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应用:Protein purification
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用于亲和层析的色谱柱填充和制备实验方案
该页面展示了如何使用GE医疗所提供的Tricorn™或XK色谱柱进行用于亲和层析的色谱柱填充和制备。
使用Superdex进行分离
来自Cytiva医疗的Superdex是由葡聚糖和琼脂糖的复合基础基质组成的SEC介质。本页面介绍如何使用superdex执行分离任务。
使用Sephacryl®进行分离
本页介绍如何使用Cytiva医疗的sephacryl色谱柱进行分离,该色谱柱是通过将烯丙基葡聚糖与N,N'-亚甲基双丙烯酰胺共价交联而制备的尺寸排阻色谱介质。
亲和层析缓冲液置换和脱盐
如何使用Sephadex G-25、HiTrap脱盐柱或ÄKTAprime plus进行缓冲液置换和脱盐。
Performing a Purification of IgG Antibodies with Protein G Sepharose® 4 Fast Flow
This page shows how to separate IgG antibodies by affinity chromatography using Protein G Sepharose 4 Fast Flow from Cytiva.
Purification or Removal of Albumin
This page shows how purify or remove albumin with Blue Sepharose and Capto Blue from Cytiva.
Performing a Separation with HiTrap® IgM Purifcation HP
How to perform a separation with the HiTrap® IgM Purifcation HP from Cytiva which is an Affinity Chromatography product optimized for purification of monoclonal IgM from hybridoma cell culture.
Sample Preparation for Affinity Chromatography
This page shows how to prepare samples for purification with affinity chromatography.
Performing a Purification of IgG Antibodies with Protein A Mag Sepharose® Xtra or Protein G Mag Sepharose® Xtra
Protein A Mag Sepharose® Xtra and Protein G Mag Sepharose® Xtra products are magnetic beads designed for high capacity small-scale purification/screening of monoclonal and polyclonal antibodies from various species.
Sample Preparation for Chromatographic Purification
This page discusses various aspects of sample preparation for chromatographic purification.
Selectivity and the Properties of a HIC Medium
Selectivity of HIC medium from Cytiva is based on properties such as hydrophobicity, porosity and particle size.
Refolding using ion exchange chromatography and analysis of refolding
This page shows how to refold proteins using ion exchange chromatography with prepacked ion exchange chromatography columns from Cytiva and how to analyze the refolding process with Superdex columns from Cytiva.
Purification using Glutathione Sepharose® High Performance, Glutathione Sepharose® 4 Fast Flow, and Glutathione Sepharose® 4B
This page shows how to purify GST-tagged proteins using Glutathione Sepharose from Cytiva.
Performing a Separation of Fibronectin with Gelatin Sepharose® 4B
This page shows how to separate fibronectin by affinity chromatography using Gelatin SepharosePerforming a Separation of Fibronectin with Gelatin Sepharose® 4B from Cytiva.
Manual Purification Using HisTrap FF Crude Kit with a Syringe
HisTrap™ FF crude Kit is designed for rapid and convenient manual purification of histidine-tagged proteins using premade buffers and a syringe. Histidine-tagged proteins can be purified directly from unclarified cell lysates. This saves time because the pretreatment of the sample...
Minipreps using His SpinTrap™ and His SpinTrap™ Kit
This page describes a small-scale purification (minipreps) of histidine-tagged recombinant proteins using His SpinTrap and His SpinTrap Kit from Cytiva.
Magnetic Bead-Based Purification/Screening Using His Mag Sepharose® Ni
This page shows magnetic bead-based purification and screening using His Mag Sepharose Ni from Cytiva.
Purification of Histidine-Tagged Recombinant Proteins Using Ni Sepharose 6 Fast Flow
This page shows how to purify histidine-tagged recombinant proteins using Ni Sepharose 6 Fast Flow from Cytiva. Ni Sepharose 6 Fast Flow consists of 90 µm beads of highly cross-linked agarose, to which a chelating ligand has been immobilized and...
Sera-Mag™ SpeedBeads Magnetic Protein A/G-Particles Protocol
Sera-Mag SpeedBeads Protein A/G Magnetic Particles provide a fast and convenient method for both manual and automated magnetic isolation of proteins using affinity binding. The particles can be used for isolating antibodies from serum, cell culture supernatant or ascites, and...
Performing a Separation with Cytiva Products Based on Poly (His) Fusion Proteins
This page shows how to perform a separation with poly (His) fusion proteins which are affinity chromatography tags used in His MicroSpin Purification Module, HisTrap™ Kit, HiTrap® Chelating HP and Chelating Sepharose® Fast Flow products from Cytiva.
Performing a Separation with Sephacryl®
This page shows how to perform a separation with a sephacryl column from Cytiva which are Size Exclusion Chromatography media prepared by covalently cross-linking allyl dextran with N,N’-methylene bisacrylamide.
Purification or Removal of Glycoproteins and Polysaccharides
This page shows how to purify or remove glycoproteins and polysaccharides with Con A Sepharose 4B, Lentil Lectin Sepharose 4B, Capto Lentil Lectin from Cytiva.
Clean-up of Antibody Labeling Reactions Using Amicon Ultra Filters
Using a standard Amicon® Ultra centrifugal filter, a few rounds of diafiltration can efficiently remove unincorporated label from an antibody labeling reaction without diluting the sample.
MonoBeads: Purification of Milligram Quantities with Highest Resolution
This page covers using Monobeads for purification of proteins, peptides, or oligonucleotides.
Sepharose Big Beads: Purification from Crude, Viscous Samples at Large Scale
This page covers how and why to use Sepharose Big Beads for purification from crude, viscous samples.
Purification from Unclarified Cell Lysate using HisTrap™ FF Crude
This page shows how to purify histidine-tagged recombinant proteins from unclarified cell lysate using HisTrap FF crude from Cytiva.
Performing a Purification of NAD+-dependent dehydrogenases and ATP-dependent kinases with 5’ AMP Sepharose® 4B, HiTrap® Blue HP and Blue Sepharose® 6 Fast Flow
This page shows how to purify NAD+-dependent dehydrogenases and ATP-dependent kinases by affinity chromatography using 5’ AMP Sepharose® 4B, HiTrap® Blue HP and Blue Sepharose® 6 Fast Flow products from Cytiva.
Immunoprecipitation of FLAG Fusion Proteins Using Monoclonal Antibody Affinity Gels
Protocol for immunoprecipitation (IP) of FLAG fusion proteins using M2 monoclonal antibody 4% agarose affinity gels
Purification of Histidine-Tagged Proteins using HiTrap® IMAC HP and HiTrap® IMAC FF
This page shows how to purify histidine-tagged proteins using HiTrap® IMAC HP and HiTrap® IMAC FF from Cytiva.
Performing a Separation with Superdex
Superdex from Cytiva are SEC media consisting of a composite base matrix of dextran and agarose. This page shows how to perform a separation with a superdex column.
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