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  • AtHESPERIN: a novel regulator of circadian rhythms with poly(A)-degrading activity in plants.

AtHESPERIN: a novel regulator of circadian rhythms with poly(A)-degrading activity in plants.

RNA biology (2015-12-01)
Costas Delis, Afrodite Krokida, Anastasia Tomatsidou, Daniela Tsikou, Rafailia A A Beta, Maria Tsioumpekou, Julietta Moustaka, Georgios Stravodimos, Demetres D Leonidas, Nikolaos A A Balatsos, Kalliope K Papadopoulou
摘要

We report the identification and characterization of a novel gene, AtHesperin (AtHESP) that codes for a deadenylase in Arabidopsis thaliana. The gene is under circadian clock-gene regulation and has similarity to the mammalian Nocturnin. AtHESP can efficiently degrade poly(A) substrates exhibiting allosteric kinetics. Size exclusion chromatography and native electrophoresis coupled with kinetic analysis support that the native enzyme is oligomeric with at least 3 binding sites. Knockdown and overexpression of AtHESP in plant lines affects the expression and rhythmicity of the clock core oscillator genes TOC1 and CCA1. This study demonstrates an evolutionary conserved poly(A)-degrading activity in plants and suggests deadenylation as a mechanism involved in the regulation of the circadian clock. A role of AtHESP in stress response in plants is also depicted.

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Sigma-Aldrich
poly(A)
Sigma-Aldrich
14,000-500,000分子量非变性蛋白检测试剂盒