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Páraic O Cuív et al.
Applied and environmental microbiology, 79(19), 6173-6175 (2013-07-23)
Clostridium thermocellum encodes a cellulosomal, modular, and thermostable serine protease inhibitor (serpin), PinA. PinA stability but not inhibitory activity is affected by the Fn(III) and Doc(I) domains, and PinA is a broad inhibitor of subtilisin-like proteases and may play a...
Proteases: multifunctional enzymes in life and disease
Lopez-Otin C and Bond JS
Test, 283(45), 30433-30437 (2008)
Ghanendra Gartaula et al.
Carbohydrate polymers, 196, 199-208 (2018-06-13)
The cell walls of cereal endosperms are a major source of fibre in many diets and of importance in seed structure and germination. Cell walls were isolated from both pure wheat endosperm and milled flour. 13C CP/MAS NMR in conjunction...
Keiichi Yokoyama et al.
Applied microbiology and biotechnology, 87(6), 2087-2096 (2010-06-04)
Microbial transglutaminase (MTG) has been used extensively in academic research and the food industries through its cross-linking or posttranslational modification of proteins. Two enzyme engineering approaches were applied to improve MTG activity. One is a novel method of rational mutagenesis...
Diana Lousa et al.
The journal of physical chemistry. B, 116(20), 5838-5848 (2012-05-02)
A recent X-ray structure has enabled the location of chloride and cesium ions on the surface of subtilisin Carlsberg in acetonitrile soaked crystals. (1) To complement the previous study and analyze the system in solution, molecular dynamics (MD) simulations, in...
Natthiya Wetsaphan et al.
Developmental and comparative immunology, 41(2), 110-119 (2013-05-11)
Serpin or serine proteinase inhibitor is a family of protease inhibitors that are involved in controlling the proteolytic cascade in various biological processes. In shrimp, several serpins have been identified but only a few have been characterized. Herein, the PmSERPIN3...
Dalibor Kodrík et al.
Applied biochemistry and biotechnology, 171(1), 209-224 (2013-07-05)
Several recombinant derivatives of serine protease inhibitor called silk protease inhibitor 2 (SPI2), which is a silk component in Galleria mellonella (Lepidoptera, Insecta), were prepared in the expression vector Pichia pastoris. Both the native and the recombinant protease inhibitors were...
[The use of single-dose total body irradiation before bone marrow transplantation in treatment of leukemia].
H Tang
Zhonghua fang she xue za zhi Chinese journal of radiology, 16(4), 296-300 (1982-11-01)
Vivek Kumar Morya et al.
Applied biochemistry and biotechnology, 166(1), 243-257 (2011-11-11)
A total of 49 protein sequences of alkaline proteases retrieved from GenBank representing different species of Aspergillus have been characterized for various physiochemical properties, homology search, multiple sequence alignment, motif, and super family search and phylogenetic tree construction. The sequence...
Gang Yu et al.
Journal of microbiology (Seoul, Korea), 50(6), 939-946 (2013-01-01)
The entomopathogenic fungus Verticillium lecanii is a well-known biocontrol agent. V. lecanii produces subtilisin-like serine protease (Pr1), which is important in the biological control activity of some insect pests by degrading insect cuticles. In this study, a subtilisin-like serine protease...
Catherine Cesa-Luna et al.
PloS one, 15(10), e0240545-e0240545 (2020-10-16)
The bacterial strain, EMM-1, was isolated from the rhizosphere of red maize ("Rojo Criollo") and identified as Pseudomonas protegens EMM-1 based on phylogenetic analysis of 16S rDNA, rpoB, rpoD, and gyrB gene sequences. We uncovered genes involved in the production...
Tadashi Nakai et al.
The Journal of biological chemistry, 287(9), 6530-6538 (2012-01-12)
Quinohemoprotein amine dehydrogenase (QHNDH), an αβγ heterotrimer present in the periplasm of several Gram-negative bacteria, catalyzes the oxidative deamination of various aliphatic amines such as n-butylamine for assimilation as carbon and energy sources. The γ subunit of mature QHNDH contains...
S M Krieger et al.
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association, 59, 165-176 (2013-06-12)
There remains a need for a simple and predictive animal model to identify potential respiratory sensitizers. The mouse intranasal test (MINT) was developed to assess the relative allergic potential of detergent enzymes, however, the experimental endpoints were limited to evaluation...
Jun Yuan et al.
BMC biotechnology, 12, 36-36 (2012-07-04)
Today, thrombosis is one of the most widely occurring diseases in modern life. Drugs with thrombolytic functions are the most effective methods in the treatment of thrombosis. Among them, Douchi fibrinolytic enzyme (DFE) is a promising agent. DFE was isolated...
Oi Wah Liew et al.
Journal of immunological methods, 403(1-2), 37-51 (2013-12-03)
This is a first report of recombinant production of human prepro-Urocortin 2 in Escherichia coli by N-terminal fusion with a triple His₆-SUMO-eXact tag and its subsequent use as an antigen for the production and screening of very high affinity monoclonal...
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Li-Yuan Ran et al.
The Journal of biological chemistry, 289(9), 6041-6053 (2014-01-17)
Collagen is an insoluble protein that widely distributes in the extracellular matrix of marine animals. Collagen degradation is an important step in the marine nitrogen cycle. However, the mechanism of marine collagen degradation is still largely unknown. Here, a novel...
Karina Jiménez-Durán et al.
Plant physiology, 161(1), 97-107 (2012-11-15)
In Solanaceae, the self-incompatibility S-RNase and S-locus F-box interactions define self-pollen recognition and rejection in an S-specific manner. This interaction triggers a cascade of events involving other gene products unlinked to the S-locus that are crucial to the self-incompatibility response....
Xiaoqin Wang et al.
Biotechnology progress, 29(3), 681-687 (2013-04-23)
Chlorella pyrenoidosa is a unicellular green algae and has been a popular foodstuff worldwide. However, no reports on the antitumor peptides from such a microalgae are available in the literature. In this study, using low-temperature high-pressure extraction, enzymatic hydrolysis, ion...
Tugba Kilic et al.
Biofouling, 33(4), 352-367 (2017-04-21)
The ability of Aeribacillus pallidus E334 to produce pellicle and form a biofilm was studied. Optimal biofilm formation occurred at 60 °C, pH 7.5 and 1.5% NaCl. Extra polymeric substances (EPS) were composed of proteins and eDNA (21.4 kb). E334 formed...
Comment on Enhancement of the catalytic activity of a 27 kDa subtilisin-like enzyme from Bacillus amyloliquefaciens CH51 by in vitro mutagenesis.
Beenish Viqar et al.
Journal of agricultural and food chemistry, 60(16), 4170-4172 (2012-04-06)
Veronika Gondzik et al.
American journal of physiology. Cell physiology, 303(9), C936-C946 (2012-08-24)
The mammalian collecting duct (CD) is continuously exposed to urinary proteases. The CD expresses an epithelial Na(+) channel (ENaC) that is activated after cleavage by serine proteases. ENaC also exists at the plasma membrane in the uncleaved form, rendering activation...
Fu-Yuan Cheng et al.
Animal science journal = Nihon chikusan Gakkaiho, 80(1), 91-97 (2010-02-19)
This study aims to identify peptides with angiotensin-I converting enzyme (ACE) inhibitory activity in hydrolysate from chicken leg bone protein hydrolyzed with alcalase for 4 h (A4H). The hydrolysate has demonstrated potent in vitro ACE inhibitory activity, and has been...
Juan M Martínez-Brown et al.
Journal of morphology, 280(7), 948-967 (2019-04-26)
The embryonic development of the bullseye puffer, Sphoeroides annulatus, was characterized on the basis of the theory of saltatory ontogeny. This theory predicts a correlative relationship between the ontogeny-type in an altricial-precocial spectrum and the habitat that a species occupies...
H Ikemura et al.
The Journal of biological chemistry, 262(16), 7859-7864 (1987-06-05)
Subtilisin E, an alkaline serine protease of Bacillus subtilis 168, is first produced as a precursor, pre-pro-subtilisin, which consists of a signal peptide for protein secretion (pre-sequence) and a peptide extension of 77 amino acid residues (pro-sequence) between the signal...
Bo Wang et al.
Food chemistry, 245, 646-652 (2017-12-31)
Antioxidant casein peptides were separated by SP-Sephadex C-25 and C 18 columns. The transepithelial transport and bioavailability including the transport ratio and the remaining ratios of antioxidant activity (RRAA) of these peptide absorbates, were then investigated using a Caco-2 cell...
Renxian Wang et al.
Phytochemistry, 95, 118-126 (2013-08-06)
Senescence-related proteases play important roles in leaf senescence by regulating protein degradation and nutrient recycling. A 98.9kDa senescence-related protease EP3 in wheat leaves was purified by ammonium sulfate precipitation, Q-Sepharose fast flow anion exchange chromatography and gel slicing after gel...
Savvas Saouros et al.
The Journal of biological chemistry, 287(43), 36029-36040 (2012-08-17)
Toxoplasma gondii is the model parasite of the phylum Apicomplexa, which contains obligate intracellular parasites of medical and veterinary importance. Apicomplexans invade host cells by a multistep process involving the secretion of adhesive microneme protein (MIC) complexes. The subtilisin protease...
Daixi Liu et al.
Gene, 514(2), 99-104 (2012-12-04)
A 255-bp cDNA encoding an 84-amino acid residue (aa) precursor protein containing 8 half-cysteines was cloned from the skin of the frog, Ceratophrys calcarata. By sequence comparison and signal peptide prediction, the precursor was predicted to release a 63-aa mature...
Younes Ghasemi et al.
Molecular biotechnology, 52(1), 1-7 (2011-11-10)
Several investigations are being pursued to enhance the efficacy and specificity of fibrinolytic therapy. In this regard, microbial fibrinolytic enzymes attracted much more medical interests during these decades. Subtilisin, a member of subtilases (the superfamily of subtilisin-like serine proteases) and...
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