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  • The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A; a comparison with morphine-treated cells.

The high-resolution proteomic analysis of protein composition of rat spleen lymphocytes stimulated by Concanavalin A; a comparison with morphine-treated cells.

Journal of neuroimmunology (2020-03-01)
Hana Ujcikova, Kristina Cechova, Lenka Roubalova, Jana Brejchova, Jonas Kaufman, Vladimir Holan, Petr Svoboda
摘要

Morphine- and Concanavalin A-induced changes of protein composition of rat spleen lymphocytes were determined by high-resolution proteomic analysis, gel-free, label-free quantification, MaxLFQ. Stimulation by Con A resulted in a major reorganization of spleen cell protein composition evidenced by increased expression level of 94 proteins; 101 proteins were down-regulated (>2-fold). Interestingly, among proteins that were up-regulated to the largest extent were the prototypical brain proteins as a neuron specific enolase, synapsin-1, brain acid-soluble protein-1 and myelin basic protein. Morphine-induced change was limited to no more than 5 up-regulated and 18 down-regulated proteins (>2-fold).

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Sigma-Aldrich
RPMI-1640 培养基, With L-glutamine and sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture
Sigma-Aldrich
伴刀豆球蛋白A 来源于洋刀豆 (刀豆), biotin conjugate, Type IV, lyophilized powder
Sigma-Aldrich
Prestained Molecular Weight Marker, mol wt 26,600-180,000 Da