Stem cell culture

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3D多能干细胞：简化的培养和实际分化

该白皮书阐述了三维（3D）细胞培养在生成多能干细胞（PSC）衍生的胚胎发育和分化模型中的重要性。它包括了对胚状体（EB）的一般概述，以及对生成胚状体的各种方法之间的比较。

胚胎培养基

用于转基因小鼠胚胎培养的小鼠胚胎培养基及经过胚胎培养验证的试剂

新型无异种和无血清成骨分化培养基中人间充质干细胞的加速骨细胞分化

新型无血清、无异种人间充质干细胞（MSC）骨细胞分化培养基的开发。

癌症干细胞：癌症治疗的靶标

培养和表征CSC群体的癌症干细胞培养基、球状体培养板和癌症干细胞标志物。

诱导多能干细胞分化方案

我们可提供大量用于控制人类iPSC细胞命运的细胞培养基、补充剂、生物活性小分子和生长因子。

Hematopoietic Cytokines

Read article on hematopoietic cytokines and hematopoiesis

Derivation of Functional Oligodendrocyte Progenitor Cells (OPCs) from Human Neural Stem Cell Lines

Derivation and characterization of functional human neural stem cell derived oligodendrocyte progenitor cells (OPCs) that efficiently myelinate primary neurons in culture.

Mesenchymal Stem Cell Culture

Highly characterized human mesenchymal stem cell lines from bone-marrow, adipose and umbilical cord along with serum-free MSC expansion and differentiation media.

Cancer Stem Cells: Targets for Cancer Therapy

Cancer stem cell media, spheroid plates and cancer stem cell markers to culture and characterize CSC populations.

IPS Cells or Induced Pluripotent Stem Cell FAQs

FAQs and information about IPS Cells or induced pluripotent stem cells (iPSCs). Including what are IPS cells, learning stem cell basics, reprogramming methods, and iPS cell culture techniques.

Pluripotent and Multipotent Stem Cells

Read types of stem cells including multipotent stem cells, Pluripotent stem cells and iPSCs and their applications in basic stem cell research, stem cell therapy and disease modelling

Growth Factors in Stem Cell Biology

Role of growth factors in stem cell differentiation and various growth factors for your research at sigmaaldrich.com

ReNcell® Human Neural Progenitors: Renewable and Consistent Human Neural Stem Cell Lines that Differentiate into Functional Neurons

ReNcell neural progenitors are immortalized human neural stem cell lines that can differentiate into neurons, astrocytes sand oligodendrocytes.

Neuronal Mesenchymal Stem Cells

Protocol to differentiate human mesenchymal stem cells into neurons with extensive dendrites and axons in 3 days. Explore over 350 PromoCell products on Sigma.com.

Canonical Wnt / β-Catenin Signaling

We review the Wnt signaling pathway (also, canonical Wnt or beta (β)-catenin pathway) and provide links to related products at sigmaaldrich.com.

Neural Stem Cell FAQs

Frequently asked questions about neural stem cells including NSC derivation, expansion and differentiation.

A Serum-Free and Feeder-Free Defined Medium That Enables Weekend-Free Culture of Undifferentiated Human ES/iPS Cells

Skip weekend feedings. Defined serum-free and feeder-free expansion media for human pluripotent stem cells (ES and iPS cells). See publications and protocols.

Robust Differentiation of Human iPSCs into Lineage-Specific Neurons and Glial Cells Utilizing Dual-SMAD Inhibition

Human iPSC neural differentiation media and protocols used to generate neural stem cells, neurons and glial cell types.

Water for Mammalian Cell Culture

Ensure optimal cell culture outcomes with ultrapure, endotoxin-free water. Learn about the impact of water impurities on mammalian cell cultures and how to achieve optimal cell growth.

3D Pluripotent Stem Cells: Culture & Differentiation

3D cell culture enhances pluripotent stem cell models of embryo development, providing insights into differentiation.

PLTMax® Human Platelet Lysate (hPL): A Superior Serum-Free Media Supplement for Robust Mesenchymal Stem Cell Expansion

PLTMax® Human Platelet Lysate (hPL) is a superior serum-free and xeno-free media supplement alternative to fetal bovine serum (FBS) for human mesenchymal stem cell (MSC) cultures.

Leukemia Inhibitory Factor (LIF) Protein

Leukemia Inhibitory Factor (LIF) is a stem cell growth factor used for the in vitro culture of pluripotent mouse embryonic stem cells (ES cells).

Accelerated Osteocyte Differentiation of Human Mesenchymal Stem Cells in a Novel Xeno-free and Serum-free Osteogenesis Differentiation Media

Development of a novel serum-free and xeno-free human mesenchymal stem cell (MSC) osteocyte differentiation media.

Single Transfection of a Synthetic Polycistronic Self-replicative RNA Yields High Numbers of Transgene-free Human iPSCs

The Simplicon™ RNA Reprogramming Technology is a next generation reprogramming system that uses a single synthetic, polycistronic self-replicating RNA strand engineered to mimic cellular RNA to generate human iPS cells.

Embryo Culture Media

Mouse embryo media and embryo validated reagents for transgenic mouse embryo culture

Mouse Embryonic Fibroblasts (MEF Feeder Cells)

Mouse embryonic fibroblasts (MEFs) serve as a feeder layer for both mouse and human embryonic stem cells (ES cells) and induced pluripotent stem cells (iPSCs).

Mesenchymal Stem Cell FAQs

Frequently asked questions about mesenchymal stem cells including MSC derivation, expansion, differentiation and allogenic stem cell therapy.

ECMatrix™ Laminin Substrates are Superior Xeno-free Extracellular Matrix Coatings for Feeder-free Human Pluripotent Stem Cell Cultures

No precoat needed. Single cell iPSC passaging. Human recombinant laminin-511 E8 allows greater iPS cell adhesion and proliferation rates vs. Matrigel and laminin-521.

Cancer Stem Cell Detection with AldeRed™ 588-A

AldeRed™ 588-A detects ALDH activity in cancer stem cells, aiding in cancer research.

HumanKine® Thermostable bFGF

Fibroblast growth factors (FGFs) regulate developmental pathways and mesoderm/ectoderm patterning in early embryonic development.

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