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  • Eradication of unresectable liver metastasis through induction of tumour specific energy depletion.

Eradication of unresectable liver metastasis through induction of tumour specific energy depletion.

Nature communications (2019-07-13)
Da Huo, Jianfeng Zhu, Guojun Chen, Qian Chen, Chao Zhang, Xingyu Luo, Wei Jiang, Xiqun Jiang, Zhen Gu, Yong Hu
摘要

Treatment of liver metastasis experiences slow progress owing to the severe side effects. In this study, we demonstrate a strategy capable of eliminating metastatic cancer cells in a selective manner. Nucleus-targeting W18O49 nanoparticles (WONPs) are conjugated to mitochondria-selective mesoporous silica nanoparticles (MSNs) containing photosensitizer (Ce6) through a Cathepsin B-cleavable peptide. In hepatocytes, upon the laser irradiation, the generated singlet oxygen species are consumed by WONPs, in turn leading to the loss of their photothermally heating capacity, thereby sparing hepatocyte from thermal damage induced by the laser illumination. By contrast, in cancer cells, the cleaved peptide linker allows WONPs and MSNs to respectively target nucleus and mitochondria, where the therapeutic powers could be unleashed, both photodynamically and photothermally. This ensures the energy production of cancer cells can be abolished. We further assess the underlying molecular mechanism at both gene and protein levels to better understand the therapeutic outcome.

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内消旋 -四苯基卟啉, BioReagent, suitable for fluorescence, ≥99.0% (HPLC)
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N-Acetyl-Asp-Glu-Val-Asp-al, ≥95%, powder
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