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  • Recovery of soluble dietary fiber is dependent on the method of analysis.

Recovery of soluble dietary fiber is dependent on the method of analysis.

The American journal of clinical nutrition (1989-09-01)
J A Marlett, J G Chesters, M J Longacre, J J Bogdanske
摘要

The effects of different methods on the distribution of total neutral sugars (TNS), uronic acids (UA), and beta-glucans (beta G) between the soluble (S) and insoluble (I) fractions of dietary fiber (DF) were determined for peas, kidney beans, oat bran, rice, and macaroni. Incorporation of a protease step into the Theander method "A" modestly increased, and addition of a pepsin digestion further increased the proportion of total fiber recovered in the S fraction. The effect of extraction method on the distribution of TNS, UA, and beta G between the S and I fractions varied with the food. The three methods measured the same total DF in a food and 1-3% starch in the I fraction of peas and kidney beans. Use of dimethyl sulfoxide to solubilize starch, or elevated temperature to extract S components, had no effect on the distribution of DF between S and I fractions of peas and macaroni. Incomplete protein hydrolysis did not always lower Klason lignin and excluding lignin from the fiber complex did not always substantially increase the S fraction.

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Sigma-Aldrich
胃蛋白酶 来源于猪胃粘膜, lyophilized powder, ≥3,200 units/mg protein