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Thomas J Broekelmann et al.
Matrix biology : journal of the International Society for Matrix Biology, 27(7), 631-639 (2008-07-08)
The carboxy-terminus of tropoelastin is a highly conserved, atypical region of the molecule with sequences that define both cell and matrix interactions. This domain also plays a critical but unknown role in the assembly and crosslinking of tropoelastin during elastic
Mahmoud S Ali et al.
The Laryngoscope, 123(2), 434-439 (2012-10-17)
To assess if, as previously reported in the literature, bile acids inhibit pepsin activity, resulting in pepsin having a less important role in laryngopharyngeal damage in reflux disease. Prospective translational research study. A total of 78 patient's fasting gastric juice
Yi Yang et al.
Food chemistry, 331, 127315-127315 (2020-06-28)
The effects of frying temperature on the properties of wheat starch were investigated, including the microstructure, weight-average molar mass (Mw), crystalline properties, functional groups, in vitro digestibility, static rheological properties, swelling power and solubility. SEM micrographs showed that the disc-shaped
Enzymes of Molecular Biology
Sweeney, P.J., and Walker, J.M.
Methods in Molecular Biology, 290-291 (1993)
Benchawan Jityuti et al.
Journal of photochemistry and photobiology. B, Biology, 126, 55-59 (2013-07-31)
In this study, a molybdenum(VI) peroxo α-amino acid complex, MoO(O2)2(α-leucine) (H2O), was prepared and used as an artificial protease for site-specific cleavage of porcine pepsin, a model protein. Cleavage of pepsin by MoO(O2)2(α-leucine) (H2O) was achieved under photochemical conditions at
Alberto Barbiroli et al.
Biomolecules, 9(10) (2019-10-17)
The search for bioactivities influencing the human wellbeing of food proteins and peptides is a topic of broad and current interest. γ-Conglutin (γC) is a lupin seed protein drawing remarkable pharmacological and/or nutraceutical interest, as it is able to reduce
Gabriele Rocchetti et al.
Foods (Basel, Switzerland), 9(5) (2020-05-20)
Fresh pasta was formulated by replacing wheat semolina with 0, 5, 10, and 15 g/100 g (w/w) of Moringa oleifera L. leaf powder (MOLP). The samples (i.e., M0, M5, M10, and M15 as a function of the substitution level) were
Lucie Lorieau et al.
Food chemistry, 245, 454-462 (2017-12-31)
Numerous calcium sources are available to enrich food, but their behavior during digestion is still unknown. This study focused on the influence of the gastro-intestinal pH, the food structure and the calcium source on the bioaccessibility of the nutrient. Four
Ying Zou et al.
Molecular and cellular biology, 29(9), 2390-2397 (2009-02-19)
The molecular distinctions between mortality stages 1 (M1; senescence) and 2 (M2; crisis) of human replicative aging are ill defined. We demonstrate a qualitative difference between telomeric end associations at M1 and the end fusions that produce dicentric chromosomes and
Sebastiano Di Bucchianico et al.
Journal of nanobiotechnology, 9, 27-27 (2011-06-29)
Chromosomal dissection provides a direct advance for isolating DNA from cytogenetically recognizable region to generate genetic probes for fluorescence in situ hybridization, a technique that became very common in cyto and molecular genetics research and diagnostics. Several reports describing microdissection
Ning Chen et al.
Peptides, 38(2), 344-349 (2012-10-02)
Walnut proteins were hydrolyzed separately using three different proteases to obtain antioxidant peptides. The antioxidant activities of the hydrolysates were measured using 1,1-diphenyl-2-picryl hydrazyl (DPPH) assay. Among hydrolysates, pepsin hydrolysate obtained by 3h exhibited the highest antioxidant activities, which could
Sunbin Kim et al.
Food chemistry, 343, 128437-128437 (2020-11-10)
The effective delivery of bioactive compounds has recently been receiving attention. In this study, a conjugate with BSA and fucoidan synthesized via the Maillard reaction was confirmed through electrophoresis, the o-phthalaldehyde assay, and through changes in absorbance. Two moles of
Edoardo Bertini et al.
Frontiers in plant science, 9, 572-572 (2018-05-17)
Type-1 diabetes (T1D) is a metabolic disease involving the autoimmune destruction of insulin-producing pancreatic beta cells. It is often diagnosed by the detection of autoantibodies, typically those recognizing insulin itself or the 65-kDa isoform of glutamic acid decarboxylase (GAD65). Oral
Birthe Dorgau et al.
Biomaterials, 199, 63-75 (2019-02-10)
Tissue specific extracellular matrices (ECM) provide structural support and enable access to molecular signals and metabolites, which are essential for directing stem cell renewal and differentiation. To mimic this phenomenon in vitro, tissue decellularisation approaches have been developed, resulting in
Ana-Isabel Mulet-Cabero et al.
Food chemistry, 319, 126514-126514 (2020-03-18)
Caseins and whey proteins are known as 'slow' and 'fast' proteins, respectively, based on their amino acid absorption rate. However, there is limited understanding of the mechanisms controlling their behaviour during gastro-intestinal transit. A protein model system (8% total protein)
Andree Schmidt et al.
Journal of leukocyte biology, 81(1), 186-194 (2006-10-21)
Alveolar macrophages (AMs) and peribronchial/perivascular macrophages are probably involved in lung allograft damage. We investigate leukocyte infiltration into graft tissue and address the question whether proliferation in situ contributes to macrophage homeostasis and accumulation. Lung transplantation was performed in the
T I Chelpanova et al.
Rossiiskii fiziologicheskii zhurnal imeni I.M. Sechenova, 98(6), 734-743 (2012-09-28)
Pectin substances were extracted from food plants: sweet pepper Capsicum annuum L., carrot sowing Daucus sativus L., bulb onion Allium cepa L., white cabbage Brassica oleracea L. by two methods with acid solutions similar to gastric environment. The pectins that
Gilda Aiello et al.
Foods (Basel, Switzerland), 9(8) (2020-08-14)
The supplementation of different food items with grain legumes and, in particular, with lupin has been demonstrated to provide useful health benefits, especially in the area of cardiovascular disease prevention. In this work, label free quantitative untargeted and targeted approaches
Gabriel Dasilva et al.
The Journal of nutritional biochemistry, 55, 68-75 (2018-02-08)
Previous research showed that increasing the proportion of docosahexaenoic acid (DHA) in marine lipid supplements significantly reduces associated health benefits compared with balanced eicosapentaenoic acid (EPA):DHA supplementation Dasilva et al., 2015 [1]. It was therefore hypothesized that the EPA and
Mariantonietta Succi et al.
Frontiers in microbiology, 8, 1067-1067 (2017-07-01)
In our study, we dwelled upon combinations of lactobacilli/prebiotics, considering four different strains belonging to the
Zixuan Gu et al.
Food chemistry, 343, 128485-128485 (2020-11-12)
The aim of this study was to prepare a supporting carrier, namely highly branched corn starch (HBCS), and to investigate its encapsulation property with ascorbic acid (AA). High amylose corn starch was converted into HBCS via dual enzymatic modification by
Linda Le Roux et al.
Foods (Basel, Switzerland), 9(3) (2020-04-05)
Infant formulas (IFs) are used as substitutes for human milk and are mostly based on cow milk proteins. For sustainability reasons, animal protein alternatives in food are increasingly being considered, as plant proteins offer interesting nutritional and functional benefits for
J Tang et al.
Journal of cellular biochemistry, 33(1), 53-63 (1987-01-01)
Aspartic proteases (EC3.4.23) are a group of proteolytic enzymes of the pepsin family that share the same catalytic apparatus and usually function in acid solutions. This latter aspect limits the function of aspartic proteases to some specific locations in different
D Lee et al.
The Biochemical journal, 104(3), 735-741 (1967-09-01)
Methods are described for the isolation and purification of pepsinogen D, a minor zymogen occurring to the extent of about 5% of the potential proteolytic activity in neutral extracts of the pig gastric mucosa. The physical and chemical properties of
João P B Oliveira et al.
Food chemistry, 255, 260-267 (2018-03-25)
Transitory allergies to cow milk proteins in infants or adults have become a public health problem. Although extensively or partially hydrolyzed cow milk protein formulas are available, these products are costly. Therefore, studies into innovative enzymes to digest cow milk
Junjie Lan et al.
Frontiers in pharmacology, 12, 744409-744409 (2021-11-12)
Ethnopharmacological relevance: Two types of traditional Chinese formulas of botanical drugs are prescribed for treating perimenopausal syndrome (PMS), a disorder in middle-aged women during their transition to menopause. One is for treating PMS as kidney deficiency (KD) due to senescence
M E Arnal et al.
Beneficial microbes, 12(4), 75-90 (2021-06-11)
Health benefits of probiotics in humans essentially depend on their ability to survive during gastrointestinal (GI) transit and to modulate gut microbiota. To date, there is few data on the impact of galenic formulations of probiotics on these parameters. Even
Olivia Ogilvie et al.
MethodsX, 7, 101076-101076 (2020-10-10)
Mass spectrometry (MS) is an emerging method to determine the accurate concentration of immunogenic gluten peptides. It is of interest to quantify specific peptides within the gluten peptidome due to the role they play in the activation of the celiac
Ganqi Deng et al.
Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy, 229, 117935-117935 (2020-01-18)
The objective of this study was to use advanced vibrational molecular spectroscopy (ATR-Ft/IRS) to study an interaction between legume protein molecular structure from biodegradation residues and nutritional properties of newly developed genotypes of cool-climate adapted faba bean seed with low
Bruno J R Gregório et al.
Molecules (Basel, Switzerland), 25(6) (2020-03-19)
This work proposes a simple and easy-to-use flow-through system for the implementation of dynamic extractions, aiming at the evaluation of bioaccessible zinc and the characterization of leaching kinetics in dry dog food samples. The kinetic profile of Zn extraction was
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