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Alberto Barbiroli et al.
Biomolecules, 9(10) (2019-10-17)
The search for bioactivities influencing the human wellbeing of food proteins and peptides is a topic of broad and current interest. γ-Conglutin (γC) is a lupin seed protein drawing remarkable pharmacological and/or nutraceutical interest, as it is able to reduce...
Yi Yang et al.
Food chemistry, 331, 127315-127315 (2020-06-28)
The effects of frying temperature on the properties of wheat starch were investigated, including the microstructure, weight-average molar mass (Mw), crystalline properties, functional groups, in vitro digestibility, static rheological properties, swelling power and solubility. SEM micrographs showed that the disc-shaped...
Enzymes of Molecular Biology
Sweeney, P.J., and Walker, J.M.
Methods in Molecular Biology, 290-291 (1993)
Mahmoud S Ali et al.
The Laryngoscope, 123(2), 434-439 (2012-10-17)
To assess if, as previously reported in the literature, bile acids inhibit pepsin activity, resulting in pepsin having a less important role in laryngopharyngeal damage in reflux disease. Prospective translational research study. A total of 78 patient's fasting gastric juice...
Benchawan Jityuti et al.
Journal of photochemistry and photobiology. B, Biology, 126, 55-59 (2013-07-31)
In this study, a molybdenum(VI) peroxo α-amino acid complex, MoO(O2)2(α-leucine) (H2O), was prepared and used as an artificial protease for site-specific cleavage of porcine pepsin, a model protein. Cleavage of pepsin by MoO(O2)2(α-leucine) (H2O) was achieved under photochemical conditions at...
Lucie Lorieau et al.
Food chemistry, 245, 454-462 (2017-12-31)
Numerous calcium sources are available to enrich food, but their behavior during digestion is still unknown. This study focused on the influence of the gastro-intestinal pH, the food structure and the calcium source on the bioaccessibility of the nutrient. Four...
Birthe Dorgau et al.
Biomaterials, 199, 63-75 (2019-02-10)
Tissue specific extracellular matrices (ECM) provide structural support and enable access to molecular signals and metabolites, which are essential for directing stem cell renewal and differentiation. To mimic this phenomenon in vitro, tissue decellularisation approaches have been developed, resulting in...
Ning Chen et al.
Peptides, 38(2), 344-349 (2012-10-02)
Walnut proteins were hydrolyzed separately using three different proteases to obtain antioxidant peptides. The antioxidant activities of the hydrolysates were measured using 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay. Among hydrolysates, pepsin hydrolysate obtained by 3h exhibited the highest antioxidant activities, which could...
Sebastiano Di Bucchianico et al.
Journal of nanobiotechnology, 9, 27-27 (2011-06-29)
Chromosomal dissection provides a direct advance for isolating DNA from cytogenetically recognizable region to generate genetic probes for fluorescence in situ hybridization, a technique that became very common in cyto and molecular genetics research and diagnostics. Several reports describing microdissection...
Ying Zou et al.
Molecular and cellular biology, 29(9), 2390-2397 (2009-02-19)
The molecular distinctions between mortality stages 1 (M1; senescence) and 2 (M2; crisis) of human replicative aging are ill defined. We demonstrate a qualitative difference between telomeric end associations at M1 and the end fusions that produce dicentric chromosomes and...
D Lee et al.
The Biochemical journal, 104(3), 735-741 (1967-09-01)
Methods are described for the isolation and purification of pepsinogen D, a minor zymogen occurring to the extent of about 5% of the potential proteolytic activity in neutral extracts of the pig gastric mucosa. The physical and chemical properties of...
Thomas J Broekelmann et al.
Matrix biology : journal of the International Society for Matrix Biology, 27(7), 631-639 (2008-07-08)
The carboxy-terminus of tropoelastin is a highly conserved, atypical region of the molecule with sequences that define both cell and matrix interactions. This domain also plays a critical but unknown role in the assembly and crosslinking of tropoelastin during elastic...
Edoardo Bertini et al.
Frontiers in plant science, 9, 572-572 (2018-05-17)
Type-1 diabetes (T1D) is a metabolic disease involving the autoimmune destruction of insulin-producing pancreatic beta cells. It is often diagnosed by the detection of autoantibodies, typically those recognizing insulin itself or the 65-kDa isoform of glutamic acid decarboxylase (GAD65). Oral...
Gabriel Dasilva et al.
The Journal of nutritional biochemistry, 55, 68-75 (2018-02-08)
Previous research showed that increasing the proportion of docosahexaenoic acid (DHA) in marine lipid supplements significantly reduces associated health benefits compared with balanced eicosapentaenoic acid (EPA):DHA supplementation Dasilva et al., 2015 [1]. It was therefore hypothesized that the EPA and...
Gilda Aiello et al.
Foods (Basel, Switzerland), 9(8) (2020-08-14)
The supplementation of different food items with grain legumes and, in particular, with lupin has been demonstrated to provide useful health benefits, especially in the area of cardiovascular disease prevention. In this work, label free quantitative untargeted and targeted approaches...
Zixuan Gu et al.
Food chemistry, 343, 128485-128485 (2020-11-12)
The aim of this study was to prepare a supporting carrier, namely highly branched corn starch (HBCS), and to investigate its encapsulation property with ascorbic acid (AA). High amylose corn starch was converted into HBCS via dual enzymatic modification by...
Doron Boltin et al.
Best practice & research. Clinical gastroenterology, 28(6), 981-994 (2014-12-03)
The gastric barrier contains several lines of defence which protect the epithelium from harmful microbes and toxins. Pre-mucosal defence mechanisms include secreted acid (HCl 0.1 mmol/L) and pepsin, which are capable of denaturing tissue. A tightly adherent mucous layer provides...
T I Chelpanova et al.
Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova, 98(6), 734-743 (2012-09-28)
Pectin substances were extracted from food plants: sweet pepper Capsicum annuum L., carrot sowing Daucus sativus L., bulb onion Allium cepa L., white cabbage Brassica oleracea L. by two methods with acid solutions similar to gastric environment. The pectins that...
Ana-Isabel Mulet-Cabero et al.
Food chemistry, 319, 126514-126514 (2020-03-18)
Caseins and whey proteins are known as 'slow' and 'fast' proteins, respectively, based on their amino acid absorption rate. However, there is limited understanding of the mechanisms controlling their behaviour during gastro-intestinal transit. A protein model system (8% total protein)...
Jane N Eriksen et al.
Food chemistry, 224, 407-413 (2017-02-06)
Dark green leafy vegetables are primary food sources for lutein and β-carotene, however these bioactives have low bioavailability. The effects of mechanical and thermal processing as well as fat addition and fat type on lutein and β-carotene liberation and in...
Gabriele Rocchetti et al.
Foods (Basel, Switzerland), 9(5) (2020-05-20)
Fresh pasta was formulated by replacing wheat semolina with 0, 5, 10, and 15 g/100 g (w/w) of Moringa oleifera L. leaf powder (MOLP). The samples (i.e., M0, M5, M10, and M15 as a function of the substitution level) were...
Sunbin Kim et al.
Food chemistry, 343, 128437-128437 (2020-11-10)
The effective delivery of bioactive compounds has recently been receiving attention. In this study, a conjugate with BSA and fucoidan synthesized via the Maillard reaction was confirmed through electrophoresis, the o-phthalaldehyde assay, and through changes in absorbance. Two moles of...
J Tang et al.
Journal of cellular biochemistry, 33(1), 53-63 (1987-01-01)
Aspartic proteases (EC3.4.23) are a group of proteolytic enzymes of the pepsin family that share the same catalytic apparatus and usually function in acid solutions. This latter aspect limits the function of aspartic proteases to some specific locations in different...
Yang Wei et al.
Food & function, 11(11), 9973-9983 (2020-10-30)
The novel zein-propylene glycol alginate (PGA) -tea saponin (TS) ternary complex nanoparticles were fabricated to deliver resveratrol. TS was firstly introduced to modulate the functional attributes, microstructure, molecular interactions and gastrointestinal digestion of the complex nanoparticles. The size of zein-PGA-TS...
Linda Le Roux et al.
Food research international (Ottawa, Ont.), 130, 108917-108917 (2020-03-12)
Infant formulas (IFs) are the key nutritional source for infants who cannot be breastfed. There is currently a growing interest in these sensitive products in order to control their quality and to design their composition with regard to nutritional balance....
Shannon Gwala et al.
Food research international (Ottawa, Ont.), 137, 109512-109512 (2020-11-26)
When pulse seeds from a single batch are cooked, considerable variability of hardness values in the population is usually observed. Sorting the seeds into hardness categories could reduce the observed diversity and increase uniformity. Therefore, we investigated the effect of...
Ana-Isabel Mulet-Cabero et al.
Food & function, 11(2), 1702-1720 (2020-02-11)
The link between food and human health is increasingly a topic of interest. One avenue of study has been to assess food disintegration and interactions within the gastrointestinal tract. In vitro digestion models have been widely used to overcome the...
Olivia Ogilvie et al.
MethodsX, 7, 101076-101076 (2020-10-10)
Mass spectrometry (MS) is an emerging method to determine the accurate concentration of immunogenic gluten peptides. It is of interest to quantify specific peptides within the gluten peptidome due to the role they play in the activation of the celiac...
Linda Le Roux et al.
Foods (Basel, Switzerland), 9(3) (2020-04-05)
Infant formulas (IFs) are used as substitutes for human milk and are mostly based on cow milk proteins. For sustainability reasons, animal protein alternatives in food are increasingly being considered, as plant proteins offer interesting nutritional and functional benefits for...
O Molberg et al.
Nature medicine, 4(6), 713-717 (1998-06-12)
The action of tissue Transglutaminase (TGase) on specific protein-bound glutamine residues plays a critical role in numerous biological processes. Here we provide evidence for a new role of this enzyme in the common, HLA-DQ2 (and DQ8) associated enteropathy, celiac disease...
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