Effect of gold nanoparticle aggregation on cell uptake and toxicity.

ACS nano (2011-06-23)
Alexandre Albanese, Warren C W Chan
摘要

Aggregation appears to be a ubiquitous phenomenon among all nanoparticles and its influence in mediating cellular uptake and interactions remain unclear. Here we developed a simple technique to produce transferrin-coated gold nanoparticle aggregates of different sizes and characterized their uptake and toxicity in three different cell lines. While the aggregation did not elicit a unique toxic response, the uptake patterns were different between single and aggregated nanoparticles. There was a 25% decrease in uptake of aggregated nanoparticles with HeLa and A549 cells in comparison to single and monodisperse nanoparticles. However, there was a 2-fold increase in MDA-MB 435 cell uptake for the largest synthesized aggregates. These contrasting results suggest that cell type and the mechanism of interactions may play a significant role. This study highlights the need to investigate the behavior of aggregates with cells on a case-by-case basis and the importance of aggregation in mediating targeting and intracellular trafficking.

材料
货号
品牌
产品描述

Sigma-Aldrich
Gold nanoparticles, 20 nm diameter, amine functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 40 nm diameter, carboxylic acid functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 10 nm diameter, amine functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 40 nm diameter, amine functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 15 nm diameter, amine functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 10 nm diameter, carboxylic acid functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 50 nm diameter, amine functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 50 nm diameter, carboxylic acid functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 5 nm diameter, amine functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 50 nm diameter, amine functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 50 nm diameter, carboxylic acid functionalized, PEG 5000 coated, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 10 nm diameter, amine functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 5 nm diameter, carboxylic acid functionalized, PEG 5000 coated, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 20 nm diameter, carboxylic acid functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 30 nm diameter, amine functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 40 nm diameter, carboxylic acid functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 40 nm diameter, amine functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 30 nm diameter, amine functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 5 nm diameter, amine functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 20 nm diameter, amine functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 20 nm diameter, carboxylic acid functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 5 nm diameter, carboxylic acid functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 15 nm diameter, amine functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 15 nm diameter, carboxylic acid functionalized, PEG 5000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 30 nm diameter, carboxylic acid functionalized, PEG 5000, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 30 nm diameter, carboxylic acid functionalized, PEG 3000 coated, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 10 nm diameter, carboxylic acid functionalized, PEG 3000, OD 50, dispersion in H2O
Sigma-Aldrich
Gold nanoparticles, 15 nm diameter, carboxylic acid functionalized, PEG 3000 coated, OD 50, dispersion in H2O