Merck
  • 主页
  • 查找结果
  • Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitment.

Leptin reduces in vitro cementoblast mineralization and survival as well as induces PGE2 release by ERK1/2 commitment.

Clinical oral investigations (2020-08-21)
G Ruiz-Heiland, J W Yong, J von Bremen, S Ruf
摘要

Juvenile obesity is a complex clinical condition that is present more and more frequently in the daily orthodontic practice. Over-weighted patients have an impaired bone metabolism, due in part to their increased levels of circulating adipokines. Particularly, leptin has been reported to play a key role in bone physiology. Leptin is ubiquitously present in the body, including blood, saliva, and crevicular fluid. If, and to what extent, it could influence the reaction of cementoblasts during orthodontic-induced forces is yet unknown. OCCM-30 cementoblasts were cultivated under compressive forces using different concentrations of leptin. The expression of ObR, Runx-2, Osteocalcin, Rank-L, Sost, Caspase 3, 8, and 9 were analyzed by RT-PCR. Western blots were employed for protein analysis. The ERK1/2 antagonist FR180204 (Calbiochem) was used and cPLA2 activation, PGE2, and cytochrome C release were further evaluated. In vitro, when compressive forces are applied, leptin promotes ERK1/2 phosphorylation, as well as upregulates PGE2 and caspase 3 and caspase 9 on OCCM cells. Blockade of ERK1/2 impairs leptin-induced PGE2 secretion and reduced caspase 3 and caspase 9 expression. Leptin influences the physiological effect of compressive forces on cementoblasts, exerting in vitro a pro-inflammatory and pro-apoptotic effect. Our findings indicate that leptin exacerbates the physiological effect of compressive forces on cementoblasts promoting the release of PGE2 and increases the rate of cell apoptosis, and thus, increased levels of leptin may influence the inflammatory response during orthodontically induced tooth movement.

材料
货号
品牌
产品描述

Sigma-Aldrich
丽春红S染液(零售包装) 溶液, BioReagent, suitable for electrophoresis, 0.1 % (w/v) in 5% acetic acid
Sigma-Aldrich
茜素红 S, certified by the Biological Stain Commission
Sigma-Aldrich
用于ELISA的碱性磷酸酶黄色(pNPP)液体底物系统, ready to use solution
Sigma-Aldrich
4-硝基苯酚 溶液, 10 mM
Sigma-Aldrich
β-Glycerophosphate, Disodium Salt, Pentahydrate - CAS 13408-09-8 - Calbiochem, A phosphate group donor in matrix mineralization studies that acts as a protein phosphatase inhibitor.