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  • Relative levels of dietary EPA and DHA impact gastric oxidation and essential fatty acid uptake.

Relative levels of dietary EPA and DHA impact gastric oxidation and essential fatty acid uptake.

The Journal of nutritional biochemistry (2018-02-08)
Gabriel Dasilva, Matthew Boller, Isabel Medina, Judith Storch
摘要

Previous research showed that increasing the proportion of docosahexaenoic acid (DHA) in marine lipid supplements significantly reduces associated health benefits compared with balanced eicosapentaenoic acid (EPA):DHA supplementation Dasilva et al., 2015 [1]. It was therefore hypothesized that the EPA and DHA molecules might have differential resistance to oxidation during gastric digestion and that the oxidation level achieved could be inversely correlated with intestinal absorption and, hence, with the resultant health benefits. Accordingly, we tested this proposed mechanism of action by investigating the degree of oxidation in the stomach, and the levels of bioaccessible lipids, of varying molar proportions of DHA and EPA (2:1, 1:1 and 1:2) using the dynamic gastrointestinal tract model TIM-1. In addition, small intestine enterocyte absorption and metabolism were simulated by Caco-2 cell monolayers that were incubated with these same varying proportions of DHA and EPA, and comparing oxidized and nonoxidized polyunsaturated fatty acids (PUFAs). The results show an inverse correlation between lipid oxidation products in the stomach and the levels of bioaccessible lipids. The balanced 1:1 EPA:DHA diet resulted in lower oxidation of PUFAs during stomach digestion relative to the other ratios tested. Finally, cell-based studies showed significantly lower assimilation of oxidized EPA and DHA substrates compared to nonoxidized PUFAs, as well as significant differences between the net uptake of EPA and DHA. Overall, the present work suggests that the correct design of diets and/or supplements containing marine lipids can strongly influence the stability and bioaccessibility of PUFAs during gastrointestinal digestion and subsequent absorption. This could modulate their health benefits related with inflammation, oxidative stress and metabolic disorders.

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Sigma-Aldrich
Triton X-100, laboratory grade
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Protease Inhibitor Cocktail, for use with mammalian cell and tissue extracts, DMSO solution
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胃蛋白酶 来源于猪胃粘膜, lyophilized powder, ≥2,500 units/mg protein (E1%/280)
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胰蛋白酶 来源于牛胰腺, powder, ≥7,500 BAEE units/mg solid
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α-淀粉酶 来源于芽孢杆菌 属, Type II-A, lyophilized powder, ≥1,500 units/mg protein (biuret)
牛胆酸钠, BRP, European Pharmacopoeia (EP) Reference Standard