Lysis and protoplast formation of group B streptococci by mutanolysin.

Infection and immunity (1980-06-01)
G B Calandra, R M Cole
摘要

Group B streptococci, refractory to previously tested muralysins under physiological conditions, were successfully converted to protoplasts by use of a recently describede N-acetyl muramidase, mutanolysin, derived from a streptomycete. Purified enzyme was effective, but crude preparations, although degrading cell walls, simultaneously produced peculiar effects of cytoplasmic coagulation, retention of cell shape, loss of some intracellular enzymes, and a rise in optical density. Addition of purified mutanolysin to the array of muralysins (group C streptococcal phage-associated lysin, lysozyme), previously successful in preparing protoplasts of different streptococci, now makes possible enzymatic preparation of protoplasts of streptococci of groups A, B, C. D. G, and H.

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Sigma-Aldrich
变溶菌素 来源于球孢链霉菌 ATCC 21553, lyophilized powder, ≥4000 units/mg protein (biuret), Chromatographically purified
Sigma-Aldrich
变溶菌素 来源于球孢链霉菌 ATCC 21553, free of DNA contaminants, suitable for Microbiome research, lyophilized powder, ≥4000 units/mg protein (biuret)