression Vectors: T7 Promoter System. T7 Vectors for Highest Expression Levels in Bacteria.
Restriction Endonucleases - The Molecular Scissors
The term “Restriction enzyme” originated from the studies of Enterobacteria phage λ (lambda phage) in the laboratories of Werner Arber and Matthew Meselson.
Genotypes, Phenotypes and Markers
A genotype is a list of mutant genes in an organism. In addition to mutations of the genome, other genetic elements such as prophage or plasmids can also be included.
Synthesis and Biomedical Applications of Polyamino Acids
Polyamino acids are able to adopt ordered conformations, such as α-helices and β-sheets, through cooperative hydrogen bonding. These conformations impart polyamino acids with various unique properties and functions in biological environments.
Introduction to Cell Transfection
Transfection is the introduction of DNA, RNA, or proteins into eukaryotic cells and is used in research to study and modulate gene expression. Thus, transfection techniques and protocols serve as an analytical tool that facilitates the characterization of genetic functions
Competent Cell Selection & E. coli Markers Guide
Select competent cells by application, transformation efficiency, and genotype. We also present a list of E. coli markers for reference.
Troubleshooting in pGEX Expression Vectors
This page describes troubleshooting strategies for cloning the gene or gene fragment into a pGEX expression vector.
Cloning Genes-of-Interest into a Plasmid Vector
This cloning protocol includes selecting the cloning system and plasmid vector, plasmid restriction digestion, fragment restriction digestion, gel excision, dephosphorylating DNA and more.
FLAG® Tag Bacterial and Mammalian Expression Vectors
FLAG® and 3xFLAG® expression vectors and products for bacterial and mammalian expression vectors, detection and purification of proteins.
SnapFast™ Restriction Site Functions
Learn more about relevant restriction site functions in the SnapFast™ plasmid system. All DNA sections are pre-screened, and where possible modified, to remove any of the restriction sites found within the core SnapFast plasmids to maintain their flexibility.
Mgat4 May Play a Role in Increased Sialylation by Overexpressing Functional MGAT1 in Mgat1-Disrupted Chinese Hamster Ovary (CHO) Cells
MGAT1 adds N-acetylglucosamine to the Man5GlcNAc2 (Man5) structure. Goh et al. reported increased sialylation after restoring MGAT1 function in MGAT1 deficient CHO cells.
Restriction Enzyme Cloning Glossary
Restriction Enzyme Cloning Glossary
Cloning & Expression Vectors
Expression Vectors for Bacterial, Mammalian and Insect Cell Systems. Featured are a variety of tags, promoters and elements for secretion, transient, stable and bicistronic expression.
Plasmid Product Nomenclature
The SnapFast system is a versatile plasmid cloning platform that provides a range of functional DNA sequences in an easy to clone format. Hundreds of pre-designed DNA sections that can be easily incorporated into, or transferred between, our range of
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
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