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应用:Nanoparticle and microparticle synthesis
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Protocols for Membrane Scaffold Proteins and Nanodisc Formation
The following material related to Nanodisc Technology is adapted from on-line content of the research group of Professor Stephen Sligar of the University of Illinois at Urbana-Champaign, with the kind permission of Professor Sligar.
Washing Microparticles
Microparticles protocol for washing particles may be done via centrifugation. This procedure must be performed carefully.
Preparation of Monodisperse Polymer Spheres
Monodisperse, surfactant-free polymer spheres for use as colloidal crystal templates can be easily obtained in reasonably large quantities. Typical synthesis methods for poly(methyl methacrylate) (PMMA) and poly(styrene) (PS) by emulsifier free emulsion polymerization are described below and yield spheres several...