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色谱柱填充和抗体亲和层析的制备
本页介绍了高效的色谱柱填充和抗体亲和层析的制备。
抗体亲和色谱的脱盐和缓冲液置换
实验室规模的脱盐是一种成熟、简单、快速的方法,可以在将样品转移至所需缓冲液的同时快速去除低分子量污染物。
Manual Purification of Histidine-Tagged Proteins using His GraviTrap™ TALON® Columns
His GraviTrap™ TALON® columns are prepacked with 1 ml of TALON® Superflow medium. Each column provides simple manual purification of up to 15 mg of histidine-tagged proteins.
Optimizing Purification of Histidine-Tagged Proteins
How to optimize purification of histidine-tagged proteins using Cytiva products.
Principles and Standard Conditions for Different Purification Techniques
This page describes principles and standard conditions for different purification techniques of histidine-tagged proteins using Cytiva products.
Performing a Separation with IgG Sepharose 6 Fast Flow
Perform a separation with IgG Sepharose 6 Fast Flow from Cytiva, an Affinity Chromatography product for purification of recombinant fusion proteins containing a protein A tail.
Chelators
BioUltra Chelators. A chelate is a water-soluble complex between a metal ion and a complexing agent. It usually does not dissociate easily in solution, but forms an inert complex.
Column, Media and Sample Preparation for Hydrophobic Interaction Chomatography
Here the correct ways for Column, Media and Sample preparation for Hydrophobic Interaction Chomatography (HIC) with Cytiva media are detailed, including sample application, load volume and temperature
Column Packing and Preparation for Affinity Chromatography of Antibodies
This page describes efficient column packing and preparation for affinity chromatography of antibodies.
Cleavage and Purification of GST-Tagged Protein Bound to GSTrap
This page shows how to cleave and purify GST-tagged proteins bound to GSTrap from Cytiva.
Troubleshooting Purification Methods
This page shows troubleshooting methods for purification of GST-tagged proteins using Cytiva products.
Performing High-Throughput Screening Using His MultiTrap™ HP and His MultiTrap™ FF 96-Well filter Plates
This page shows high-throughput screening using His MultiTrap™ HP and His MultiTrap™ FF 96-well filter plates from Cytiva.
IgG Antibody Purification with HiTrap® Protein G HP Columns
This page shows how to separate IgG antibodies by affinity chromatography using HiTrap® Protein G HP columns from Cytiva.
Characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (High Sub) Media and Columns
This page describes characteristics of Glutathione Sepharose and HiTrap® Benzamidine FF (high sub) media and columns from Cytiva.
Purification of Membrane Proteins
This page shows how to perform a purification of His-tagged membrane proteins.
Performing a Purification of IgM Antibodies with HiTrap™ IgM Purification HP
This page shows how to purify IgM antibodies by affinity chromatography using HiTrap™ IgM Purification HP.
Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells
Centrifugation-free Protein Extraction and Purification from Total Cultures of Baculovirus-infected Insect Cells
Purification Using Protein A-based Chromatography Media
This page shows various purification options for Protein A Sepharose chromatography media and describes typical binding and elution conditions for Protein A Sepharose chromatography media.
Troubleshooting Strategies in GST-tagged Protein Purification
This page describes troubleshooting strategies in purification of GST-tagged proteins using Cytiva products.
Manual and Automated Purification
This page describes manual and automated purification of histidine-tagged recombinant proteins using Cytiva products
Purification or Removal of Proteins and Peptides
This page shows how to purify or remove proteins and peptides with exposed amino acids with Chelating Sepharose High Performance, Chelating Sepharose Fast Flow, Capto Chelating from Cytiva.
Performing a Purification of IgG Antibodies with Protein G GraviTrap™
This page shows how to separate IgG antibodies by affinity chromatography using Protein G GraviTrap from Cytiva.
Purifcation or Removal of Serine Proteases, e.g. Thrombin and Trypsin, and Zymogens
This page shows how to remove serine proteases, e.g. thrombin and trypsin, and zymogens from recombinant products using HiTrap Benzamidine FF and Benzamidine Sepharose 4 Fast Flow from Cytiva.
Performing a Separation with HiTrap® Protein G HP
Purify monoclonal or polyclonal IgG from serum, cell culture supernatant or ascitic fluid using the HiTrap Protein G HP from Cytiva, an affinity-exclusion chromatography product containing Sepharose-immobilized Protein G.
Cleavage and Purification of GST-Tagged Protein Eluted from GSTrap
This page shows how to cleave and purify GST-tagged proteins eluted from GSTrap from Cytiva.
Troubleshooting Methods for Purification of MBP-Tagged Recombinant Proteins
Troubleshooting of methods for purification of MBP-tagged recombinant proteins using Cytiva products.
Purification or Removal of Serine Proteases, such as Thrombin and Trypsin, and Zymogens
This page shows how to Purify or remove serine proteases with a Benzamidine Sepharose 4 Fast Flow, Benzamidine Sepharose 4 Fast Flow from Cytiva.
General Considerations for Purification of GST-tagged Protein
The yield of GST-tagged proteins is highly variable, ranging from 1 mg/l to 10 mg/l. The yield depends on various parameters, such as nature of the tagged protein, the host cell, and the culture conditions used.
Capto MMC
Capto MMC is a multimodal cation exchanger with the properties of a weak cation exchanger. In addition to electrostatic interactions, the ligand structure provides for additional interaction modes such as hydrophobic interaction, hydrogen bonding, and thiophilic interaction.
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