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Applications:Protein expression
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Duolink® PLA Probemaker指南
Duolink® PLA明场实验方案
本实验方案描述了Duolink® PLA试剂用于组织和细胞样品中个体蛋白质、蛋白质修饰和蛋白质相互作用的明场检测、可视化和定量。
Duolink® 原位精简操作指南 - 荧光
Duolink In Situ后复染实验方案
我们建议在Duolink In Situ 荧光用户手册的第7.3节第5步完成扩增步骤后进行复染操作。
cOmplete™ Protocol
It is possible dissolve 1 tablet in as little as 2 ml double distilled Water, results in a 25x stock solution, without difficulty.
ALiCE®Cell-Free Protein Synthesis System Protocol
Achieve high protein yields with the ALiCE® Cell-Free Protein Synthesis System that allows you to obtain “difficult to produce” proteins in a matter of hours instead of weeks.
Next Generation Cell Free Protein Expression Kit (Wheat Germ) (CFPS700) PROTOCOL
Protein synthesis using cell-free protein synthesis reagent kit consists of three stages: preparation of transcription template, transcription and translation.
Duolink® PLA Brightfield Protocol
This protocol describes the use of Duolink® PLA reagents for the brightfield detection, visualization, and quantification of individual proteins, protein modifications, and protein interactions in tissue and cell samples.
Duolink® In Situ Short Instructions - Fluorescence
This page details the Duolink® In Situ Short Protocol for fluorescence detection
Yale Immunofluorescence Protocol
Procedure for Fixation, Immunostaining, and Imaging in 384-well Plates.
Duolink® PLA Fluorescence Protocol
This protocol describes how to perform immunofluorescent detection of proteins in cells and tissue.
Yeast Growth Protocols
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells. Yeast cultures can be grown, maintained, and stored in liquid media or on agar plates using techniques similar to those for bacterial cultures.
AliCE® Cell-Free Protein Synthesis Kit Quick Start Guide
Follow these five quick steps to perform cell-free protein synthesis using the ALiCE® protein expression plant lysate kit.
Using Duolink® in Protein Interaction Studies
The video follows the simple and straightforward procedure that allows you to detect, quantify and obtain cell localization of protein interactions and their modifications in a single experiment.
Duolink® PLA Probemaker Guide
Guide to create custom PLA probes for immunofluorescent or brightfield detection.
Cancer Research Techniques
By focusing on analysis techniques and protocols specific for Cancer Research, we are putting your research needs first. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Cancer Research.
Duolink® PLA Multicolor Detection Protocol
Duolink® PLA Multicolor Detection Protocol
Handling Inclusion Bodies in Recombinant Protein Expression
How to isolate proteins from inclusion bodies using Cytiva products.
Counterstaining after the Duolink In Situ Protocol
We recommend applying the counterstaining protocol after the completion of the Amplification step in section 7.3, step 5 of the Duolink In Situ Fluorescence User Manual.
Measuring Kinase Inhibitor Residence Times
Jump dilution protocol to determine the residence time of a drug (or drug candidate) during its interaction with a kinase using a fluorescence polarization assay based on the detection of ADP.
Duolink® PLA Flow Cytometry Protocol
Protocol for use of Duolink® PLA reagents for the detection of individual proteins, protein modifications, and protein-protein interactions within cell populations by flow cytometry.
How to Create PLA® Probes
Duolink® kits use in situ PLA®, a proximity ligation assay technology, to accurately and objectively quantify individual proteins, and their interactions and modifications in unmodified cells and tissue.
Yeast Transformation Protocols
Yeasts are considered model systems for eukaryotic studies as they exhibit fast growth and have dispersed cells.
Liberase™ TM Research Grade Protocol & Troubleshooting
Liberase™ TM Research Grade Protocol & Troubleshooting