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Quantitative structural organisation model for wheat endosperm cell walls: Cellulose as an important constituent.

Carbohydrate polymers (2018-06-13)
Ghanendra Gartaula, Sushil Dhital, Gabriele Netzel, Bernadine M Flanagan, Gleb E Yakubov, Cherie T Beahan, Helen M Collins, Rachel A Burton, Antony Bacic, Michael J Gidley
摘要

The cell walls of cereal endosperms are a major source of fibre in many diets and of importance in seed structure and germination. Cell walls were isolated from both pure wheat endosperm and milled flour. 13C CP/MAS NMR in conjunction with methylation analysis before and after acid hydrolysis showed that, in addition to arabinoxylan (AX) and (1, 3; 1, 4)-β-D-glucan (MLG), wheat endosperm cell walls contain a significant proportion of cellulose (ca 20%) which is tightly bound to xylans and mannans. Light microscopy showed that the cellulose was relatively evenly distributed across the grain endosperm. The cell walls contain a fibrous acid-resistant core structure laminated by matrix polysaccharides as revealed by AFM imaging. A model for endosperm cell wall structural organisation is proposed, based on a core of cellulose and interacting non-cellulosic polysaccharides which anchors AX (with very occasional diferulic acid cross-linking) that in turn retains MLGs through physical entanglement.

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Sigma-Aldrich
蛋白酶 来源于地衣芽孢杆菌, ≥2.4 U/g
Sigma-Aldrich
烯丙基二甲基砜, 96%