Transfection and gene editing

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facet applications:Transfection and gene editing

facet content type:Protocol

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Procedure for Preparation of Cationic Lipid/Oligomer Complex - Avanti Research™

Find a detailed protocol for preparing transfection reagents and optimizing gene editing techniques in cell culture, ensuring effective results.

Calcium Phosphate Transfection Kit Protocol

Calcium phosphate transfection is a common method for the introduction of DNA into eukaryotic cells. This protocol can be optimized for use with a wide variety of cell types.

Escort™ IV Transfection Reagent Protocol

Product manual provides detailed protocol for easy DNA transfection.

Escort™ III Transfection Reagent Protocol

The product bulletin providin detailed use protocol for easy DNA transfection.

Liposome Preparation - Avanti® Polar Lipids

Lipid vesicle preparation involves hydration and sizing steps, applicable regardless of lipid composition.

Preparation of Cationic Liposomes & Transfection of Cells - Avanti^® Polar Lipids

This procedure describes the preparation of cationic liposomes and transfection of cells.

Preparation of Cationic Liposomes & Transfection of Cells - Avanti® Polar Lipids

This procedure describes the Preparation of Cationic Liposomes & Transfection of Cells

Preparation of Competent Cells and Transformation with pGEX DNA

This page describes preparation of competent cells and transformation with pGEX DNA.

Protocol for Transducing Mouse Embryonic Fibroblasts (MEF) using MISSION® ExpressMag™ Super Magnetic Kit®

This detailed procedure allows you to transduce Mouse Embryonic Fibroblasts (MEF) using MISSION ExpressMag Super Magnetic Kit.

Universal Transfection Reagent Protocol

Universal Transfection Reagent enables efficient nucleic acid delivery into various cells, compatible with different cell culture conditions.

X-tremeGENE™ siRNA Transfection Reagent Protocol & Troubleshooting

Protocols for Transfecting Common Cell Lines with X-tremeGENE™ Transfection Reagents

Co-transfection of Plasmid DNA

Transient co-transfection of plasmids for cellular studies in protein interaction, transcription factor, and gene knockdown analyses.

X-tremeGENE™ 9 DNA Transfection Reagent Protocol

Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

X-tremeGENE™ HP DNA Transfection Reagent Protocol

Cell preparation for transfection Plate cells approx. 24 hours before transfection making sure cells are at optimal concentration (70 – 90 % confluency).

Lentiviral Production Using X-tremeGENE HP Transfection Reagent

Lentiviruses represent a powerful tool in research applications to transduce a wide range of cell types.

Yeast Transformation Kit

The selection of plasmids in yeast is based on the use of auxotrophic mutant strains, which cannot grow without a specific medium component (an amino acid, purine, or pyrimidine)

CRISPR Lentiviral Screening: Antibiotic Selection

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

Genome Editing in Plants with CRISPR/Cas9

ZFNs and CRISPR/Cas9 advance targeted genome editing, opening new research avenues.

Lentiviral Transduction Protocol

Detailed procedure for how to perform a lentiviral transduction of MISSION shRNA lentiviral particles to achieve a stable long term silencing and phenotypic change.

Simplicon™ RNA Transfection and Electroporation Protocols

FACS Titration of Lentivirus Protocol

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

磷酸钙转染试剂盒实验方案

使用磷酸钙将DNA转入真核细胞是一种常见的转染方法。该转染实验方案可经优化用于各种细胞类型。

脂质体制备 - Avanti® Polar Lipids

脂质配方的性质取决于具体成分（阳离子、阴离子和中性脂质）。但是，同一种制备方法适用于所有成分的脂质囊泡。制备流程的一般要素包括制备用于水合的脂质、搅拌水合以及减小粒径，从而获得均匀分布的囊泡。

利用X-tremeGENE™ 转染试剂转染常见细胞系的实验方案

X-tremeGENE™ 9 DNA转染试剂实验方案

约转染前24小时接种细胞，确保细胞处于最佳密度（7090 %融合度）。

X-tremeGENE™ HP DNA转染试剂实验方案

约转染前24小时接种转染细胞，确保细胞处于最佳密度（7090 %融合度）。

使用X-tremeGENE HP转染试剂进行慢病毒生产

慢病毒是一种用于在研究应用中转染多种细胞类型的强大工具。

植物CRISPR/Cas9基因组编辑

锌指核酸酶（ZFN）是由Sigma-Aldrich在不到8年前推出的，但是从那时起靶向基因组编辑技术已迅速发展。最近，CRISPR/Cas9 通路的发现加快了对该领域的兴趣，为研发开辟了新的可能性。

慢病毒转导实验方案

如何利用MISSION shRNA慢病毒颗粒进行慢病毒转导，以实现稳定的长期沉默和表型变化的详细操作步骤。