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  • Effect of lipopeptide structure on gene delivery system properties: Evaluation in 2D and 3D in vitro models.

Effect of lipopeptide structure on gene delivery system properties: Evaluation in 2D and 3D in vitro models.

Colloids and surfaces. B, Biointerfaces (2018-04-24)
O O Koloskova, A M Gileva, M G Drozdova, M V Grechihina, N E Suzina, U A Budanova, Yu L Sebyakin, D A Kudlay, I P Shilovskiy, A M Sapozhnikov, E I Kovalenko, E A Markvicheva, M R Khaitov
摘要

Development of efficient biodegradable, environmentally responsive, biocompatible and non-toxic delivery system is needed for efficient gene delivery. As well known, properties of the vehicle are determined by the structure of carrier components. The aim of the current study was to estimate in vitro transfection efficacy of aliphatic di-, tri- and tetrapeptide-based cationic lipoplexes loaded with siRNA in function of a number of cationic groups using 2D (monolayer culture) and 3D (multicellular tumor spheroids) in vitro models. Physicochemical properties and cytotoxicity of the liposomes were found to be dependent upon a number of amino acid derivatives in an amphiphilic polar head. Uptake of liposomes loaded with nucleic acid (lipoplexes) and their localization in HEK293T cells was studied by confocal microscopy. The liposomes based on lipotripeptides had the highest transfection efficiency which was 20-fold higher than those fabricated from lipotetrapeptides.

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Sigma-Aldrich
内消旋 -四苯基卟啉, BioReagent, suitable for fluorescence, ≥99.0% (HPLC)
Sigma-Aldrich
(4-羧丁基)三苯基溴化膦, 98%