Protein and nucleic acid interactions
Applied Filters:
Applications:Protein and nucleic acid interactions
Content Type:Technical Article
Showing 1-30 of 37 results
使用PEPscreen®多肽研究AAV-hF.IX基因治疗的CTL MHC I类和Th II类免疫应答
重组腺相关病毒(AAV)载体介导的人凝血因子IX(hF.IX)表达基因治疗是一种前景可期的基因转移策略，因其使用肝脏特异性启动子，可实现hF.IX在肝脏中的长期表达和弱免疫应答。但在人类和某些动物中，F.IX表达会随着时间推移而下降，并且载体和转基因会诱发免疫应答。
Duolink®邻位连接检测操作视频
本视频将逐步向您展示如何使用已固定和透化的玻片细胞进行Duolink® PLA邻位检测实验。
Duolink® PLA邻位连接技术在神经科学中的应用
Duolink®邻位连接技术用于研究神经元相互作用，促进神经科学研究。
染色质免疫沉淀（ChIP）试剂盒
获取染色质免疫沉淀 (ChIP) 常见问题的解答，包括超声处理和DNA剪切问题。
优化免疫荧光实验方案获取最优质细胞图像的技巧
免疫荧光法(IF)通过在抗体标记上荧光分子，然后使用激光器激发荧光，包括蛋白质的定位、验证翻译后修饰或活化以及与其他蛋白质的邻近和复合等操作。
RNA免疫沉淀 Chip (RIP) 检测
RNA免疫沉淀（RIP）是一种分析蛋白质与与mRNA、小RNA、病毒RNA或lncRNA相互作用并修饰其功能的基本方法。
Duolink® PLA流式蛋白检测试剂盒
了解有关将邻位连接技术 (PLA) 与流式细胞术相结合的新试剂盒的更多信息，实现了蛋白互作流式分析。
Duolink®邻位连接技术(PLA)多色试剂盒在灵敏的多重蛋白检测中的应用
Duolink® PLA多色技术以及固定组织和细胞中的蛋白互作的定量
邻位连接技术（PLA）的工作原理
了解邻位连接技术的工作原理以及蛋白质互作对照试剂盒如何确定EGF诱导的EGFR-HER2二聚化的原位检测。
利用in situ PLA检测蛋白质间的互作
蛋白质是维持细胞结构和功能所必需的复杂生物分子。大多数蛋白质通常与各种分子（包括其他蛋白质）互作而发挥功能。
如何优化 Duolink® 邻位连接实验
正确地准备、设置和执行 Duolink® PLA 实验方案需要考虑的因素。
Duolink® PLA故障排除指南
包括建议和技巧、常见问题解答和基本故障排除的支持信息。
ChIP: Immunoprecipitation, washing and elution
Chromatin Immunoprecipitation (ChIP), washing and elution
ChIP Troubleshooting Tips
Chromatin Immunoprecipitation (ChIP) Troubleshooting Tips
How Proximity Ligation Assays (PLA) Work
Learn how Proximity Ligation Assay technology works and how the protein-protein interaction control kit can confirm in situ detection of EGF-induced EGFR-HER2 dimerization.
RNA Immunoprecipitation (RIP) FAQs
RNA Immunoprecipitation (RIP) Frequently Asked Questions
ChIC/CUT&RUN Kits
Chromatin Immunocleavage (ChIC) kits and CUT&RUN technology overview for improved chromatin isolation and downstream analyses.
Tips for Optimizing Immunofluorescence Protocols to Get the Best Image
Uses for immunofluorescence (IF)—where an antibody conjugated to a molecule that fluoresces when excited by lasers— include protein localization, confirmation of post-translational modification or activation, and proximity to/complexing with other proteins.
ChIC and CUT&RUN Data Analysis Tool Frequently Asked Questions (FAQs)
Chromatin Immunocleavage (ChIC) and CUT&RUN data analysis tool frequently asked questions (FAQs).
Duolink® PLA Troubleshooting Guide
Support information including tips and tricks, frequently asked questions, and basic troubleshooting.
Detection of Protein-protein Interactions by in situ PLA
Proteins are complex biological molecules essential for cellular structure and functions. The majority of proteins commonly interact with various molecules, including other proteins, in order to exert their functions.
Guides to Cell Number for ChIP and Endpoint Analysis
Guides to cell number for Chromatin Immunoprecipitation (ChIP) and endpoint analysis
Studies of Dimerization and Activation of Receptors in the EGFR-Family Using Duolink® Assays
Our aim was to investigate the dimerization and activation pattern of two receptors, EGFR (ErbB1) and HER2 (ErbB2), in the EGFR-family using breast cancer cell lines and selected colon tissues as model systems.
Duolink® Proximity Ligation Assay (PLA) Multicolor Kits for Sensitive Multiplex Detection
Duolink® PLA Multicolor technology and the quantification of protein-protein interactions in fixed tissues and cells.
RNA Immunoprecipitation Chip (RIP) Assay
RNA Immunoprecipitation (RIP) is an essential method for analyzing proteins that interact with and modify the function of mRNAs, small RNAs, viral RNAs, or lncRNAs.
Duolink® Proximity Ligation Assay Instructional Video
This video will show you step-by-step how to perform a Duolink® PLA experiment with cells on a slide that have already been fixed and permeabilized by the user.
Duolink® PLA Technology for Neuroscience Applications
Duolink® proximity ligation assay used to study neuron interactions furthering neuroscience research.
ChIP-X: Cross-linking proteins to DNA and cell lysis
X-ChIP: Cross-linking proteins to DNA and cell lysis
Fragmentation of chromatin in ChIP
Fragmentation of chromatin in Chromatin Immunoprecipitation (ChIP)
How to Optimize Duolink® PLA for Flow Cytometry Detection
Considerations for proper experimental design, preparation and execution of the Duolink® PLA for flow cytometry protocol.
Page 1 of 2
Page 1 of 2