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[The use of single-dose total body irradiation before bone marrow transplantation in treatment of leukemia].
H Tang
Zhonghua fang she xue za zhi Chinese journal of radiology, 16(4), 296-300 (1982-11-01)
Vivek Kumar Morya et al.
Applied biochemistry and biotechnology, 166(1), 243-257 (2011-11-11)
A total of 49 protein sequences of alkaline proteases retrieved from GenBank representing different species of Aspergillus have been characterized for various physiochemical properties, homology search, multiple sequence alignment, motif, and super family search and phylogenetic tree construction. The sequence...
S M Krieger et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 59, 165-176 (2013-06-12)
There remains a need for a simple and predictive animal model to identify potential respiratory sensitizers. The mouse intranasal test (MINT) was developed to assess the relative allergic potential of detergent enzymes, however, the experimental endpoints were limited to evaluation...
Karina Jiménez-Durán et al.
Plant physiology, 161(1), 97-107 (2012-11-15)
In Solanaceae, the self-incompatibility S-RNase and S-locus F-box interactions define self-pollen recognition and rejection in an S-specific manner. This interaction triggers a cascade of events involving other gene products unlinked to the S-locus that are crucial to the self-incompatibility response....
Veronika Gondzik et al.
American journal of physiology. Cell physiology, 303(9), C936-C946 (2012-08-24)
The mammalian collecting duct (CD) is continuously exposed to urinary proteases. The CD expresses an epithelial Na(+) channel (ENaC) that is activated after cleavage by serine proteases. ENaC also exists at the plasma membrane in the uncleaved form, rendering activation...
Gang Yu et al.
Journal of microbiology (Seoul, Korea), 50(6), 939-946 (2013-01-01)
The entomopathogenic fungus Verticillium lecanii is a well-known biocontrol agent. V. lecanii produces subtilisin-like serine protease (Pr1), which is important in the biological control activity of some insect pests by degrading insect cuticles. In this study, a subtilisin-like serine protease...
Tadashi Nakai et al.
The Journal of biological chemistry, 287(9), 6530-6538 (2012-01-12)
Quinohemoprotein amine dehydrogenase (QHNDH), an αβγ heterotrimer present in the periplasm of several Gram-negative bacteria, catalyzes the oxidative deamination of various aliphatic amines such as n-butylamine for assimilation as carbon and energy sources. The γ subunit of mature QHNDH contains...
Qing Zhu et al.
Applied and environmental microbiology, 73(19), 6053-6057 (2007-08-07)
Nitrilases have found wide use in the pharmaceutical industry for the production of fine chemicals, and it is important to have a method by which to screen libraries of isolated or engineered nitrilase variants (including bacteria and fungi). The conventional...
Li-Yuan Ran et al.
The Journal of biological chemistry, 289(9), 6041-6053 (2014-01-17)
Collagen is an insoluble protein that widely distributes in the extracellular matrix of marine animals. Collagen degradation is an important step in the marine nitrogen cycle. However, the mechanism of marine collagen degradation is still largely unknown. Here, a novel...
Comment on Enhancement of the catalytic activity of a 27 kDa subtilisin-like enzyme from Bacillus amyloliquefaciens CH51 by in vitro mutagenesis.
Beenish Viqar et al.
Journal of agricultural and food chemistry, 60(16), 4170-4172 (2012-04-06)
Oi Wah Liew et al.
Journal of immunological methods, 403(1-2), 37-51 (2013-12-03)
This is a first report of recombinant production of human prepro-Urocortin 2 in Escherichia coli by N-terminal fusion with a triple His₆-SUMO-eXact tag and its subsequent use as an antigen for the production and screening of very high affinity monoclonal...
H Ikemura et al.
The Journal of biological chemistry, 262(16), 7859-7864 (1987-06-05)
Subtilisin E, an alkaline serine protease of Bacillus subtilis 168, is first produced as a precursor, pre-pro-subtilisin, which consists of a signal peptide for protein secretion (pre-sequence) and a peptide extension of 77 amino acid residues (pro-sequence) between the signal...
Jun Yuan et al.
BMC biotechnology, 12, 36-36 (2012-07-04)
Today, thrombosis is one of the most widely occurring diseases in modern life. Drugs with thrombolytic functions are the most effective methods in the treatment of thrombosis. Among them, Douchi fibrinolytic enzyme (DFE) is a promising agent. DFE was isolated...
Derong Lin et al.
Journal of food science and technology, 57(11), 4247-4256 (2020-10-20)
The present study was conducted to evaluate the anti-hyperlipidemia ability of the dietary fiber extracted from okara in mice fed a high cholesterol diet. The dietary fiber was extracted from okara by combining fermentation with dynamic high-pressure microfluidization. An animal...
Diana Lousa et al.
The journal of physical chemistry. B, 116(20), 5838-5848 (2012-05-02)
A recent X-ray structure has enabled the location of chloride and cesium ions on the surface of subtilisin Carlsberg in acetonitrile soaked crystals. (1) To complement the previous study and analyze the system in solution, molecular dynamics (MD) simulations, in...
Dalibor Kodrík et al.
Applied biochemistry and biotechnology, 171(1), 209-224 (2013-07-05)
Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were...
Natthiya Wetsaphan et al.
Developmental and comparative immunology, 41(2), 110-119 (2013-05-11)
Serpin or serine proteinase inhibitor is a family of protease inhibitors that are involved in controlling the proteolytic cascade in various biological processes. In shrimp, several serpins have been identified but only a few have been characterized. Herein, the PmSERPIN3...
Páraic O Cuív et al.
Applied and environmental microbiology, 79(19), 6173-6175 (2013-07-23)
Clostridium thermocellum encodes a cellulosomal, modular, and thermostable serine protease inhibitor (serpin), PinA. PinA stability but not inhibitory activity is affected by the Fn(III) and Doc(I) domains, and PinA is a broad inhibitor of subtilisin-like proteases and may play a...
Yong Zhuo et al.
Journal of animal science and biotechnology, 11, 47-47 (2020-05-20)
This study aimed to investigate the impacts of guar gum and cellulose as the source of dietary fiber during gestation on the reproductive performance of sows. A total of 210 sows (parities 3-6) were randomly allocated into six diets (n = 35)...
Chae Eun Lee et al.
Food science and biotechnology, 29(12), 1695-1703 (2020-12-08)
To increase resistant starch (RS) content of rice flour, soft and normal rice flours were cross-linked with different flour concentrations (40, 50%). RS contents, morphology, and baking qualities of cross-linked Singil rice flour (CSRF) and Hopyeong rice flour (CHRF) were...
Viduthalai Rasheedkhan Regina et al.
ACS applied materials & interfaces, 4(11), 5915-5921 (2012-10-02)
Enzymes with antifouling properties are of great interest in developing nontoxic antifouling coatings. A bottleneck in developing enzyme-based antifouling coatings is to immobilize the enzyme in a suitable coating matrix without compromising its activity and stability. Entrapment of enzymes in...
Laura Domínguez Díaz et al.
Nutrients, 12(5) (2020-05-18)
In Europe, nutrition and health claims made on food must be based on scientific evidence, which means a comprehensive evaluation by the European Food Safety Authority (EFSA) prior to authorisation. Processed foods are subject to numerous claims derived from the...
Youské Shimizu et al.
The FEBS journal, 280(7), 1681-1692 (2013-02-13)
Members of the kinesin-13 sub-family, including KIF2C, depolymerize microtubules. The positive charge-rich 'neck' region extending from the N-terminus of the catalytic head is considered to be important in the depolymerization activity. Chemically synthesized peptides, covering the basic region (A182-E200), induced...
María Belén Fernández et al.
Plant physiology and biochemistry : PPB, 61, 197-203 (2012-11-07)
Programmed cell death (PCD) occurs in plants, animals and several branches of unicellular eukaryotes as a part of developmental and/or defense processes. Caspase proteases are universal mediators of animal apoptosis, a type of PCD. In plants, there are not animal...
Li Huang et al.
Protein expression and purification, 82(1), 55-60 (2011-12-03)
Desensitization of agonist-activated G protein-coupled receptors (GPCRs) requires phosphorylation followed by the binding of arrestin, a ~48 kDa soluble protein. While crystal structures for the inactive, 'basal' state of various arrestins are available, the conformation of 'activated' arrestin adopted upon...
Renxian Wang et al.
Phytochemistry, 95, 118-126 (2013-08-06)
Senescence-related proteases play important roles in leaf senescence by regulating protein degradation and nutrient recycling. A 98.9kDa senescence-related protease EP3 in wheat leaves was purified by ammonium sulfate precipitation, Q-Sepharose fast flow anion exchange chromatography and gel slicing after gel...
Bastien Mangeat et al.
The Journal of biological chemistry, 287(26), 22015-22029 (2012-04-12)
Influenza virus infections lead to a burst of type I interferon (IFN) in the human respiratory tract, which most probably accounts for a rapid control of the virus. Although in mice, IFN-induced Mx1 factor mediates a major part of this...
Savvas Saouros et al.
The Journal of biological chemistry, 287(43), 36029-36040 (2012-08-17)
Toxoplasma gondii is the model parasite of the phylum Apicomplexa, which contains obligate intracellular parasites of medical and veterinary importance. Apicomplexans invade host cells by a multistep process involving the secretion of adhesive microneme protein (MIC) complexes. The subtilisin protease...
Teodolinda Petrillo et al.
Biochemistry, 51(31), 6164-6170 (2012-07-05)
Two new inhibitors in which the terminal α-carboxyl groups of Z-Ala-Ala-Phe-COOH and Z-Ala-Pro-Phe-COOH have been replaced with a proton to give Z-Ala-Ala-Phe-H and Z-Ala-Pro-Phe-H, respectively, have been synthesized. Using these inhibitors, we estimate that for α-chymotrypsin and subtilisin Carlsberg the...
Kusum Solanki et al.
Bioresource technology, 115, 147-151 (2012-01-18)
A first study of the comparison of structures of enzymes (by FT-IR and CD) in different high activity (in low water media) preparations is reported. Using chymotrypsin and subtilisin as models, we have studied various factors that distinguish enzyme precipitated...
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