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Dragana Stanic-Vucinic et al.
Molecular nutrition & food research, 56(12), 1894-1905 (2012-10-16)
The aim of this work was to investigate the effects of high-intensity ultrasound (sonication), on the structure and allergenicity of the major cow's milk allergen, beta-lactoglobulin (BLG). Structural changes upon sonication of BLG were monitored by circular dichroism spectroscopy, tryptophan
In vitro refolding of porcine pepsin immobilized on agarose beads
Kurimoto, E., et al.
The Journal of Biological Chemistry, 130, 295-297 (2001)
Olli Laine et al.
Journal of molecular biology, 381(1), 89-101 (2008-07-01)
The biotin repressor is an allosterically regulated, site-specific DNA-binding protein. Binding of the small ligand bio-5'-AMP activates repressor dimerization, which is a prerequisite to DNA binding. Multiple disorder-to-order transitions, some of which are known to be important for the functional
Gastric proteinases--structure, function, evolution and mechanism of action.
B Foltmann
Essays in biochemistry, 17, 52-84 (1981-01-01)
Noor Akmal Syed Husain Sharifah et al.
Methods in molecular biology (Clifton, N.J.), 952, 187-196 (2012-10-27)
Fluorescence in situ hybridization (FISH) is increasingly gaining importance in clinical diagnostics settings. Due to the ability of the technique to detect chromosomal abnormalities in samples with low cellularity or containing a mixed population of cells even at a single-cell
Qiang Zou et al.
Journal of food science, 77(5), M270-M277 (2012-11-21)
Bifidobacterium bifidum F-35 was microencapsulated into whey protein microcapsules (WPMs) by a transglutaminase (TGase)-induced method after optimization of gelation conditions. The performance of these WPMs was compared with that produced by a spray drying method (WPMs-A). WPMs produced by the
Sophia P Pilipchuk et al.
Journal of biomedical materials research. Part A, 101(10), 2883-2895 (2013-03-19)
Natural hydrogels have been investigated for three-dimensional tissue reconstruction and regeneration given their ability to emulate the structural complexity of multi-component extracellular matrices (ECM). Hydrogels rich in ECM can be extracted and assembled from soft tissues, retain a composition specific
Marilyn Schallom et al.
American journal of critical care : an official publication, American Association of Critical-Care Nurses, 22(5), 408-411 (2013-09-03)
Because reflux of gastric juice into the oropharynx must precede its aspiration into the lungs, it is reasonable to hypothesize that the detection of pepsin (the major gastric enzyme in gastric juice) in oral secretions may provide a relatively noninvasive
Hana Dvořáková et al.
Frontiers in cellular and infection microbiology, 10, 66-66 (2020-03-17)
Schistosomula (the post-infective stages) of the neurotropic schistosome Trichobilharzia regenti possess multiple isoforms of cathepsin B1 peptidase (TrCB1.1-TrCB1.6) with involvement in nutrient digestion. The comparison of substrate preferences of TrCB1.1 and TrCB1.4 showed that TrCB1.4 had a very narrow substrate
The use of agarose-bound pepsin for the preparation of F(ab')2 fragments of IgG.
J H Beeson et al.
Immunochemistry, 14(4), 305-312 (1977-04-01)
Widya A Wahab et al.
International journal of experimental pathology, 97(4), 303-309 (2016-09-24)
Coeliac disease (CD) is an inflammatory disorder of the small intestine. It includes aberrant adaptive immunity with presentation of CD toxic gluten peptides by HLA-DQ2 or DQ8 molecules to gluten-sensitive T cells. A ω-gliadin/C-hordein peptide (QPFPQPEQPFPW) and a rye-derived secalin
Testing for laryngopharyngeal reflux in exhaled breath condensate: a pilot study.
C R Davies-Husband et al.
Clinical otolaryngology : official journal of ENT-UK ; official journal of Netherlands Society for Oto-Rhino-Laryngology & Cervico-Facial Surgery, 37(6), 497-498 (2012-12-21)
Utsav P Tiwari et al.
Animal science journal = Nihon chikusan Gakkaiho, 88(8), 1093-1099 (2016-11-24)
The highly variable cost and limited availability of conventional feedstuffs make it imperative to explore alternative feedstuffs to be used in swine. Macadamia nut cake (MNC), a coproduct of the macadamia nut oil industry, has not been well studied. MNC
Mahmoud-Awny Magdy et al.
European journal of pharmacology, 697(1-3), 126-131 (2012-10-12)
Ample of evidence proved the gastroprotective effect of thymoquinone (TQ), the main constituent of Nigella sativa oil; however, the full mechanistic cassette on the gastric ulcer etiopathogenesis is not fully elucidated. The aim of the present work is to unveil
J Pohl et al.
Biochemistry, 27(13), 4827-4834 (1988-06-28)
The possibility that pig pepsin has a cation binding specificity in its secondary binding subsites has been examined by the pepsin-catalyzed hydrolysis of a series of synthetic octa- to undecapeptide substrates. These chromophoric substrates are cleaved by pepsin in the
Shenlin Wang et al.
The Journal of biological chemistry, 289(2), 697-707 (2013-11-23)
Multidomain protein folding is often more complex than a two-state process, which leads to the spontaneous folding of the native state. Pepsin, a zymogen-derived enzyme, without its prosegment (PS), is irreversibly denatured and folds to a thermodynamically stable, non-native conformation
Zhen-Hai Zhang et al.
Journal of biomedical nanotechnology, 9(4), 601-609 (2013-04-30)
N-octyl-N-Arginine chitosan (OACS) was synthesized in an attempt to combine the permeation enhancing effects of arginine-rich peptides and the drug loading capacity of the amphipathic polymers for insulin oral delivery. OACS self-assembled micelles of insulin were prepared by the conventional
Susette Audet et al.
The Journal of infectious diseases, 194(6), 781-789 (2006-08-31)
Measles infection induces lifelong immunity; however, wild-type infection stimulates higher levels of measles-virus-neutralizing antibodies (mnAbs) than does vaccination. Because the proportion of the donor population with vaccine-induced measles immunity is increasing, this study was conducted to determine whether this shift
Ilhan Inci et al.
The Journal of surgical research, 184(2), 1143-1149 (2013-05-28)
We tested whether an injured lung graft from category-3 donation after cardiac death donor could be reconditioned with an ex vivo lung perfusion (EVLP) system by intrabronchial diluted surfactant lavage before transplantation. In a pig model, cardiac arrest was induced
Beatriz Cabanillas et al.
Molecular nutrition & food research, 56(12), 1884-1893 (2012-10-20)
The aims of this study were to evaluate IgE-mediated hypersensitivity to pine nut with details of clinical reactions and to characterize major pine nut allergens. The study included ten consecutive teenagers and adults diagnosed with IgE-mediated clinical allergy to pine
Ibrahim Al-Abdulla et al.
Journal of immunological methods, 402(1-2), 15-22 (2013-11-20)
Antivenoms are typically produced in horses or sheep and often purified using salt precipitation of immunoglobulins or F(ab')2 fragments. Caprylic (octanoic) acid fractionation of antiserum has the advantage of not precipitating the desired antibodies, thereby avoiding potential degradation that can
Christie McCracken et al.
Chemical research in toxicology, 26(10), 1514-1525 (2013-09-14)
Toxicity of commercial nanoparticles of titania, silica, and zinc oxides is being investigated in this in vitro study. Particles of these compositions are found in many food items, and thus this study is directed toward particle behavior in simulated digestion
D Lee et al.
The Biochemical journal, 104(3), 742-748 (1967-09-01)
Methods are described for the isolation and purification of pepsin D, an enzyme which accounts for about 10% of the enzymic activity in commercial preparations of pepsin. Pepsin D is similar to pepsin in having a molecular weight of about
Eun-Kyung Kim et al.
Journal of microbiology and biotechnology, 22(10), 1381-1387 (2012-10-19)
We applied enzymatic hydrolysis and tangential flow filtration (TFF) to purify a novel anticancer peptide from Mytilus coruscus (M. coruscus) and investigated its anticancer properties. To prepare the peptide, eight proteases were employed for enzymatic hydrolysis. Pepsin hydrolysates, which showed
Mohamed Mohamed Abdel-aziz et al.
International journal of pediatric otorhinolaryngology, 77(10), 1765-1770 (2013-09-10)
We sought to evaluate the clinical role of pepsin for laryngopharyngeal reflux (LPR) in children with otitis media with effusion (OME). Pepsin/pepsinogen and fibrinogen were analyzed in fifty effusion and blood samples of children with OME using enzyme linked immunosorbent
R A Rocha et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 59, 104-110 (2013-06-12)
Fluoride is considered important for health because of its beneficial effect on the prevention of dental caries and on bone development in the child population. However, excessive intake has negative effects. The main pathway for exposure is oral, through consumption
Digested Ara h 1 has sensitizing capacity in Brown Norway rats
Bogh, K.L., et al.
Clinical and Experimental Allergy, 39(10), 1611-1621 (2010)
Moushree Pal Roy et al.
Indian journal of biochemistry & biophysics, 49(4), 266-271 (2012-10-20)
Phytases catalyze the release of phosphate from phytic acid. In this study, a phytase producing bacterial strain Shigella sp. CD2 was isolated from the wheat rhizosphere. Phytase production started from the exponential phase of bacterial growth, showing the highest activity
Ludmila Tucková et al.
Journal of leukocyte biology, 71(4), 625-631 (2002-04-03)
Celiac disease, induced by dietary gluten, is characterized by mucosal atrophy and local inflammation associated with cell infiltration and activation. Unlike other food proteins, gluten and its proteolytic fragments, besides inducing a specific immune response, were shown to activate components
María Llarena-Reino et al.
Veterinary parasitology, 191(3-4), 276-283 (2012-10-10)
During the last 50 years human anisakiasis has been rising while parasites have increased their prevalence at determined fisheries becoming an emergent major public health problem. Although artificial enzymatic digestion procedure by CODEX (STAN 244-2004: standard for salted Atlantic herring
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