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Transfection and gene editing
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facet applications:Transfection and gene editing
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利用慢病毒成功转导
获取处理慢病毒、优化实验设置、测定慢病毒颗粒滴度以及选择实用转导产品方面的贴士。
分子克隆的问题解决
分子克隆是将目的基因 (GOI) 插入到质粒载体中的过程，然后将该载体插入到表达 GOI 编码的蛋白质的细胞中。一旦蛋白质在细胞中得到表达，这种表达即可用于各种不同的研究（如细胞信号传导、形态学或其他方面）。
慢病毒转导
在这个实验中，我们将展示两种不同细胞系上的慢病毒转导。
抗生素杀灭曲线
抗生素杀灭曲线是一项剂量响应实验，通过逐渐增大施加在哺乳动物细胞中的备选抗生素剂量进行测定。
细胞转染简介
转染是将DNA、RNA或蛋白质引入真核细胞的过程，用于研究和调节基因表达。因此，转染技术和实验方案作为分析工具，有助于表征遗传功能、蛋白质合成、细胞生长和发育。
Detect, Visualize and Quantify Single Post-Translational Modifications
Sigma-Aldrich presents a Biofiles on Detect, Visualize and Quantify Single Post-Translational Modifications
Functionalized PEI and Its Role in Gene Therapy
Gene therapy has become one of the most discussed techniques in biomedical research in recent years.
Development of a New Advanced KSOM Mouse Embryo Media that Enables Dual-Use Embryo Culture and Cell Handling Applications
Advanced KSOM mouse embryo media that can be used as a single medium for both harvest and culture of mouse embryos to facilitate creation of transgenic knockout mice.
Lentiviral Transduction
In this exercise we will be demonstrating lentiviral transduction on two different cell lines.
siRNA Design, Manufacturing, Transfection & Detection
RNA interference (RNAi) is a natural biological mechanism wherein short inhibitory RNA (siRNA) duplexes induce potent inhibition of gene expression.
siRNA Delivery in Mammalian Cell Lines
siRNAs are effectively delivered into mammalian cell lines that have been difficult or even impossible to transfect with siRNA when using other commercially available lipofection reagents.
Whole Genome Amplification for Single Cell Biology
Whole genome amplification overcomes restrictions for single-cell genomic analyses with non-specific amplification.
Antibiotic Kill Curve
Antibiotic kill curve is a dose response experiment in which mammalian cells are subjected to increasing amounts of selection antibiotic
Why Use Antibiotics in Cell Culture?
Know when to use antibiotics to prevent bacterial or fungal, mycoplasma, or viral contamination in cell culture and find suitable antibiotics or other biological agents.
Introduction to Cell Transfection
Transfection introduces genetic material into cells, aiding research in gene expression and cell biology.
Validation of RNAi Knockdown Using Multiple Reaction Monitoring and Protein-AQUA
This discussion will highlight some of these methodologies, namely, the use of Multiple Reaction Monitoring (MRM) and Protein-AQUA.
X-tremeGENE™ siRNA Transfection Reagent
Small inhibitory RNAs offer easy gene expression knockdown in mammalian cells, revolutionizing gene research.
Reverse Transfection of Plasmid DNA
Automation is used for many applications to reduce variation caused by manual handling and to obtain reproducible results in high-throughput assays. High-throughput applications, such as knockdown studies or target screenings, often include cell transfection.
Methods for Enhancing Lentiviral Transduction Efficiency
Methods for lentiviral transduction of Jurkat cells were compared. Spinoculation was compared with overnight incubation with polybrene (hexadimethrine bromide) and fibronection-coated plates.
Successful Transduction Using Lentivirus
Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.
Cloning Troubleshooting
Molecular cloning inserts genes into plasmid vectors for protein expression in different studies.