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The Lipid Handling Capacity of Subcutaneous Fat Is Programmed by mTORC2 during Development.

Cell reports (2020-10-08)
Wen-Yu Hsiao, Su Myung Jung, Yuefeng Tang, John A Haley, Rui Li, Huawei Li, Camila Martinez Calejman, Joan Sanchez-Gurmaches, Chien-Min Hung, Amelia K Luciano, Victoria DeMambro, Kathryn E Wellen, Clifford J Rosen, Lihua Julie Zhu, David A Guertin

Overweight and obesity are associated with type 2 diabetes, non-alcoholic fatty liver disease, cardiovascular disease and cancer, but all fat is not equal, as storing excess lipid in subcutaneous white adipose tissue (SWAT) is more metabolically favorable than in visceral fat. Here, we uncover a critical role for mTORC2 in setting SWAT lipid handling capacity. We find that subcutaneous white preadipocytes differentiating without the essential mTORC2 subunit Rictor upregulate mature adipocyte markers but develop a striking lipid storage defect resulting in smaller adipocytes, reduced tissue size, lipid re-distribution to visceral and brown fat, and sex-distinct effects on systemic metabolic fitness. Mechanistically, mTORC2 promotes transcriptional upregulation of select lipid metabolism genes controlled by PPARγ and ChREBP, including genes that control lipid uptake, synthesis, and degradation pathways as well as Akt2, which encodes a major mTORC2 substrate and insulin effector. Further exploring this pathway may uncover new strategies to improve insulin sensitivity.

Product Number
Product Description

3-异丁基-1-甲基黄嘌呤, ≥99% (HPLC), powder
游离甘油试剂, used for quantitative enzymatic determination of glycerol
胰岛素 人, recombinant, expressed in yeast (proprietary host)
罗格列酮, ≥98% (HPLC)
油红O, certified by the Biological Stain Commission
丙酮二酸钠 一水合物, ≥98.0% (RT)
Anti-AS160 (Rab-GAP) Antibody, serum, Upstate®
抗SREBP1抗体,克隆2121, clone 2121, Upstate®, from mouse